Autophosphorylation of heme-regulated eukaryotic initiation factor 2α kinase and the role of the modification in catalysis

FEBS Journal

Volumn 278, Issue 6, 2011, Pages 918-928

  Igarashi, Jotaro ^a   Sasaki, Takehiko ^a   Kobayashi, Noriko ^b   Yoshioka, Shinji ^b   Matsushita, Miyuki ^b   Shimizu, Toru ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b Bio Medical Center   (Japan)

Author keywords

autophosphorylation; eIF2 kinase; heme; mass spectrometry; mutation

Indexed keywords

ALANINE; HEMOPROTEIN; MUTANT PROTEIN; PHENYLALANINE; PROTEIN KINASE R; SERINE; THREONINE; TYROSINE;

AMINO TERMINAL SEQUENCE; ARTICLE; AUTOPHOSPHORYLATION; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CONTROLLED STUDY; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE INSERTION; GENE MUTATION; GENE OVEREXPRESSION; MASS SPECTROMETRY; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN DOMAIN; PROTEIN FUNCTION; PROTEIN LOCALIZATION; PROTEIN MODIFICATION; PROTEIN PROTEIN INTERACTION; REGULATORY MECHANISM;

AMINO ACID SEQUENCE; ANIMALS; CATALYSIS; EIF-2 KINASE; ESCHERICHIA COLI; HUMANS; MICE; PHOSPHORYLATION; RECOMBINANT PROTEINS; THREONINE; TYROSINE;

ESCHERICHIA COLI; EUKARYOTA;

EID: 79952526237     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2011.08007.x     Document Type: Article

References (24)

1. Dever TE, (2002) Gene-specific regulation by general translation factors. Cell 108, 545-556. (Pubitemid 34260879)
2. Ron D, &, Harding HP, (2007) eIF2α phosphorylation in cellular stress responses and disease. In Translational Control in Biology and Medicine (, Mathews MB, Sonenberg N, &, Hershey JWB, eds), pp. 345-368. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
3. Dever TE, Dar AC, &, Sicheri F, (2007) The eIF2α kinases. In Translational Control in Biology and Medicine (, Mathews MB, Sonenberg N, &, Hershey JWB, eds), pp 319-344. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
4. Chen J-J, &, London IM, (1995) Regulation of protein synthesis by heme-regulated eIF-2α kinase. Trends Biochem Sci 20, 105-108.
5. Chen J-J, (2000) Heme-regulated eIF2α kinase. In Translational Control of Gene Expression (, Sonenberg N, Hershey JWB, &, Mathews MB, eds), pp 529-546. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
6. Chen J-J, (2007) Regulation of protein synthesis by the heme-regulated eIF2α kinase: relevance to anemias. Blood 109, 2693-2699. (Pubitemid 46482060)
7. Berlanga J, Rivero D, Martín R, Herrero S, Moreno S, &, de Haro C, (2010) The role of the mitogen-activated protein kinase Sty1 in regulation of eIF2α kinases in response to environmental stress in Schizosaccharomyces pombe. Eukaryot Cell 9, 194-207.
8. Berlanga JJ, Herrero S, &, de Haro C, (1998) Characterization of the hemin-sensitive eukaryotic initiation factor 2α kinase from mouse nonerythroid cells. J Biol Chem 273, 32340-32346. (Pubitemid 29177182)
9. de Haro C, Méndez R, &, Santoyo J, (1996) The eIF-2α kinases and the control of protein synthesis. FASEB J 10, 1378-1387. (Pubitemid 26347872)
10. Méndez R, Moreno A, &, de Haro C, (1992) Regulation of heme-controlled eukaryotic polypeptide chain initiation factor 2 α-subunit kinase of reticulocyte lysates. J Biol Chem 267, 11500-11507.
11. Igarashi J, Sato A, Kitagawa T, Yoshimura T, Yamauchi S, Sagami I, &, Shimizu T, (2004) Activation of heme-regulated eukaryotic initiation factor 2α kinase by nitric oxide is induced by the formation of a five-coordinate NO-heme complex: optical absorption, electron spin resonance, and resonance raman spectral studies. J Biol Chem 279, 15752-15762. (Pubitemid 38509260)
12. Su Q, Wang S, Gao H, Kazemi S, Harding HP, Ron D, &, Koromilas AE, (2008) Modulation of the eIF2α kinase PERK by tyrosine phosphorylation. J Biol Chem 283, 469-475.
13. Su Q, Wang S, Baltzis D, Qu L-K, Wong AH-T, &, Koromilas AE, (2006) Tyrosine phosphorylation acts as a molecular switch to full-scale activation of the eIF2α RNA-dependent protein kinase. Proc Natl Acad Sci USA 103, 63-68. (Pubitemid 43076105)
14. Rafie-Kolpin M, Han A-P, &, Chen J-J, (2003) Autophosphorylation of threonine 485 in the activation loop is essential for attaining eIF2α kinase activity of HRI. Biochemistry 42, 6536-6544. (Pubitemid 36665829)
15. Bauer BN, Rafie-Kolpin M, Lu L, Han A, &, Chen J-J, (2001) Multiple autophosphorylation is essential for the formation of the active and stable homodimer of heme-regulated eIF2α kinase. Biochemistry 40, 11543-11551. (Pubitemid 32911297)
16. McKenna SA, Lindhout DA, Shimoike T, &, Puglisi JD, (2007) Biophysical and biochemical investigations of dsRNA-activated kinase PKR. Methods Enzymol 430, 373-396.
17. Igarashi J, Murase M, Iizuka A, Pichierri F, Martinkova M, &, Shimizu T, (2008) Elucidation of the heme-binding site of heme-regulated eIF2α kinase (HRI) and the role of the regulatory motif in heme sensing by spectroscopic and catalytic studies of mutant proteins. J Biol Chem 283, 18782-18791.
18. Lu J, O'Hara EB, Trieselmann BA, Romano PR, &, Dever TE, (1999) The interferon-induced double-stranded RNA-activated protein kinase PKR will phosphorylate serine, threonine, or tyrosine at residue 51 in eukaryotic initiation factor 2α. J Biol Chem 274, 32198-32203.
19. Harding HP, Zhang Y, &, Ron D, (1999) Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase. Nature 397, 271-274. (Pubitemid 29051178)
20. Romano PR, Garcia-Barrio MT, Zhang X, Wang Q, Taylor DR, Zhang F, Herring C, Mathews MB, Qin J, &, Hinnebusch AG, (1998) Autophosphorylation in the activation loop is required for full kinase activity in vivo of human and yeast eukaryotic initiation factor 2α kinases PKR and GCN2. Mol Cell Biol 18, 2282-2297. (Pubitemid 28152676)
21. Daub H, Olsen JV, Bairlein M, Gnad F, Oppermann FS, Körner R, Greff Z, Kéri G, Stemmann O, &, Mann M, (2008) Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle. Mol Cell 31, 438-448.
22. Olsen JV, Blagoev B, Gnad F, Macek B, Kumar C, Mortensen P, &, Mann M, (2006) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. Cell 127, 635-648. (Pubitemid 44647421)
23. Méndez R, &, de Haro C, (1994) Casein kinase II is implicated in the regulation of heme-controlled translational inhibitor of reticulocyte lysates. J Biol Chem 269, 6170-6176. (Pubitemid 24243006)
24. Miksanova M, Igarashi J, Minami M, Sagami I, Yamauchi S, Kurokawa H, &, Shimizu T, (2006) Characterization of heme-regulated eIF2α kinase: roles of the N-terminal domain in the oligomeric state, heme binding, catalysis, and inhibition. Biochemistry 45, 9894-9905. (Pubitemid 44257435)