Cytotoxicity of eight cigarette smoke condensates in three test systems: Comparisons between assays and condensates

Regulatory Toxicology and Pharmacology

Volumn 58, Issue 3, 2010, Pages 428-436

  Richter, Patricia A ^a   Li, Albert P ^b   Polzin, Gregory ^c   Roy, Shambhu K ^d  

^a NATIONAL CENTER FOR CHRONIC DISEASE PREVENTION AND HEALTH PROMOTION   (United States)

^b SITEK Research Laboratories   (United States)

^c NATIONAL CENTER FOR ENVIRONMENTAL HEALTH   (United States)

^d Department of Genetic and Molecular Toxicology   (United States)

Author keywords

Cell growth; Cigarette smoke condensate; Cytotoxicity; Human lung cells

Indexed keywords

CIGARETTE SMOKE CONDENSATE; NICOTINE; TAR; TOBACCO TAR;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL DEATH; CELL GROWTH; CELL STRAIN; CELL STRAIN TK 6; CELL TYPE; CELL VIABILITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOTOXICITY; FEMALE; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MEDIAN EFFECTIVE CONCENTRATION; MICROVASCULAR ENDOTHELIAL CELL; MOUSE; MULTIPLE CELL TYPE COCULTURE SYSTEM; MULTIPLE CYTOTOXICITY ENDPOINT ASSAY; NEUTRAL DYE ASSAY; NONHUMAN; PRIORITY JOURNAL; RESPIRATORY EPITHELIUM; TOXICITY TESTING; ANIMAL; APOPTOSIS; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CHO CELL; COCULTURE; COMPARATIVE STUDY; CRICETULUS; DOSE RESPONSE; DRUG EFFECT; ENDOTHELIUM CELL; EPITHELIUM CELL; HAMSTER; LUNG; MUTAGEN TESTING; NECROSIS; SMOKE; SMOKING; TOBACCO;

NICOTIANA TABACUM;

ANIMALS; APOPTOSIS; CELL DEATH; CELL LINE; CELL PROLIFERATION; CELL SURVIVAL; CHO CELLS; COCULTURE TECHNIQUES; CRICETINAE; CRICETULUS; DOSE-RESPONSE RELATIONSHIP, DRUG; ENDOTHELIAL CELLS; EPITHELIAL CELLS; HUMANS; LUNG; MUTAGENICITY TESTS; NECROSIS; NICOTINE; SMOKE; SMOKING; TARS; TOBACCO;

EID: 79953287659     PISSN: 02732300     EISSN: 10960295     Source Type: Journal    
DOI: 10.1016/j.yrtph.2010.08.009     Document Type: Article

References (46)

1. Andreoli C., Gigante D., Nunziata A. A review of in vitro methods to assess the biological activity of tobacco smoke with the aim of reducing the toxicity of smoke. Toxicol. In Vitro 2003, 17(5-6):587-594.
2. Aoshiba K., Tamaoki J., Nagai A. Acute cigarette smoke exposure induces apoptosis of alveolar macrophages. Am. J. Physiol. Lung Cell Mol. Physiol. 2001, 281(6):L1392-L1401.
3. Battista, S.P., 1976. Cilia toxic components of cigarette smoke. In: Wynder, E.L., Hoffmann, D., Gori, G.B., (Eds.), Smoking and Health 1. Modifying the Risk to the Smoker, Proceedings of the 3rd World Conference on Smoking and Health. New York, June 2-5, 1975; US Department of Health, Education, and Welfare, Public Health Service, National Institutes of Health, National Cancer Institute, Washington, pp. 517-34 (DHEW Publication No. (NIH) 76-1221).
4. Bombick D.W., Ayres P.H., Doolittle D.J. Cytotoxicity assessment of whole smoke and vapor phase of mainstream and sidestream cigarette smoke from three Kentucky reference cigarettes. Toxicol. Methods 1997, 7(3):177-190.
5. Bombick D.W., Bombick B.R., Ayres P.H., Putnam K., Avalos J., Borgerding M.F., Doolittle D.J. Evaluation of the genotoxic and cytotoxic potential of mainstream whole smoke and smoke condensate from a cigarette containing a novel carbon filter. Fundam. Appl. Toxicol. 1997, 39(1):11-17.
6. Bombick D.W., Putnam K., Doolittle D.J. Comparative cytotoxicity studies of smoke condensates from different types of cigarettes and tobaccos. Toxicol. In Vitro 1998, 12(3):241-249.
7. Borenfreund E., Puerner J.A. Toxicity determined in vitro by morphological alterations and neutral red absorption. Toxicol. Lett. 1985, 24(2-3):119-124.
8. Chen J., Higby R., Tian D., Tan D., Johnson M.D., Xiao Y., Kellar K.J., Feng S., Shields P.G. Toxicological analysis of low-nicotine and nicotine-free cigarettes. Toxicology 2008, 249(2-3):194-203.
9. Counts M.E., Morton M.J., Laffoon S.W., Cox R.H., Lipowicz P.J. Smoke composition and predicting relationships for international commercial cigarettes smoked with three machine-smoking conditions. Regul. Toxicol. Pharmacol. 2005, 41(3):185-227.
10. Curvall M., Enzell C.R., Jansson T., Pettersson B., Thelestam M. Evaluation of the biological activity of cigarette-smoke condensate fractions using six in vitro short-term tests. J. Toxicol. Environ. Health 1984, 14(2-3):163-180.
11. Curvall M., Jansson T., Pettersson B., Hedin A., Enzell C.R. In vitro studies of biological effects of cigarette smoke condensate. I: genotoxic and cytotoxic effects of neutral, semivolatile constituents. Mutat. Res. 1985, 157(2-3):169-180.
12. DeMarini D.M., Gudi R., Szkudlinska A., Rao M., Recio L., Kehl M., Kirby P.E., Polzin G., Richter P.A. Genotoxicity of 10 cigarette smoke condensates in four test systems: comparisons between assays and condensates. Mutat. Res. 2008, 650(1):15-29.
13. Elmore S. Invited review: apoptosis: a review of programmed cell death. Toxicol. Pathol. 2007, 35(4):495-516.
14. Fleiss J.L. Stratification to control for prognostic variables. The Design and Analysis of Clinical Experiments 1986, 149-165. Wiley, New York. J.L. Fleiss (Ed.).
15. Hoffmann, D., Hoffmann, I., 1996. The changing cigarette: chemical studies and bioassays. In: Smoking and Tobacco Control Monograph, No. 13. National Cancer Institute, pp. 159-192 (Chapter 5).
16. Hoffmann, D., Djordjevic, M.V., Brunnemann, K.D., 1996. Changes in cigarette design and composition over time and how they influence the yields of smoke constituents. In: Smoking and Tobacco Control Monograph, No. 7. National Cancer Institute, pp. 15-37 (Chapter 3).
17. Hoshino Y., Mio T., Nagai S., Miki H., Ito L., Izumi T. Cytotoxic effects of cigarette smoke extract on an alveolar type II cell-derived cell line. Am. J. Physiol. Lung Cell Mol. Physiol. 2001, 281(2):L509-L516.
18. Jianlin L., Guohai C., Guojun Z., Jian J., Hangfang H., Juanjuan X., Shu X., Zhijian C., Wei J., Yezhen L., Xiaoxue L., Jiliang H. Assessing cytogenotoxicity of cigarette smoke condensates using three in vitro assays. Mutat. Res. 2009, 677(1-2):21-26.
19. Kanduc D., Mittelman A., Serpico R., Sinigaglia E., Sinha A.A., Natale C., Santacroce R., Di Corcia M.G., Lucchese A., Dini L., Pani P., Santacroce S., Simone S., Bucci R., Farber R.E. Cell death: apoptosis versus necrosis (review). Int. J. Oncol. 2002, 21(1):165-170.
20. Laugesen M., Fowles J. Marlboro ultrasmooth: a potentially reduced exposure product?. Tob. Control 2006, 15(6):430-435.
21. Li A.P. In vitro evaluation of human xenobiotic toxicity: scientific concepts and the novel integrated discrete multiple cell co-culture (IdMOC) technology. ALTEX 2008, 25(1):43-49.
22. Li A.P., Bode C., Sakai Y. A novel in vitro system, the integrated discrete multiple organ cell culture (IdMOC) system, for the evaluation of human drug toxicity: comparative cytotoxicity of tamoxifen towards normal human cells from five major organs and MCF-7 adenocarcinoma breast cancer cells. Chem. Biol. Interact. 2004, 150(1):129-136.
23. Li L., Hamilton R.F., Taylor D.E., Holian A. Acrolein-induced cell death in human alveolar macrophages. Toxicol. Appl. Pharmacol. 1997, 145(2):331-339.
24. Lubet R.A., Kouri R.E., Curren R.A., Putman D.L., Schechtman L.M. Induction of mutagenesis and transformation in BALB/c-3T3 clone A31-1 cells by diverse chemical carcinogens. Environ. Mol. Mutagen. 1990, 16(1):13-20.
25. Luppi F., Aarbious J., van Wetering S., Rahman I., de Boer W.I., Rabe K.R., Hiemst P.S. Effects of cigarette smoke condensate on proliferation and wound closure of bronchial epithelial cells in vitro: role of glutathione. Respir. Res. 2005, 6:140.
26. Maertens R.M., White P.A., Rickert W., Levasseur G., Douglas G.R., Bellier P.V., McNamee J.P., Thuppal V., Walker M., Desjardins S. The genotoxicity of mainstream and sidestream marijuana and tobacco smoke condensates. Chem. Res. Toxicol. 2009, 22(8):1406-1414.
27. Matsukura N., Willey J., Miyashita M., Taffe B., Hoffmann D., Waldren C., Puck T.T., Harris C.C. Detection of direct mutagenicity of cigarette smoke condensate in mammalian cells. Carcinogenesis 1991, 12(4):685-689.
28. Misonou Y., Asahi M., Yokoe S., Miyoshi E., Taniguchi N. Acrolein produces nitric oxide through the elevation of intracellular calcium levels to induce apoptosis in human umbilical vein endothelial cells: implications for smoke angiopathy. Nitric Oxide 2006, 14(2):180-187.
29. National Toxicology Program, 2003. Test Method Protocol for BALB/c 3T3 Neutral Red Uptake Cytotoxicity the National Toxicology Program (NTP) Interagency Center for the Evaluation of Alternative Toxicological Methods (NICEATM). November 4, 2003.
30. Polzin G.M., Zhan L., Hearn B.A., Tavakoli A.D., Vaughan C., Ding Y.S., Ashley D.L., Watson C.H. Effect of charcoal-containing cigarette filters on gas phase volatile organic compounds in mainstream smoke. Tob. Control 2008, 17(Suppl. 1):i10-i16.
31. Pouli A.E., Hatzinikolaou D.G., Piperi C., Stavridou A., Psallidopoulos M.C., Stavrides J.C. The cytotoxic effect of volatile organic compounds of the gas phase of cigarette smoke on lung epithelial cells. Free Radic. Biol. Med. 2003, 34(3):345-355.
32. Putnam K.P., Bombick D.W., Doolittle D.J. Evaluation of eight in vitro assays for assessing the cytotoxicity of cigarette smoke condensate. Toxicol. In vitro 2002, 16(5):599-607.
33. Ramage L., Jones A.C., Whelan C.J. Induction of apoptosis with tobacco smoke and related products in A549 lung epithelial cells in vitro. J. Inflamm. (London, UK) 2006, 3:3.
34. Rickert W.S., Trivedi A.H., Momin R.A., Wright W.G., Lauterbach J.H. Effect of smoking conditions and methods of collection on the mutagenicity and cytotoxicity of cigarette mainstream smoke. Toxicol. Sci. 2007, 96(2):285-293.
35. Rickert W.S., Wright W.G., Trivedi A.H., Momin R.A., Lauterbach J.H. A comparative study of the mutagenicity of various types of tobacco products. Regul. Toxicol. Pharmacol. 2007, 48(3):320-330.
36. Roemer E., Ottmueller T.H., Zenzen V., Wittke S., Radtke F., Blanco L., Carchman R.A. Cytotoxicity, mutagenicity, and tumorigenicity of mainstream smoke from three reference cigarettes machine-smoked to the same yields of total particulate matter per cigarette. Food Chem. Toxicol. 2009, 47(8):1810-1818.
37. Schueller H.M., Al-Wadei H.A., Majidi M. Gamma-aminobutyric acid, a potential tumor suppressor for small airway-derived lung adenocarcinoma. Carcinogenesis 2008, 29(10):1979-1985.
38. Sekhon H.S., Wright J.L., Churg A. Cigarette smoke causes rapid cell proliferation in small airways and associated pulmonary arteries. Am. J. Physiol. 1994, 267(5 Pt. 1):L557-L563.
39. Smith C.J., Perfetti T.A., King J.A. Perspectives on pulmonary inflammation and lung cancer risk in cigarette smokers. Inhal. Toxicol. 2006, 18(9):667-677.
40. Teepe A.G., Beck D.J., Li A.P. Comparison of rat liver parenchymal and nonparenchymal cells in the activation of promutagens. Environ. Mol. Mutagen. 1992, 20(2):134-139.
41. Tewes F.J., Meisgen T.J., Veltel D.J., Roemer E., Patskan G. Toxicological evaluation of an electrically heated cigarette. Part 3: genotoxicity and cytotoxicity of mainstream smoke. J. Appl. Toxicol. 2003, 23:341-348.
42. Thayer P.S., Kensler C.J. Cigarette smoke: charcoal filters reduce components that inhibit growth of cultured human cells. Science 1964, 146(3644):642-644.
43. van der Vaart H., Postma D.S., Timens W., Ten Hacken N.H.T. Acute effects of cigarette smoke on inflammation and oxidative stress: a review. Thorax 2004, 59(8):713-721.
44. Warner K.E., Slade J. Low tar, high toll. Am. J. Public Health 1992, 82(1):17-18.
45. Weiss W., Weiss W.A. Effect of tobacco smoke solutions on paramecium. Arch. Environ. Health 1964, 9:500-504.
46. Wooten J.B., Chouchane S., McGrath T.E. Tobacco smoke constituents affecting oxidative stress. Cigarette Smoke and Oxidative Stress 2006, 6-46. Springer, New York. B. Halliwell, H. Poulsen (Eds.).