Quantification of mRNA Using Real-Time PCR and Western Blot Analysis of MAPK Events in Chondrocyte/Agarose Constructs

Methods in Molecular Biology

Volumn 695, Issue , 2011, Pages 77-97

  Lee, David A ^a   Brand, June ^b   Salter, Donald ^b   Akanji, Oto Ola ^a   Chowdhury, Tina T ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Agarose; cDNA; Chondrocyte; MAPK; Mechanotransduction; mRNA; RT qPCR; Western blotting

Indexed keywords

COMPLEMENTARY DNA; INDUCIBLE NITRIC OXIDE SYNTHASE; INTERLEUKIN 1BETA; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; RNA DIRECTED DNA POLYMERASE; SEPHAROSE;

ANIMAL; ARTICLE; BIOSYNTHESIS; CARTILAGE CELL; CATTLE; CELL CULTURE; CELL SEPARATION; CHEMISTRY; DRUG EFFECT; ENZYMOLOGY; GENE EXPRESSION REGULATION; GENETICS; METABOLISM; METHODOLOGY; PHOSPHORYLATION; POLYACRYLAMIDE GEL ELECTROPHORESIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TIME; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; CATTLE; CELL SEPARATION; CELLS, CULTURED; CHONDROCYTES; DNA, COMPLEMENTARY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; INTERLEUKIN-1BETA; MITOGEN-ACTIVATED PROTEIN KINASES; NITRIC OXIDE SYNTHASE TYPE II; PHOSPHORYLATION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER; RNA-DIRECTED DNA POLYMERASE; SEPHAROSE; TIME FACTORS;

MLCS; MLOWN;

EID: 79952199423     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-60761-984-0_6     Document Type: Chapter

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