A versatile in vivo system for directed dissection of gene expression patterns

Nature Methods

Volumn 8, Issue 3, 2011, Pages 231-237

  Gohl, Daryl M ^a   Silies, Marion A ^a   Gao, Xiaojing J ^a   Bhalerao, Sheetal ^a   Luongo, Francisco J ^a   Lin, Chun Chieh ^b   Potter, Christopher J ^b   Clandinin, Thomas R ^a  

^a STANFORD UNIVERSITY   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LEXA PROTEIN; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR GAL4; TRANSCRIPTION FACTOR GAL80; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; DROSOPHILA MELANOGASTER; ENHANCER REGION; FEMALE; GENE EXPRESSION; GENE SEQUENCE; GENETIC ANALYSIS; GENETIC MANIPULATION; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; INTEGRASE SWAPPABLE IN VIVO TARGETING ELEMENT SYSTEM; MALE; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; TRANSPOSON;

ANIMALS; BACTERIAL PROTEINS; BASE SEQUENCE; DNA-BINDING PROTEINS; DROSOPHILA MELANOGASTER; ENHANCER ELEMENTS, GENETIC; GENE EXPRESSION; GENE EXPRESSION PROFILING; MOLECULAR SEQUENCE DATA; RECOMBINATION, GENETIC; REPRESSOR PROTEINS; SACCHAROMYCES CEREVISIAE PROTEINS; SERINE ENDOPEPTIDASES; TRANSCRIPTION FACTORS;

DROSOPHILA MELANOGASTER;

EID: 79952188287     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth.1561     Document Type: Article

References (47)

1. Brand, A.H. & Perrimon, N. Targeted gene expression as a means of altering cell fates and generating dominant phenotypes. Development 118, 401-415 (1993) (Pubitemid 23214028)
2. O'Kane, C.J. & Gehring, W.J. Detection in situ of genomic regulatory elements in Drosophila. Proc. Natl. Acad. Sci. USA 84, 9123-9127 (1987)
3. Ejsmont, R.K., Sarov, M., Winkler, S., Lipinski, K.A. & Tomancak, P. A toolkit for high-throughput, cross-species gene engineering in Drosophila. Nat. Methods 6, 435-437 (2009)
4. Pfeiffer, B.D. et al. Tools for neuroanatomy and neurogenetics in Drosophila. Proc. Natl. Acad. Sci. USA 105, 9715-9720 (2008) (Pubitemid 352031366)
5. Venken, K.J. et al. Versatile P[acman] BAC libraries for transgenesis studies in Drosophila melanogaster. Nat. Methods 6, 431-434 (2009)
6. Luo, L., Callaway, E.M. & Svoboda, K. Genetic dissection of neural circuits. Neuron 57, 634-660 (2008) (Pubitemid 351335341)
7. Korzh, V. Transposons as tools for enhancer trap screens in vertebrates. Genome Biol. 8 (Suppl. 1), S8 (2007)
8. Stanford, W.L., Cohn, J.B. & Cordes, S.P. Gene-trap mutagenesis: past, present and beyond. Nat. Rev. Genet. 2, 756-768 (2001) (Pubitemid 33685573)
9. Bellen, H.J. Ten years of enhancer detection: lessons from the fly. Plant Cell 11, 2271-2281 (1999) (Pubitemid 30031374)
10. Lai, S.L. & Lee, T. Genetic mosaic with dual binary transcriptional systems in Drosophila. Nat. Neurosci. 9, 703-709 (2006) (Pubitemid 44012270)
11. Potter, C.J., Tasic, B., Russler, E.V., Liang, L. & Luo, L. The Q system: a repressible binary system for transgene expression, lineage tracing, and mosaic analysis. Cell 141, 536-548 (2010)
12. Suster, M.L., Seugnet, L., Bate, M. & Sokolowski, M.B. Refining GAL4-driven transgene expression in Drosophila with a GAL80 enhancer-trap. Genesis 39, 240-245 (2004) (Pubitemid 39201545)
13. Bohm, R.A. et al. A genetic mosaic approach for neural circuit mapping in Drosophila. Proc. Natl. Acad. Sci. USA 107, 16378-16383 (2010)
14. Stockinger, P., Kvitsiani, D., Rotkopf, S., Tirian, L. & Dickson, B.J. Neural circuitry that governs Drosophila male courtship behavior. Cell 121, 795-807 (2005) (Pubitemid 40797601)
15. Gordon, M.D. & Scott, K. Motor control in a Drosophila taste circuit. Neuron 61, 373-384 (2009)
16. Luan, H., Peabody, N.C., Vinson, C.R. & White, B.H. Refined spatial manipulation of neuronal function by combinatorial restriction of transgene expression. Neuron 52, 425-436 (2006) (Pubitemid 44667428)
17. Lee, T. & Luo, L. Mosaic analysis with a repressible cell marker for studies of gene function in neuronal morphogenesis. Neuron 22, 451-461 (1999) (Pubitemid 29159845)
18. Bateman, J.R., Lee, A.M. & Wu, C.T. Site-specific transformation of Drosophila via phiC31 integrase-mediated cassette exchange. Genetics 173, 769-777 (2006) (Pubitemid 43945895)
19. Bischof, J., Maeda, R.K., Hediger, M., Karch, F. & Basler, K. An optimized transgenesis system for Drosophila using germ-line-specific phiC31 integrases. Proc. Natl. Acad. Sci. USA 104, 3312-3317 (2007) (Pubitemid 46364156)
20. Golic, M.M., Rong, Y.S., Petersen, R.B., Lindquist, S.L. & Golic, K.G. FLP-mediated DNA mobilization to specific target sites in Drosophila chromosomes. Nucleic Acids Res. 25, 3665-3671 (1997) (Pubitemid 27390186)
21. Horn, C. & Handler, A.M. Site-specific genomic targeting in Drosophila. Proc. Natl. Acad. Sci. USA 102, 12483-12488 (2005) (Pubitemid 41266385)
22. Oberstein, A., Pare, A., Kaplan, L. & Small, S. Site-specific transgenesis by Cre-mediated recombination in Drosophila. Nat. Methods 2, 583-585 (2005) (Pubitemid 41086848)
23. Schlake, T. & Bode, J. Use of mutated FLP recognition target (FRT) sites for the exchange of expression cassettes at defined chromosomal loci. Biochemistry 33, 12746-12751 (1994) (Pubitemid 24352862)
24. Yagi, R., Mayer, F. & Basler, K. Refined LexA transactivators and their use in combination with the Drosophila Gal4 system. Proc. Natl. Acad. Sci. USA 107, 16166-16171 (2010)
25. Groth, A.C., Fish, M., Nusse, R. & Calos, M.P. Construction of transgenic Drosophila by using the site-specific integrase from phage phiC31. Genetics 166, 1775-1782 (2004) (Pubitemid 38621059)
26. Thorpe, H.M. & Smith, M.C. In vitro site-specific integration of bacteriophage DNA catalyzed by a recombinase of the resolvase/invertase family. Proc. Natl. Acad. Sci. USA 95, 5505-5510 (1998)
27. Woltjen, K. et al. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature 458, 766-770 (2009)
28. Horn, C., Offen, N., Nystedt, S., Hacker, U. & Wimmer, E.A. piggyBac-based insertional mutagenesis and enhancer detection as a tool for functional insect genomics. Genetics 163, 647-661 (2003) (Pubitemid 36314547)
29. Rad, R. et al. PiggyBac transposon mutagenesis: a tool for cancer gene discovery in mice. Science 330, 1104-1107 (2010)
30. Li, X. et al. piggyBac internal sequences are necessary for efficient transformation of target genomes. Insect Mol. Biol. 14, 17-30 (2005) (Pubitemid 40218081)
31. Thibault, S.T. et al. A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac. Nat. Genet. 36, 283-287 (2004) (Pubitemid 38282754)
32. Siegal, M.L. & Hartl, D.L. Transgene coplacement and high efficiency site-specific recombination with the Cre/loxP system in Drosophila. Genetics 144, 715-726 (1996) (Pubitemid 26325862)
33. Maggert, K.A., Gong, W.J. & Golic, K.G. Methods for homologous recombination in Drosophila. Methods Mol. Biol. 420, 155-174 (2008)
34. Gao, S. et al. The neural substrate of spectral preference in Drosophila. Neuron 60, 328-342 (2008)
35. Newsome, T.P., Asling, B. & Dickson, B.J. Analysis of Drosophila photoreceptor axon guidance in eye-specific mosaics. Development 127, 851-860 (2000) (Pubitemid 30129031)
36. Venken, K.J., He, Y., Hoskins, R.A. & Bellen, H.J. P[acman]: a BAC transgenic platform for targeted insertion of large DNA fragments in D. melanogaster. Science 314, 1747-1751 (2006) (Pubitemid 44969080)
37. Malla, S., Dafhnis-Calas, F., Brookfield, J.F., Smith, M.C. & Brown, W.R. Rearranging the centromere of the human Y chromosome with phiC31 integrase. Nucleic Acids Res. 33, 6101-6113 (2005) (Pubitemid 41742549)
38. Pfeiffer, B.D. et al. Refinement of tools for targeted gene expression in Drosophila. Genetics 186, 735-755 (2010)
39. Awasaki, T., Lai, S.L., Ito, K. & Lee, T. Organization and postembryonic development of glial cells in the adult central brain of Drosophila. J. Neurosci. 28, 13742-13753 (2008).
40. Gohl, D., Muller, M., Pirrotta, V., Affolter, M. & Schedl, P. Enhancer blocking and transvection at the Drosophila apterous locus. Genetics 178, 127-143 (2008) (Pubitemid 351398736)
41. Schuldiner, O. et al. piggyBac-based mosaic screen identifies a postmitotic function for cohesin in regulating developmental axon pruning. Dev. Cell 14, 227-238 (2008) (Pubitemid 351189171)
42. Hagstrom, K., Muller, M. & Schedl, P. Fab-7 functions as a chromatin domain boundary to ensure proper segment specification by the Drosophila bithorax complex. Genes Dev. 10, 3202-3215 (1996) (Pubitemid 27020547)
43. Rubin, G.M. & Spradling, A.C. Genetic transformation of Drosophila with transposable element vectors. Science 218, 348-353 (1982) (Pubitemid 12037603)
44. Potter, C.J. & Luo, L. Splinkerette PCR for mapping transposable elements in Drosophila. PLoS ONE 5, e10168 (2010)
45. Ochman, H., Gerber, A.S. & Hartl, D.L. Genetic applications of an inverse polymerase chain reaction. Genetics 120, 621-623 (1988) (Pubitemid 18260496)
46. Tweedie, S. et al. FlyBase: enhancing Drosophila Gene Ontology annotations. Nucleic Acids Res. 37, D555-D559 (2009)
47. Wagh, D.A. et al. Bruchpilot, a protein with homology to ELKS/CAST, is required for structural integrity and function of synaptic active zones in Drosophila. Neuron 49, 833-844 (2006). (Pubitemid 43376081)