Elucidation of the heme binding site of heme-regulated eukaryotic initiation factor 2α kinase and the role of the regulatory motif in heme sensing by spectroscopic and catalytic studies of mutant proteins

Journal of Biological Chemistry

Volumn 283, Issue 27, 2008, Pages 18782-18791

  Igarashi, Jotaro ^a   Murase, Motohiko ^a   Iizuka, Aya ^a   Pichierri, Fabio ^a   Martinkova, Marketa ^a,b   Shimizu, Toru ^a  

^a TOHOKU UNIVERSITY   (Japan)

^b CHARLES UNIVERSITY   (Czech Republic)

Author keywords

[No Author keywords available]

Indexed keywords

AMINES; AMINO ACIDS; BINDING ENERGY; BINDING SITES; COORDINATION REACTIONS; DISSOCIATION; ENZYME INHIBITION; FLOW INTERACTIONS; IRON COMPOUNDS; LAWS AND LEGISLATION; MAN MACHINE SYSTEMS; ORGANIC ACIDS; PORPHYRINS; PORT TERMINALS; PROTEINS; RATE CONSTANTS;

AUTOPHOSPHORYLATES; AXIAL LIGANDS; CATALYTIC DOMAINS; DISSOCIATION RATE CONSTANTS; EUKARYOTIC INITIATION FACTORS; HEME BINDINGS; HEME IRONS; INHIBITORY EFFECTS; KINASE DOMAINS; MOLECULAR MECHANISMS; MUTANT PROTEINS; NORMAL CONDITIONS; ON SITES; PROTEIN SURFACES; PROTEIN SYNTHESES; PROTOPORPHYRIN; PULLDOWN ASSAYS; REGULATORY MOTIFS; SPECIFIC BINDINGS; SPECTRAL DATUMS; STRUCTURAL CHANGES; TERMINAL DOMAINS; WILD TYPES;

HEMOGLOBIN;

CYSTEINE; HEME; HEMIN; HISTIDINE; INITIATION FACTOR 2ALPHA; LIGAND; MUTANT PROTEIN; PROTOPORPHYRIN; IRON; PROTEIN KINASE R;

ABSORPTION SPECTROSCOPY; AMINO TERMINAL SEQUENCE; ARTICLE; AUTOPHOSPHORYLATION; BINDING AFFINITY; CIRCULAR DICHROISM; COMPLEX FORMATION; DISSOCIATION; ENZYME ACTIVE SITE; ENZYME MECHANISM; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; PROTEIN TERTIARY STRUCTURE; REGULATORY MECHANISM; WILD TYPE; AMINO ACID SUBSTITUTION; ANIMAL; CHEMISTRY; GENETICS; HUMAN; METABOLISM; MISSENSE MUTATION; PHOSPHORYLATION; PROTEIN BINDING; SITE DIRECTED MUTAGENESIS;

EUKARYOTA;

AMINO ACID MOTIFS; AMINO ACID SUBSTITUTION; ANIMALS; CIRCULAR DICHROISM; EIF-2 KINASE; HEME; HUMANS; IRON; MUTAGENESIS, SITE-DIRECTED; MUTATION, MISSENSE; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY;

EID: 49649105752     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M801400200     Document Type: Article

References (31)

1. Wek, R. C., Jiang, H.-Y., and Anthony, T. G. (2006) Biochem. Soc. Trans. 34, 7-11
2. Hinnebusch, A. G. (2005) Nat. Struct. Mol. Biol. 12, 835-838
3. Connor, J. H., and Lyles, D. S. (2005) J. Biol. Chem. 280, 13512-13519
4. Mathews, M. B., Sonenberg, N., and Hershey, J. W. B. (eds) (2007) Translational Control in Biology and Medicine, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
5. Chen, J. J., and London, I. M. (1995) Trends Biochem. Sci. 20, 105-108
6. Chen, J. J. (2000) in Translational Control of Gene Expression (Soneberg, N., Hershey, J. W. B., and Mathews, M. B., eds) pp. 529-546, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
7. Igarashi, J., Sato, A., Kitagawa, T., Yoshimura, T., Yamauchi, S., Sagami, I., and Shimizu, T. (2004) J. Biol. Chem. 279, 15752-15762
8. Miksanova, M., Igarashi, J., Minami, M., Sagami, I., Yamauchi, S., Kurokawa, H., and Shimizu, T. (2006) Biochemistry 45, 9894-9905
9. Han, X. M., Lee, G., Hefner, C., Maher, J. J., and Correia, M. A. (2005) J. Pharmacol. Exp. Ther. 314, 128-138
10. Liao, M., Pabarcus, M. K., Wang, Y., Hefner, C., Maltby, D. A., Medzihradszky, K. F., Salas-Castillo, S. P., Yan, J., Maher, J. J., and Correia, M. A. (2007) J. Pharmacol. Exp. Ther. 323, 979-989
11. Igarashi, J., Kitanishi, K., Martinkova, M., Murase, M. Iizuka, A., and Shimizu, T. (2008) Acta Chim. Slov. 55, 67-74
12. Matinkova, M., Igarashi, J., and Shimizu, T. (2007) FEBS Lett. 581, 4109-4114
13. Inuzuka, T., Yun, B.-G., Ishikawa, H., Takahashi, S., Hori, H., Matts, R. L., Ishimori, K., and Morishima, I. (2004) J. Biol. Chem. 279, 6778-6782
14. Schaftenaarand, G., and Noordik, J. H. (2000) J. Comput. Aided Mol. Des. 14, 123-134
15. Aono, S. (2003) Acc. Chem. Res. 36, 825-831
16. Uchida, T., and Kitagawa, T. (2005) Acc. Chem. Res. 38, 662-670
17. Sasakura, Y., Yoshimura-Suzuki, T., Kurokawa, H., and Shimizu, T. (2006) Acc. Chem. Res. 39, 37-43
18. Zhang, L., and Guarente, L. (1995) EMBO J. 14, 313-320
19. Ishikawa, H., Kato, M., Hori, H., Ishimori, K., Kirisato, T., Tokunaga, F., and Iwai, K. (2005) Mol. Cell 19, 171-181
20. Uma, S., Matts, R. L., Guo, Y., White, S., and Chan, J. J. (2000) Eur. J. Biochem. 267, 498-506
21. Yang, J., Ishimori, K., and O'Brian, M. R. (2005) J. Biol. Chem. 280, 7671-7676
22. Dar, A. C., Dever, T. E., and Sicheri F. (2005) Cell 122, 887-900
23. Rafie-Kolpin, M., Chefalo, P. J., Hussain, Z., Hahn, J., Uma, S., Matts, R. L., and Chen, J. J. (2000) J. Biol. Chem. 275, 5171-5178
24. Zhan, K., Vattem, K. M., Bauer, B. N., Dever, T. E., Chen, J. J., and Wek, R. C. (2002) Mol. Cell. Biol. 22, 7134-7146
25. Ward, J. J., Sodhi, J. S., McGuffin, L. J., Buxton, B. F., and Jones, D. T. (2004) J. Mol. Biol. 337, 635-645
26. Hu, R. G., Wang, H., Xia, Z., and Varshavsky, A. (2008) Proc. Natl. Acad. Sci. U. S. A. 105, 76-81
27. Igarashi, J., Sato, A., Kitagawa, T., Sagami, I., and Shimizu, T. (2003) Biochim. Biophys. Acta 1650, 99-104
28. Koenig, D. F. (1965) Acta Crystallogr. 18, 663-673
29. Hsu, M. C., and Woody, R. W. (1971) J. Am. Chem. Soc. 93, 3515-3525
30. Kiefl, C., Screerama, N., Haddad, R., Sun, L., Jentzen, W., Lu, Y., Qiu, Y., Shelnutt, J. A., and Woody, R. W. (2002) J. Am. Chem. Soc. 124, 3385-3394
31. Hargrove, M. S., Singleton, E. W., Quillin, M. L., Ortiz, L. A., Phillips, Jr., G. N., Olson, J. S., and Mathews, A. J. (1994) J. Biol. Chem. 269, 4207-4214