Dynamic compression alters NFκB activation and IκB-α expression in IL-1β-stimulated chondrocyte/agarose constructs

Inflammation Research

Volumn 59, Issue 1, 2010, Pages 41-52

  Akanji, O O ^a   Sakthithasan, P ^a   Salter, D M ^b   Chowdhury, T T ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

Cytokines; Inflammatory mediators; Mechanobiology; NFkB; Osteoarthritis

Indexed keywords

1 PYRROLIDINE DITHIOCARBAMATE; AGAROSE; DITHIOCARBAMIC ACID DERIVATIVE; I KAPPA B ALPHA; IMMUNOGLOBULIN ENHANCER BINDING PROTEIN; INDUCIBLE NITRIC OXIDE SYNTHASE; INTERLEUKIN 1BETA; INTERLEUKIN 4; UNCLASSIFIED DRUG;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CARTILAGE CELL; CELL ISOLATION; COMPRESSION; CONFOCAL MICROSCOPY; CONTROLLED STUDY; GENE EXPRESSION; IMMUNOFLUORESCENCE MICROSCOPY; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; REAL TIME POLYMERASE CHAIN REACTION;

ANIMALS; BIOMECHANICS; CATTLE; CELL NUCLEUS; CELLS, CULTURED; CHONDROCYTES; CYTOPLASM; I-KAPPA B PROTEINS; INTERLEUKIN-1BETA; MODELS, ANIMAL; NF-KAPPA B; SEPHAROSE; SIGNAL TRANSDUCTION; STRESS, MECHANICAL;

EID: 77949266555     PISSN: 10233830     EISSN: 1420908X     Source Type: Journal    
DOI: 10.1007/s00011-009-0068-9     Document Type: Article

References (57)

1. JC Fernandes J Martel-Pelletier JP Pelletier 2002 The role of cytokines in osteoarthritis pathophysiology Biorheology 39 1/2 237 46 1:CAS:528: DC%2BD38XmtFWisL4%3D 12082286
2. F Berenbaum 2004 Signaling transduction: target in osteoarthritis Curr Opin Rheumatol 16 5 616 22 10.1097/01.bor.0000133663.37352.4a 15314504 (Pubitemid 39128334)
3. CJ Malemud 2004 Protein kinases in chondrocyte signalling and osteoarthritis Clin Orthop Relat Res 427 Suppl 145 51 10.1097/01.blo.0000143802. 41885.50 (Pubitemid 39362815)
4. TM Griffin F Guilak 2005 The role of mechanical loading in the onset and progression of osteoarthritis Exerc Sport Sci Rev 33 195 200 10.1097/00003677-200510000-00008 16239837 (Pubitemid 41513204)
5. SJ Millward-Sadler DM Salter 2004 Integrin-dependent signal cascades in chondrocyte mechanotransduction Ann Biomed Eng 32 3 435 46 10.1023/B:ABME. 0000017538.72511.48 1:STN:280:DC%2BD2c7pvFWkug%3D%3D 15095818
6. F Guilak B Fermor FJ Keefe VB Kraus SA Olson DS Pisetsky LA Setton JB Weinberg 2004 The role of biomechanics and inflammation in cartilage repair and injury Clin Orthop Relat Res 423 17 26 10.1097/01.blo.0000131233.83640.91 15232421
7. TL Vincent J Saklatvala 2008 Is the response of cartilage to injury relevant to osteoarthritis? Arthritis Rheum 58 5 1207 10 10.1002/art.23443 1:CAS:528:DC%2BD1cXms1ehurs%3D 18438834 (Pubitemid 351705907)
8. MB Goldring SR Goldring 2007 Osteoarthritis J Cell Physiol 213 3 626 34 10.1002/jcp.21258 1:CAS:528:DC%2BD2sXht12jur7N 17786965 (Pubitemid 350058254)
9. J Saklatvala 2007 Inflammatory signalling in cartilage. MAPK and NFκB pathways in chondrocytes and the use of inhibitors for research into pathogenesis and therapy of OA Curr Drug Targets 8 305 13 10.2174/ 138945007779940115 1:CAS:528:DC%2BD2sXhs1ygsr0%3D 17305508 (Pubitemid 46299678)
10. CJ Malemud 2007 Inhibitors of stress-activated protein/mitogen-activated protein kinase pathways Curr Opin Pharmacol 7 3 339 43 10.1016/j.coph.2006.11. 012 1:CAS:528:DC%2BD2sXlsF2nt7k%3D 17398158 (Pubitemid 46731194)
11. M Kobayashi GR Squires A Mousa M Tanzer DJ Zukor J Antoniou U Feige AR Poole 2005 Role of interleukin-1 and tumor necrosis factor alpha in matrix degradation of human osteoarthritic cartilage Arthritis Rheum 52 1 128 35 10.1002/art.20776 1:CAS:528:DC%2BD2MXht1Kisbk%3D 15641080 (Pubitemid 40129241)
12. PE Auron 1998 The interleukin 1 receptor: ligand interactions and signal transduction Cytokine Growth Factor Rev 9 3/4 221 37 10.1016/S1359-6101(98) 00018-5 1:CAS:528:DyaK1MXmtlKjsA%3D%3D 9918122 (Pubitemid 29019708)
13. R Newton DA Stevens LA Hart M Lindsay IM Adcock PJ Barnes 1997 Superinduction of COX-2 mRNA by cycloheximide and interleukin-1beta involves increased transcription and correlates with increased NF-kappaB and JNK activation FEBS Lett 418 1/2 135 8 10.1016/S0014-5793(97)01362-8 1:CAS:528:DyaK2sXnsVOqtbg%3D 9414112 (Pubitemid 27514432)
14. Z Fan H Yang B Bau S Söder T Aigner 2006 Role of mitogen-activated protein kinases and NFkappaB on IL-1beta-induced effects on collagen type II, MMP-1 and 13 mRNA expression in normal articular human chondrocytes Rheumatol Int 26 10 900 3 10.1007/s00296-006-0114-7 1:CAS:528:DC%2BD28XmtFCgu7s%3D 16468044 (Pubitemid 43962119)
15. A Liacini J Sylvester WQ Li M Zafarullah 2002 Inhibition of IL-1 stimulated MAP kinases, AP-1 and NF-kappa B transcription factors down-regulates MMP gene expression in articular chondrocytes Matrix Biol 21 251 62 10.1016/S0945-053X(02)00007-0 1:CAS:528:DC%2BD38XjsFygsbc%3D 12009331 (Pubitemid 34497016)
16. JA Mengshol MP Vincenti CI Coon A Barchowsky CE Brinckerhoff 2000 IL-1 induction of MMP-13 gene expression in chondrocytes requires p38 c-Jun N-terminal kinase and nuclear factor kappaB: differential regulation of collagenase 1 and collagenase 3 Arthritis Rheum 43 801 11 10.1002/1529- 0131(200004)43:4<801::AID-ANR10>3.0.CO;2-4 1:CAS:528:DC%2BD3cXivFOitr0%3D 10765924 (Pubitemid 30677570)
17. R Tsutsumi H Ito T Hiramitsu K Nishitani M Akiyoshi T Kitaori T Yasuda T Nakamura 2008 Celecoxib inhibits production of MMP and NO via down-regulation of NF-kappaB and JNK in a PGE2 independent manner in human articular chondrocytes Rheumatol Int 28 8 727 36 10.1007/s00296-007-0511-6 1:CAS:528: DC%2BD1cXlsFyjsr0%3D 18080123
18. JA Roman-Blas SA Jimenez 2006 NF-kappaB as a potential therapeutic target in osteoarthritis and rheumatoid arthritis Osteoarthr Cartil 14 9 839 48 10.1016/j.joca.2006.04.008 1:STN:280:DC%2BD28vmvVCnsg%3D%3D 16730463 (Pubitemid 44138675)
19. Aigner T, McKenna L, Zien A, Fan Z, Gebhard PM, Zimmer R. Gene expression profiling of serum- and interleukin-1 beta-stimulated primary human adult articular chondrocytes-a molecular analysis based on chondrocytes isolated from one donor. Cytokine. 2005;31(3):227-40.
20. K Brown S Park T Kanno G Franzoso U Siebenlist 1993 Mutual regulation of the transcriptional activator NF-kappa B and its inhibitor, I kappa B-alpha Proc Natl Acad Sci USA 90 6 2532 6 10.1073/pnas.90.6.2532 1:CAS:528: DyaK3sXktVagtbo%3D 8460169
21. U Zabel T Henkel MS Silva PA Baeuerle 1993 Nuclear uptake control of NF-kappa B by MAD-3, an I kappa B protein present in the nucleus EMBO J 12 1 201 11 1:CAS:528:DyaK3sXht1WmsrY%3D 7679069
22. Bottero V, Imbert V, Frelin C, Formento JL, Peyron JF. Monitoring NF-kappa B transactivation potential via real-time PCR quantification of I kappa B-alpha gene expression. Mol Diagn. 2003;7(3/4):187-94.
23. M Karin A Lin 2002 NF-kappaB at the crossroads of life and death Nat Immunol 3 3 221 7 10.1038/ni0302-221 1:CAS:528:DC%2BD38XitVWlt7g%3D 11875461 (Pubitemid 34213423)
24. MS Rodriguez J Thompson RT Hay C Dargemont 1999 Nuclear retention of IkappaBalpha protects it from signal-induced degradation and inhibits nuclear factor kappaB transcriptional activation J Biol Chem 274 13 9108 15 10.1074/jbc.274.13.9108 1:CAS:528:DyaK1MXitFyru7Y%3D 10085161 (Pubitemid 29164718)
25. S Agarwal J Deschner P Long A Verma C Hofman CH Evans N Piesco 2004 Role of NF-kappaB transcription factors in anti-inflammatory and pro-inflammatory actions of mechanical signals Arthritis Rheum 50 3541 8 10.1002/art.20601 1:CAS:528:DC%2BD2cXhtVOqtrjO 15529376 (Pubitemid 39488679)
26. J Deschner CR Hofman NP Piesco S Agarwal 2003 Signal transduction by mechanical strain in chondrocytes Curr Opin Clin Nutr Metab Care 6 289 93 10.1097/00075197-200305000-00004 1:CAS:528:DC%2BD3sXltVyisrw%3D 12690261 (Pubitemid 40684605)
27. TT Chowdhury DL Bader DA Lee 2006 Anti-inflammatory effects of IL-4 and dynamic compression in IL-1beta stimulated chondrocytes Biochem Biophys Res Commun 339 1 241 7 10.1016/j.bbrc.2005.11.016 1:CAS:528:DC%2BD2MXht1OrsbbE 16297873 (Pubitemid 41697541)
28. H Doi K Nishida M Yorimitsu T Komiyama Y Kadota T Tetsunaga A Yoshida S Kubota M Takigawa T Ozaki 2008 Interleukin-4 downregulates the cyclic tensile stress-induced matrix metalloproteinases-13 and cathepsin B expression by rat normal chondrocytes Acta Med Okayama 62 2 119 26 1:CAS:528:DC%2BD1cXmt1eltr4%3D 18464888
29. S Madhavan M Anghelina B Rath-Deschner E Wypasek A John J Deschner N Piesco S Agarwal 2006 Biomechanical signals exert sustained attenuation of proinflammatory gene induction in articular chondrocytes Osteoarthr Cartil 14 1023 32 10.1016/j.joca.2006.03.016 1:STN:280:DC%2BD28rjvVyiug%3D%3D 16731008 (Pubitemid 44309417)
30. TT Chowdhury S Arghandawi J Brand OO Akanji DL Bader DM Salter DA Lee 2008 Dynamic compression counteracts IL-1beta induced inducible nitric oxide synthase and cyclo-oxygenase-2 expression in chondrocyte/agarose constructs Arthritis Res Ther 10 2 R35 10.1186/ar2389 1:CAS:528:DC%2BD1cXms1aktLY%3D 18348730
31. DA Lee DL Bader 1997 Compressive strains at physiological frequencies influence the metabolism of chondrocytes seeded in agarose J Orthop Res 15 181 8 10.1002/jor.1100150205 9167619 (Pubitemid 27220534)
32. DA Lee MM Knight 2004 Mechanical loading of chondrocytes embedded in 3D constructs: in vitro methods for assessment of morphological and metabolic response to compressive strain Methods Mol Med 100 307 24 1:CAS:528: DC%2BD2cXotFahs7c%3D 15280603
33. TT Chowdhury DM Salter DL Bader DA Lee 2008 Signal transduction pathways involving p38 MAPK, JNK, NFkappaB and AP-1 influences the response of chondrocytes cultured in agarose constructs to IL-1beta and dynamic compression Inflamm Res 57 7 306 13 10.1007/s00011-007-7126-y 1:CAS:528:DC%2BD1cXhtVSrsL%2FN 18545934
34. 2 and antioxidants have opposite effects on activation of NF-kappa B and AP-1 in intact cells: AP-1 as secondary antioxidant-responsive factor EMBO J 12 5 2005 15 1:CAS:528:DyaK3sXks1GitLY%3D 8491191
35. J Marks-Konczalik SC Chu J Moss 1998 Cytokine-mediated transcriptional induction of the human inducible nitric oxide synthase gene requires both AP-1 and NF-κB binding sites J Biol Chem 273 22201 8 10.1074/jbc.273.35.22201 1:CAS:528:DyaK1cXlvVals7k%3D 9712833 (Pubitemid 28399774)
36. TT Chowdhury DL Bader JC Shelton DA Lee 2003 Temporal regulation of chondrocyte metabolism in agarose constructs subjected to dynamic compression Arch Biochem Biophys 417 1 105 11 10.1016/S0003-9861(03)00340-0 1:CAS:528:DC%2BD3sXmt1Wrtbc%3D 12921786 (Pubitemid 36960055)
37. Raveenthiran SP, Chowdhury TT. Dynamic compression inhibits fibronectin fragment induced iNOS and COX-2 expression in chondrocyte/agarose constructs. Biomech Model Mechanobiol. 2008. doi: 10.1007/s10237-008-0134-1
38. MW Pfaffl GW Horgan L Dempfle 2002 Relative expression software tool (REST) for group wise comparison and statistical analysis of relative expression results in real time PCR Nucl Acids Res 30 e3 10.1093/nar/30.9.e36
39. RM Clancy PF Gomez SB Abramson 2004 Nitric oxide sustains nuclear factor kappaB activation in cytokine-stimulated chondrocytes Osteoarthr Cartil 12 7 552 8 10.1016/j.joca.2004.04.003 1:STN:280:DC%2BD2czhvFOqug%3D%3D 15219570 (Pubitemid 38950265)
40. Delhase M, Hayakawa M, Chen Y, Karin M. Positive and negative regulation of IkappaB kinase activity through IKKbeta subunit phosphorylation. Science. 1999;284(5412):309-13
41. ML Schmitz MA dos Santos Silva PA Baeuerle 1995 Transactivation domain 2 (TA2) of p65 NF-kappa B similarity to TA1 and phorbol ester-stimulated activity and phosphorylation in intact cells J Biol Chem 270 26 15576 84 10.1074/jbc.270.26.15576 1:CAS:528:DyaK2MXmslaqurY%3D 7797554
42. F Carlotti R Chapman SK Dower EE Qwarnstrom 1999 Activation of nuclear factor kappaB in single living cells. Dependence of nuclear translocation and anti-apoptotic function on EGFPRELA concentration J Biol Chem 274 53 37941 9 10.1074/jbc.274.53.37941 1:CAS:528:DC%2BD3cXkt1Gmuw%3D%3D 10608861 (Pubitemid 30026862)
43. GJ Ding PA Fischer RC Boltz JA Schmidt JJ Colaianne A Gough RA Rubin DK Miller 1998 Characterization and quantitation of NF-kappaB nuclear translocation induced by interleukin-1 and tumor necrosis factor-alpha: development and use of a high capacity fluorescence cytometric system J Biol Chem 273 44 28897 905 10.1074/jbc.273.44.28897 1:CAS:528:DyaK1cXntlahsrY%3D 9786892 (Pubitemid 28507582)
44. MB Goldring M Otero K Tsuchimochi K Ijiri Y Li 2008 Defining the roles of inflammatory and anabolic cytokines in cartilage metabolism Ann Rheum Dis 67 Suppl 3 iii75 82 10.1136/ard.2008.098764 1:CAS:528:DC%2BD1MXjt1OlsA%3D%3D 19022820
45. PA Scherle MA Pratta WS Feeser EJ Tancula EC Arner 1997 The effects of IL-1 on mitogen-activated protein kinases in rabbit articular chondrocytes Biochem Biophys Res Commun 230 573 7 10.1006/bbrc.1996.5985 1:CAS:528: DyaK2sXpsVKqtQ%3D%3D 9015364 (Pubitemid 27225577)
46. M Kracht J Saklatvala 2002 Transcriptional and post-transcriptional control of gene expression in inflammation Cytokine 20 3 91 106 10.1006/cyto.2002.0895 1:CAS:528:DC%2BD38XoslGmsb8%3D 12453467 (Pubitemid 35370689)
47. AF Mendes AP Carvalho MM Caramona MC Lopes 2002 Role of nitric oxide in the activation of NF-kappaB, AP-1 and NOS II expression in articular chondrocytes Inflamm Res 51 369 75 10.1007/PL00000317 1:CAS:528: DC%2BD38Xlt1KksLk%3D 12146729
48. NL Malinin MP Boldin AV Kovalenko D Wallach 1997 MAP3K-related kinase involved in NF-kappaB induction by TNF, CD95 and IL-1 Nature 385 6616 540 4 10.1038/385540a0 1:CAS:528:DyaK2sXhtFCgtbo%3D 9020361 (Pubitemid 27074673)
49. Z Cao WJ Henzel X Gao 1996 IRAK: a kinase associated with the interleukin-1 receptor Science 271 5252 1128 31 10.1126/science.271.5252.1128 1:CAS:528:DyaK28Xht1Gktr8%3D 8599092
50. TT Chowdhury DM Salter DL Bader DA Lee 2004 Integrin-mediated mechanotransduction processes in TGFβ stimulated monolayer-expanded chondrocytes Biochem Biophys Res Commun 318 873 81 10.1016/j.bbrc.2004.04.107 1:CAS:528:DC%2BD2cXktV2mtLY%3D 15147953 (Pubitemid 38625855)
51. TT Chowdhury RN Appleby DM Salter DL Bader DA Lee 2006 Integrin-mediated mechanotransduction in IL-1β stimulated bovine chondrocytes cultured in agarose constructs Biomech Model Mechanobiol 5 2/3 192 201 10.1007/s10237-006- 0032-3 1:STN:280:DC%2BD283otVGhsw%3D%3D 16544161 (Pubitemid 43787102)
52. SJ Millward-Sadler NS Khan MG Bracher MO Wright DM Salter 2006 Roles for the interleukin-4 receptor and associated JAK/STAT proteins in human articular chondrocyte mechanotransduction Osteoarthr Cartil 14 10 991 1001 10.1016/j.joca.2006.03.013 1:STN:280:DC%2BD28rjvVyisg%3D%3D 16682236 (Pubitemid 44309416)
53. Salter DM, Millward-Sadler SJ, Nuki G, Wright MO. Integrin-interleukin-4 mechanotransduction pathways in human chondrocytes. Clin Orthop Relat Res. 2001;391(Suppl):S49-60.
54. TL Vincent CJ McLean LE Full D Peston J Saklatvala 2007 FGF-2 is bound to perlecan in the pericellular matrix of articular cartilage, where it acts as a chondrocyte mechanotransducer Osteoarthr Cartil 15 7 752 63 10.1016/j.joca.2007. 01.021 1:STN:280:DC%2BD2szlsFenuw%3D%3D 17368052 (Pubitemid 46888165)
55. 2+ selectively suppresses IL-1-induced NO production by reducing iNOS mRNA stability J Cell Biol 129 6 1651 7 10.1083/jcb.129.6.1651 1:CAS:528:DyaK2MXmt1ant7w%3D 7540612
56. TT Chowdhury MM Knight 2006 Purinergic pathway suppresses the release of NO and stimulates proteoglycan synthesis in chondrocyte/agarose constructs subjected to dynamic compression J Cell Phys 209 3 845 53 10.1002/jcp.20768 1:CAS:528:DC%2BD28XhtFOktLvE (Pubitemid 44665706)
57. SJ Millward-Sadler MO Wright PW Flatman DM Salter 2004 ATP in the mechanotransduction pathway of normal human chondrocytes Biorheology 41 567 75 1:CAS:528:DC%2BD2cXlvF2iu7o%3D 15299287