One-step expression and purification of single-chain variable antibody fragment using an improved hexahistidine tag phagemid vector

Protein Expression and Purification

Volumn 68, Issue 2, 2009, Pages 190-195

  Zhao, Qi ^a   Chan, Yin Wah ^a   Lee, Susanna Sau Tuen ^a   Cheung, Wing Tai ^a  

^a CHINESE UNIVERSITY OF HONG KONG   (Hong Kong)

Author keywords

Antibody; Hexahistidine tag; Immobilized metal affinity chromatography; pCANTAB His; Phage displayed antibody library; Phagemid vector; scFv

Indexed keywords

HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; OLIGOPEPTIDE; PEPTIDE LIBRARY; SINGLE CHAIN ANTIBODIES; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

AFFINITY CHROMATOGRAPHY; ARTICLE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE;

BASE SEQUENCE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; FLOW CYTOMETRY; GENETIC VECTORS; HISTIDINE; HUMANS; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PEPTIDE LIBRARY; RECOMBINANT FUSION PROTEINS; SINGLE-CHAIN ANTIBODIES;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 70349445271     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2009.08.004     Document Type: Article

References (28)

1. Mutuberria R., Satijn S., Huijbers A., Van Der Linden E., Lichtenbeld H., Chames P., Arends J.W., and Hoogenboom H.R. Isolation of human antibodies to tumor-associated endothelial cell markers by in vitro human endothelial cell selection with phage display libraries. J. Immunol. Methods 287 (2004) 31-47
2. Felding-Habermann B., Lerner R.A., Lillo A., Zhuang S., Weber M.R., Arrues S., Gao C., Mao S., Saven A., and Janda K.D. Combinatorial antibody libraries from cancer patients yield ligand-mimetic Arg-Gly-Asp-containing immunoglobulins that inhibit breast cancer metastasis. Proc. Natl. Acad. Sci. USA 101 (2004) 17210-17215
3. Roovers R.C., Van der Linden E., de Bruine A.P., Arends J.W., and Hoogenboom H.R. Identification of colon tumour-associated antigens by phage antibody selections on primary colorectal carcinoma. Eur. J. Cancer 37 (2001) 542-549
4. Krag D.N., Shukla G.S., Shen G.P., Pero S., Ashikaga T., Fuller S., Weaver D.L., Burdette-Radoux S., and Thomas C. Selection of tumor-binding ligands in cancer patients with phage display libraries. Cancer Res. 66 (2006) 7724-7733
5. Trepel M., Arap W., and Pasqualini R. In vivo phage display and vascular heterogeneity: implications for targeted medicine. Curr. Opin. Chem. Biol. 6 (2002) 399-404
6. Cheng M., Chan C.W., Cheung R.C., Bikkavilli R.K., Zhao Q., Au S.W., Chan P.K., Lee S.S., Cheng G., Ho W.K., and Cheung W.T. Cross-reactivity of antibody against SARS-coronavirus nucleocapsid protein with IL-11. Biochem. Biophys. Res. Commun. 338 (2005) 1654-1660
7. Clackson T., Hoogenboom H.R., Griffiths A.D., and Winter G. Making antibody fragments using phage display libraries. Nature 352 (1991) 624-628
8. Nishibori N., Horiuchi H., Furusawa S., and Matsuda H. Humanization of chicken monoclonal antibody using phage-display system. Mol. Immunol. 43 (2006) 634-642
9. Kuepper M.B., Huhn M., Spiegel H., Ma J.K., Barth S., Fischer R., and Finnern R. Generation of human antibody fragments against Streptococcus mutans using a phage display chain shuffling approach. BMC Biotechnol. 5 (2005) 4
10. Yoshinaga K., Matsumoto M., Torikai M., Sugyo K., Kuroki S., Nogami K., Matsumoto R., Hashiguchi S., Ito Y., Nakashima T., and Sugimura K. Ig L-chain shuffling for affinity maturation of phage library-derived human anti-human MCP-1 antibody blocking its chemotactic activity. J. Biochem. 143 (2008) 593-601
11. Yang G.H., Yoon S.O., Jang M.H., and Hong H.J. Affinity maturation of an anti-hepatitis B virus PreS1 humanized antibody by phage display. J. Microbiol. 45 (2007) 528-533
12. Hof D., Cheung K., Roossien H.E., Pruijn G.J., and Raats J.M. A novel subtractive antibody phage display method to discover disease markers. Mol. Cell. Proteomics 5 (2006) 245-255
13. Ruan W., Sassoon A., An F., Simko J.P., and Liu B. Identification of clinically significant tumor antigens by selecting phage antibody library on tumor cells in situ using laser capture microdissection. Mol. Cell. Proteomics 5 (2006) 2364-2373
14. Hoogenboom H.R. Selecting and screening recombinant antibody libraries. Nat. Biotechnol. 23 (2005) 1105-1116
15. Chen Y., Diener K., Patel I.R., Kawooya J.K., Martin G.A., Yamdagni P., Zhang X., Sandrasalphaa A., Sahasrabudhe S., and Busch S.J. Phage display of functional human TNF-alpha converting enzyme catalytic domain: a rapid method for the production of stabilized proteolytic proteins for assay development and high-throughput screening. J. Biomol. Screen. 7 (2002) 433-440
16. Yang L., and Nolan J.P. High-throughput screening and characterization of clones selected from phage display libraries. Cytometry A 71 (2007) 625-631
17. Loset G.A., Kristinsson S.G., and Sandlie I. Reliable titration of filamentous bacteriophages independent of pIII fusion moiety and genome size by using trypsin to restore wild-type pIII phenotype. Biotechniques 44 (2008) 551-554
18. Morohashi K., Arai T., Saito S., Watanabe M., Sakaguchi K., and Sugawara F. A high-throughput phage display screening method using a combination of real-time PCR and affinity chromatography. Comb. Chem. High Throughput Screen. 9 (2006) 55-61
19. Johnsson K., and Ge L. Phage display of combinatorial peptide and protein libraries and their applications in biology and chemistry. Curr. Top. Microbiol. Immunol. 243 (1999) 87-105
20. Krebs B., Rauchenberger R., Reiffert S., Rothe C., Tesar M., Thomassen E., Cao M., Dreier T., Fischer D., Höß A., Inge L., Knappik A., Marget M., Pack P., Meng X.-Q., Schier R., Söhlemann P., Winter J., Wölle J., and Kretzschmar T. High-throughput generation and engineering of recombinant human antibodies. J. Immunol. Methods 254 (2001) 67-84
21. Heitner T., Moor A., Garrison J.L., Marks C., Hasan T., and Marks J.D. Selection of cell binding and internalizing epidermal growth factor receptor antibodies from a phage display library. J. Immunol. Methods 248 (2001) 17-30
22. Bradbury A.R., and Marks J.D. Antibodies from phage antibody libraries. J. Immunol. Methods 290 (2004) 29-49
23. Porath J., Carlsson J., Olsson I., and Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature 258 (1975) 598-599
24. Chaga G.S. Twenty-five years of immobilized metal ion affinity chromatography: past, present and future. J. Biochem. Biophys. Methods 49 (2001) 313-334
25. Bolanos-Garcia V.M., and Davies O.R. Structural analysis and classification of native proteins from E. coli commonly co-purified by immobilised metal affinity chromatography. Biochim. Biophys. Acta 1760 (2006) 1304-1313
26. Casey J.L., Keep P.A., Chester K.A., Robson L., Hawkins R.E., and Begent R.H.J. Purification of bacterially expressed single chain Fv antibodies for clinical applications using metal chelate chromatography. J. Immunol. Methods 179 (1995) 105-116
27. Wülfing C., and Plückthun A. Protein folding in the periplasm of Escherichia coli. Mol. Microbiol. 12 (1994) 685-692
28. Kipriyanov S.M. High-level periplasmic expression and purification of scFvs. Methods Mol. Biol. 178 (2002) 333-341