Allicin from garlic neutralizes the hemolytic activity of intra- and extra-cellular pneumolysin O in vitro

Toxicon

Volumn 57, Issue 4, 2011, Pages 540-545

  Arzanlou, M ^a   Bohlooli, S ^a   Jannati, E ^b   Mirzanejad Asl, H ^a  

^a ARDABIL UNIVERSITY OF MEDICAL SCIENCES   (Iran)

^b ISLAMIC AZAD UNIVERSITY   (Iran)

Author keywords

Allicin; Garlic extract; Hemolytic activity; Pneumolysin O; Streptococcus pneumoniae

Indexed keywords

ALLICIN; GARLIC EXTRACT; PNEUMOLYSIN; PNEUMOLYSIN O; UNCLASSIFIED DRUG; WATER;

ANTIBACTERIAL ACTIVITY; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL CELL; BINDING AFFINITY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DRUG BINDING; HEMOLYSIS; IN VITRO STUDY; MINIMUM INHIBITORY CONCENTRATION; NONHUMAN; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE;

ANTI-INFECTIVE AGENTS; BACTERIAL PROTEINS; DITHIOTHREITOL; DRUG ANTAGONISM; ERYTHROCYTES; GARLIC; HEMOGLOBINS; HEMOLYSIS; HUMANS; MICROBIAL SENSITIVITY TESTS; OXIDATION-REDUCTION; STREPTOCOCCUS PNEUMONIAE; STREPTOLYSINS; SULFINIC ACIDS;

ALLIUM SATIVUM; BACTERIA (MICROORGANISMS); STREPTOCOCCUS PNEUMONIAE;

EID: 79952362439     PISSN: 00410101     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxicon.2010.12.009     Document Type: Article

References (41)

1. Al-Khatib I.M.H., Hanifa Moursi S.A., Mehdi A.W.R., Al-Shabibi M.M. Gas-liquid chromatographic determination of fatty acids and sterols of selected Iraqi foods. J. Food Compos. Anal. 1987, 1:59-64.
2. Alexander J.E., Lock R.A., Peeters C.C., Poolman J.T., Andrew P.W., Mitchell T.J., Hansman D., Paton J.C. Immunization of mice with pneumolysin toxoid confers a significant degree of protection against at least nine serotypes of Streptococcus pneumoniae. Infect. Immun. 1994, 62:5683-5688.
3. Anderson R., Steel H.C., Cockeran R., Smith A.M., von Gottberg A., de Gouveia L., Brink A., Klugman K.P., Mitchell T.J., Feldman C. Clarithromycin alone and in combination with ceftriaxone inhibits the production of pneumolysin by both macrolide-susceptible and macrolide-resistant strains of Streptococcus pneumoniae. J. Antimicrob. Chemother. 2007, 59:224-229.
4. Anderson R., Steel H.C., Cockeran R., von Gottberg A., de Gouveia L., Klugman K.P., Mitchell T.J., Feldman C. Comparison of the effects of macrolides, amoxicillin, ceftriaxone, doxycycline, tobramycin and fluoroquinolones, on the production of pneumolysin by Streptococcus pneumoniae in vitro. J. Antimicrob. Chemother. 2007, 60:1155-1158.
5. Ankri S., Mirelman D. Antimicrobial properties of allicin from garlic. Microbe. Infect. 1999, 1:125-129.
6. Ankri S., Miron T., Rabinkov A., Wilchek M., Mirelman D. Allicin from garlic strongly inhibits cysteine proteinases and cytopathic effects of Entamoeba histolytica. Antimicrob. Agents Chemother. 1997, 41:2286-2288.
7. Arzanlou M., Bohlooli S. Inhibition of streptolysin O by allicin, an active component of garlic. J. Med. Microbiol. 2010, 59:1044-1049.
8. Arzanlou M., Bohlooli S. Introducing of green garlic plant as a new source of allicin. Food Chem. 2010, 6:12-15.
9. Bakri I.M., Douglas C.W. Inhibitory effect of garlic extract on oral bacteria. Arch. Oral Biol. 2005, 50:645-651.
10. Braun J.S., Hoffmann O., Schickhaus M., Freyer D., Dagand E., Bermpohl D., Mitchell T.J., Bechmann I., Weber J.R. Pneumolysin causes neuronal cell death through mitochondrial damage. Infect. Immun. 2007, 75:4245-4254.
11. Briles D.E., Hollingshead S.K., Paton J.C., Ades E.W., Novak L., van Ginkel F.W., Benjamin W.H. Immunizations with pneumococcal surface protein A and pneumolysin are protective against pneumonia in a murine model of pulmonary infection with Streptococcus pneumoniae. J. Infect. Dis. 2003, 188:339-348.
12. Cai Y., Wang R., Pei F., Liang B.B. Antibacterial activity of allicin alone and in combination with beta-lactams against Staphylococcus spp. and Pseudomonas aeruginosa. J. Antibiot. 2007, 60:335-338.
13. CLSI Performance Standards for Antimicrobial Susceptibility Testing. Eighteenth Informational Supplement, M100-S18 2008, Clinical and Laboratory Standards Institute, Wayne, PA.
14. Cockeran R., Anderson R., Feldman C. The role of pneumolysin in the pathogenesis of Streptococcus pneumoniae infection. Curr. Opin. Infect. Dis. 2002, 15:235-239.
15. Cockeran R., Anderson R., Feldman C. Pneumolysin in the immunopathogenesis and treatment of pneumococcal disease. Expert Rev. Anti Infect. Ther. 2003, 1:231-239.
16. Coppi A., Cabinian M., Mirelman D., Sinnis P. Antimalarial activity of allicin, a biologically active compound from garlic cloves. Antimicrob. Agents Chemother. 2006, 50:1731-1737.
17. Cutler R.R., Wilson P. Antibacterial activity of a new, stable, aqueous extract of allicin against methicillin-resistant Staphylococcus aureus. Br. J. Biomed. Sci. 2004, 61:71-74.
18. Cutler R.R., Odent M., Hajj-Ahmad H., Maharjan S., Bennett N.J., Josling P.D., Ball V., Hatton P., Dall'Antonia M. In vitro activity of an aqueous allicin extract and a novel allicin topical gel formulation against Lancefield group B streptococci. J. Antimicrob. Chemother. 2009, 63:151-154.
19. Feldman C., Anderson R. A pivotal role for pneumolysin in the immunopathogenesis, treatment and prevention of pneumococcal disease. S. Afr. Med. J. 2007, 97:1141-1145.
20. Feldman C., Anderson R. New insights into pneumococcal disease. Respirology 2009, 14:167-179.
21. Fukuda Y., Yanagihara K., Higashiyama Y., Miyazaki Y., Hirakata Y., Mukae H., Tomono K., Mizuta Y., Tsukamoto K., Kohno S. Effects of macrolides on pneumolysin of macrolide-resistant Streptococcus pneumoniae. Eur. Respir. J. 2006, 27:1020-1025.
22. Garcia-Suarez Mdel M., Cima-Cabal M.D., Florez N., Garcia P., Cernuda-Cernuda R., Astudillo A., Vazquez F., De los Toyos J.R., Mendez F.J. Protection against pneumococcal pneumonia in mice by monoclonal antibodies to pneumolysin. Infect. Immun. 2004, 72:4534-4540.
23. Garcia-Suarez Mdel M., Florez N., Astudillo A., Vazquez F., Villaverde R., Fabrizio K., Pirofski L.A., Mendez F.J. The role of pneumolysin in mediating lung damage in a lethal pneumococcal pneumonia murine model. Respir. Res. 2007, 8:3.
24. Gilbert R.J., Heenan R.K., Timmins P.A., Gingles N.A., Mitchell T.J., Rowe A.J., Rossjohn J., Parker M.W., Andrew P.W., Byron O. Studies on the structure and mechanism of a bacterial protein toxin by analytical ultracentrifugation and small-angle neutron scattering. J. Mol. Biol. 1999, 293:1145-1160.
25. Gonzalez M.R., Bischofberger M., Pernot L., van der Goot F.G., Freche B. Bacterial pore-forming toxins: the (w)hole story?. Cell Mol. Life Sci. 2008, 65:493-507.
26. Hirst R.A., Gosai B., Rutman A., Guerin C.J., Nicotera P., Andrew P.W., O'Callaghan C. Streptococcus pneumoniae deficient in pneumolysin or autolysin has reduced virulence in meningitis. J. Infect. Dis. 2008, 197:744-751.
27. Lang A., Lahav M., Sakhnini E., Barshack I., Fidder H.H., Avidan B., Bardan E., Hershkoviz R., Bar-Meir S., Chowers Y. Allicin inhibits spontaneous and TNF-alpha induced secretion of proinflammatory cytokines and chemokines from intestinal epithelial cells. Clin. Nutr. 2004, 23:1199-1208.
28. Lanzotti V. The analysis of onion and garlic. J. Chromatogr. A 2006, 1112:3-22.
29. Mitchell T.J. Pneumolysin: structure, function and role in disease. The Comprehensive Sourcebook of Bacterial Protein Toxins 1999, 476-495. Academic Press, London. J.E. Alouf, J.H. Freer (Eds.).
30. Mitchell T.J. Virulence factors and the pathogenesis of disease caused by Streptococcus pneumoniae. Res. Microbiol. 2000, 151:413-419.
31. Mohammed B.J., Mitchell T.J., Andrew P.W., Hirst R.A., O'Callaghan C. The effect of the pneumococcal toxin, pneumolysin on brain ependymal cilia. Microb. Pathog. 1999, 27:303-309.
32. Musher D.M. Infections caused by Streptococcus pneumoniae: clinical spectrum, pathogenesis, immunity, and treatment. Clin. Infect. Dis. 1992, 14:801-807.
33. Nollmann M., Gilbert R., Mitchell T., Sferrazza M., Byron O. The role of cholesterol in the activity of pneumolysin, a bacterial protein toxin. Biophys. J. 2004, 86:3141-3151.
34. Palmer M. The family of thiol-activated, cholesterol-binding cytolysins. Toxicon 2001, 39:1681-1689.
35. Paton J.C., Rowan-Kelly B., Ferrante A. Activation of human complement by the pneumococcal toxin pneumolysin. Infect. Immun. 1984, 43:1085-1087.
36. Rabinkov A., Miron T., Konstantinovski L., Wilchek M., Mirelman D., Weiner L. The mode of action of allicin: trapping of radicals and interaction with thiol containing proteins. Biochim. Biophys. Acta 1998, 1379:233-244.
37. Rubins J.B., Duane P.G., Clawson D., Charboneau D., Young J., Niewoehner D.E. Toxicity of pneumolysin to pulmonary alveolar epithelial cells. Infect. Immun. 1993, 61:1352-1358.
38. Spreer A., Kerstan H., Bottcher T., Gerber J., Siemer A., Zysk G., Mitchell T.J., Eiffert H., Nau R. Reduced release of pneumolysin by Streptococcus pneumoniae in vitro and in vivo after treatment with nonbacteriolytic antibiotics in comparison to ceftriaxone. Antimicrob. Agents Chemother. 2003, 47:2649-2654.
39. Walker J.A., Allen R.L., Falmagne P., Johnson M.K., Boulnois G.J. Molecular cloning, characterization, and complete nucleotide sequence of the gene for pneumolysin, the sulfhydryl-activated toxin of Streptococcus pneumoniae. Infect. Immun. 1987, 55:1184-1189.
40. Wellmer A., Zysk G., Gerber J., Kunst T., Von Mering M., Bunkowski S., Eiffert H., Nau R. Decreased virulence of a pneumolysin-deficient strain of Streptococcus pneumoniae in murine meningitis. Infect. Immun. 2002, 70:6504-6508.
41. Wills E.D. Enzyme inhibition by allicin, the active principle of garlic. Biochem. J. 1956, 63:514-520.