Drop-out phagemid vector for switching from phage displayed affinity reagents to expression formats

Analytical Biochemistry

Volumn 412, Issue 2, 2011, Pages 210-216

  Pershad, Kritika ^a   Sullivan, Mark A ^b   Kay, Brian K ^a  

^a UNIVERSITY OF ILLINOIS AT CHICAGO   (United States)

^b UNIVERSITY OF ROCHESTER MEDICAL CENTER   (United States)

Author keywords

Affinity capture; Alkaline phosphatase fusions; Phagemid vector; Religation; Restriction enzymes

Indexed keywords

ALKALINITY; AMINO ACIDS; DIAGNOSIS; DROPS; GENES; PHOSPHATASES; REAGENTS; VECTORS;

AFFINITY REAGENTS; AFFINITY-CAPTURE; ALKALINE PHOSPHATASE; BIOCHEMICAL CHARACTERIZATION; EXPRESSION VECTORS; RELIGATION; RESTRICTION DIGESTION; RESTRICTION ENZYMES;

PHAGE DISPLAY;

ALKALINE PHOSPHATASE; BACTERIAL VECTOR; LACTOFERRIN; POLYHISTIDINE TAG; RECOVERIN; RESTRICTION ENDONUCLEASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CARCINOGENICITY; GENE SEQUENCE; GENETIC CODE; HUMAN; NONHUMAN; PHAGEMID; PRIORITY JOURNAL; PROTEIN BINDING; ZEBRA FISH;

DANIO RERIO;

EID: 79953219400     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.02.006     Document Type: Article

References (37)

1. M.D. Sheets, P. Amersdorfer, R. Finnern, P. Sargent, E. Lindquist, R. Schier, G. Hemingsen, C. Wong, J.C. Gerhart, and J.D. Marks Efficient construction of a large nonimmune phage antibody library: the production of high-affinity human single-chain antibodies to protein antigens Proc. Natl. Acad. Sci. USA 95 1998 6157 6162 (Pubitemid 28248981)
2. J.M. Bliss, M.A. Sullivan, J. Malone, and C.G. Haidaris Differentiation of Candida albicans and Candida dubliniensis by using recombinant human antibody single-chain variable fragments specific for hyphae J. Clin. Microbiol. 41 2003 1152 1160 (Pubitemid 36307122)
3. C. Rothe, S. Urlinger, C. Lohning, J. Prassler, Y. Stark, U. Jager, B. Hubner, M. Bardroff, I. Pradel, M. Boss, R. Bittlingmaier, T. Bataa, C. Frisch, B. Brocks, A. Honegger, and M. Urban The human combinatorial antibody library HuCAL GOLD combines diversification of all six CDRs according to the natural immune system with a novel display method for efficient selection of high-affinity antibodies J. Mol. Biol. 376 2008 1182 1200
4. A. Koide, C.W. Bailey, X. Huang, and S. Koide The fibronectin type III domain as a scaffold for novel binding proteins J. Mol. Biol. 284 1998 1141 1151 (Pubitemid 28558562)
5. M.D. Scholle, J.W. Kehoe, and B.K. Kay Efficient construction of a large collection of phage-displayed combinatorial peptide libraries Comb. Chem. High Throughput Screen. 8 2005 545 551 (Pubitemid 41242959)
6. A. Ernst, S.L. Sazinsky, S. Hui, B. Currell, M. Dharsee, S. Seshagiri, G.D. Bader, and S.S. Sidhu Rapid evolution of functional complexity in a domain family Sci. Signal. 2 2009 ra50
7. T. Huber, D. Steiner, D. Rothlisberger, and A. Pluckthun In vitro selection and characterization of DARPins and Fab fragments for the co-crystallization of membrane proteins: the Na(+)-citrate symporter CitS as an example J. Struct. Biol. 159 2007 206 221 (Pubitemid 47095399)
8. S.E. Hufton, N. van Neer, T. van den Beuken, J. Desmet, E. Sablon, and H.R. Hoogenboom Development and application of cytotoxic T lymphocyte-associated antigen 4 as a protein scaffold for the generation of novel binding ligands FEBS Lett. 475 2000 225 231 (Pubitemid 30408458)
9. F.Y. Nilsson, and V. Tolmachev Affibody molecules: new protein domains for molecular imaging and targeted tumor therapy Curr. Opin. Drug Discov. Dev. 10 2007 167 175 (Pubitemid 46438646)
10. A. Skerra Alternative binding proteins: anticalins - harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities FEBS J. 275 2008 2677 2683 (Pubitemid 351678679)
11. G.P. Smith, and V.A. Petrenko Phage display Chem. Rev. 97 1997 391 410 (Pubitemid 127659250)
12. J. Andris-Widhopf, C. Rader, P. Steinberger, R. Fuller, and C.F. Barbas 3rd Methods for the generation of chicken monoclonal antibody fragments by phage display J. Immunol. Methods 242 2000 159 181
13. H.Y. Yang, K.J. Kang, J.E. Chung, and H. Shim Construction of a large synthetic human scFv library with six diversified CDRs and high functional diversity Mol. Cells 27 2009 225 235
14. T.J. Vaughan, A.J. Williams, K. Pritchard, J.K. Osbourn, A.R. Pope, J.C. Earnshaw, J. McCafferty, R.A. Hodits, J. Wilton, and K.S. Johnson Human antibodies with sub-nanomolar affinities isolated from a large non-immunized phage display library Nat. Biotechnol. 14 1996 309 314
15. B.K. Kay, J. Kasanov, and M. Yamabhai Screening phage-displayed combinatorial peptide libraries Methods 24 2001 240 246 (Pubitemid 32846430)
16. L. Turunen, K. Takkinen, H. Soderlund, and T. Pulli Automated panning and screening procedure on microplates for antibody generation from phage display libraries J. Biomol. Screen. 14 2009 282 293
17. D.J. Schofield, A.R. Pope, V. Clementel, J. Buckell, S. Chapple, K.F. Clarke, J.S. Conquer, A.M. Crofts, S.R. Crowther, M.R. Dyson, G. Flack, G.J. Griffin, Y. Hooks, W.J. Howat, A. Kolb-Kokocinski, S. Kunze, C.D. Martin, G.L. Maslen, J.N. Mitchell, M. O'Sullivan, R.L. Perera, W. Roake, S.P. Shadbolt, K.J. Vincent, A. Warford, W.E. Wilson, J. Xie, J.L. Young, and J. McCafferty Application of phage display to high throughput antibody generation and characterization Genome Biol. 8 2007 R254
18. K. Pershad, J.D. Pavlovic, S. Graslund, P. Nilsson, K. Colwill, A. Karatt-Vellatt, D.J. Schofield, M.R. Dyson, T. Pawson, B.K. Kay, and J. McCafferty Generating a panel of highly specific antibodies to 20 human SH2 domains by phage display Protein Eng. Des. Sel. 23 2010 279 288
19. W. Wels, I.M. Harwerth, M. Zwickl, N. Hardman, B. Groner, and N.E. Hynes Construction, bacterial expression and characterization of a bifunctional single-chain antibody-phosphatase fusion protein targeted to the human erbB-2 receptor Biotechnology (NY) 10 1992 1128 1132
20. C.D. Martin, G. Rojas, J.N. Mitchell, K.J. Vincent, J. Wu, J. McCafferty, and D.J. Schofield A simple vector system to improve performance and utilisation of recombinant antibodies BMC Biotechnol. 6 2006 46
21. B.H. Muller, D. Chevrier, J.C. Boulain, and J.L. Guesdon Recombinant single-chain Fv antibody fragment-alkaline phosphatase conjugate for one-step immunodetection in molecular hybridization J. Immunol. Methods 227 1999 177 185 (Pubitemid 29408715)
22. R.M. Wright, D. Dudas, B. Gavin, D. Dottavio, J.M. Hexham, and P. Lake A high-capacity alkaline phosphatase reporter system for the rapid analysis of specificity and relative affinity of peptides from phage-display libraries J. Immunol. Methods 253 2001 223 232 (Pubitemid 32522529)
23. A. Gonzalez-Techera, M. Umpierrez-Failache, S. Cardozo, G. Obal, O. Pritsch, J.A. Last, S.J. Gee, B.D. Hammock, and G. Gonzalez-Sapienza High-throughput method for ranking the affinity of peptide ligands selected from phage display libraries Bioconjug. Chem. 19 2008 993 1000 (Pubitemid 351748306)
24. Z. Han, E. Karatan, M.D. Scholle, J. McCafferty, and B.K. Kay Accelerated screening of phage-display output with alkaline phosphatase fusions Comb. Chem. High Throughput Screen. 7 2004 55 62 (Pubitemid 38332575)
25. C.G. Haidaris, J. Malone, L.A. Sherrill, J.M. Bliss, A.A. Gaspari, R.A. Insel, and M.A. Sullivan Recombinant human antibody single chain variable fragments reactive with Candida albicans surface antigens J. Immunol. Methods 257 2001 185 202
26. S.S. Sidhu, H.B. Lowman, B.C. Cunningham, and J.A. Wells Phage display for selection of novel binding peptides Methods Enzymol. 328 2000 333 363
27. Z.Z. Shi, J.W. Zhang, and S. Zheng What we know about ST13, a co-factor of heat shock protein, or a tumor suppressor? J. Zhejiang Univ. Sci. B 8 2007 170 176
28. A.V. Bazhin, D. Schadendorf, P.P. Philippov, and S.B. Eichmuller Recoverin as a cancer-retina antigen Cancer Immunol. Immunother. 56 2007 110 116 (Pubitemid 44812453)
29. E. Karatan, M. Merguerian, Z. Han, M.D. Scholle, S. Koide, and B.K. Kay Molecular recognition properties of FN3 monobodies that bind the Src SH3 domain Chem. Biol. 11 2004 835 844 (Pubitemid 38836899)
30. A. Koide, R.N. Gilbreth, K. Esaki, V. Tereshko, and S. Koide High-affinity single-domain binding proteins with a binary-code interface Proc. Natl. Acad. Sci. USA 104 2007 6632 6637 (Pubitemid 47175552)
31. M.D. Scholle, F.R. Collart, and B.K. Kay In vivo biotinylated proteins as targets for phage-display selection experiments Protein Expr. Purif. 37 2004 243 252 (Pubitemid 39027267)
32. Y. Kwon, Z. Han, E. Karatan, M. Mrksich, and B.K. Kay Antibody arrays prepared by cutinase-mediated immobilization on self-assembled monolayers Anal. Chem. 76 2004 5713 5720 (Pubitemid 39313318)
33. M. Ohshima, K. Inoue, H. Hayashi, D. Tsuji, M. Mizugaki, and K. Itoh Generation of AcGFP fusion with single-chain Fv selected from a phage display library constructed from mice hyperimmunized against 5-methyl 2′-deoxycytidine Protein Eng. Des. Sel. 23 2010 881 888
34. G.V. Los, L.P. Encell, M.G. McDougall, D.D. Hartzell, N. Karassina, C. Zimprich, M.G. Wood, R. Learish, R.F. Ohana, M. Urh, D. Simpson, J. Mendez, K. Zimmerman, P. Otto, G. Vidugiris, J. Zhu, A. Darzins, D.H. Klaubert, R.F. Bulleit, and K.V. Wood HaloTag: a novel protein labeling technology for cell imaging and protein analysis ACS Chem. Biol. 3 2008 373 382
35. H.R. Hoogenboom, A.D. Griffiths, K.S. Johnson, D.J. Chiswell, P. Hudson, and G. Winter Multi-subunit proteins on the surface of filamentous phage: methodologies for displaying antibody (Fab) heavy and light chains Nucleic Acids Res. 19 1991 4133 4137 (Pubitemid 21912784)
36. Q. Zhao, Y.W. Chan, S.S. Lee, and W.T. Cheung One-step expression and purification of single-chain variable antibody fragment using an improved hexahistidine tag phagemid vector Protein Expr. Purif. 68 2009 190 195
37. S. Hamilton, J. Odili, M.D. Pacifico, G.D. Wilson, and J.M. Kupsch Effect of imidazole on the solubility of a his-tagged antibody fragment Hybrid. Hybridomics 22 2003 347 355 (Pubitemid 38037445)