A complementary transposon tool kit for Drosophila melanogaster using P and piggyBac

Nature Genetics

Volumn 36, Issue 3, 2004, Pages 283-287

  Thibault, Stephen T ^a   Singer, Matthew A ^a,b   Miyazaki, Wesley Y ^a   Milash, Brett ^a,b,c   Dompe, Nicholas A ^a   Singh, Carol M ^a,b,d   Buchholz, Ross ^a   Demsky, Madelyn ^a   Fawcett, Robert ^a   Francis Lang, Helen L ^a   Ryner, Lisa ^a   Cheung, Lai Man ^a   Chong, Angela ^a   Erickson, Cathy ^a   Fisher, William W ^a   Greer, Kimberly ^a   Hartouni, Stephanie R ^a   Howie, Elizabeth ^a   Jakkula, Lakshmi ^a   Joo, Daniel ^a,b,e   Killpack, Keith ^a   Laufer, Alex ^a,b,f   Mazzotta, Julie ^a   Smith, Ronald D ^a   Stevens, Lynn M ^a   Stuber, Christiana ^a   Tan, Lory R ^a   Ventura, Richard ^a   Woo, Alesa ^a   Zakrajsek, Irena ^a,b,g   Zhao, Lora ^a   Chen, Feng ^a   Swimmer, Candace ^a   Kopczynski, Casey ^a,b,h   Duyk, Geoffrey ^a   Winberg, Margaret L ^a   Margolis, Jonathan ^a   more..

^a EXELIXIS INC   (United States)

^b Nippon Chemi Con   (Japan)

^c UNIVERSITY OF UTAH   (United States)

^d EnVivo Pharmaceuticals   (United States)

^e Metabolex   (United States)

^f EVOTEC AG   (Germany)

^g GENENTECH INC   (United States)

^h Ercole Biotech   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ARTICLE; DROSOPHILA MELANOGASTER; EXCISION REPAIR; GENE DISRUPTION; GENE INSERTION; GENE LOCATION; GENE SEQUENCE; GENETIC COMPLEMENTATION; KNOCKOUT GENE; LEPIDOPTERA; MOLECULAR GENETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; P GENE; PHENOTYPE; PIGGYBAC GENE; PRIORITY JOURNAL; RANDOMIZATION; TRANSPOSON;

ANIMALS; DNA TRANSPOSABLE ELEMENTS; DROSOPHILA MELANOGASTER; GENES, INSECT; MUTAGENESIS, INSERTIONAL;

ARACHNIDA; DROSOPHILA MELANOGASTER; HEXAPODA; LEPIDOPTERA; MELANOGASTER;

EID: 10744231523     PISSN: 10614036     EISSN: None     Source Type: Journal    
DOI: 10.1038/ng1314     Document Type: Article

References (26)

1. Adams, M.D. & Sekelsky, J.J. From sequence to phenotype: reverse genetics in Drosophila melanogaster. Nat. Rev. Genet. 3, 189-198 (2002).
2. Spradling, A.C. et al. The BDGP gene disruption project: single P element insertions mutating 25% of vital Drosophila genes. Proc. Natl. Acad. Sci. USA 153, 135-177 (1999).
3. Adams, M.D. et al. The Genome Sequence of Drosophila melanogaster. Science 287, 2185-2195 (2000).
4. Handler, A.M. & Harrell, R.A.I. Germline transformation of Drosophila melanogaster with the piggyBac transposon vector. Insect Mol. Biol. 8, 449-457 (1999).
5. Handler, A.M. A current perspective on insect gene transformation. Insect Biochem. Mol. Biol. 31, 111-128 (2001).
6. Horn, C., Offen, N., Nystedt, S., Häcker, U. & Wimmer, E.A. piggyBac-based insertional mutagenesis and enhancer detection asa tool for functional insect genomics. Genetics 163, 647-661 (2003).
7. Hacker, U., Nystedt, S., Barmchi, M.P., Horn, C. & Wimmer, E.A. piggyBac-based insertional mutagenesis in the presence of stably integrated P elements in Drosophila. Proc. Natl. Acad. Sci. USA 100, 7720-7725 (2003).
8. Zhang, P. & Spradling, A.C. Efficient and dispersed local P element transposition from Drosophila females. Genetics 133, 361-373 (1993).
9. Ashburner, M.A. Drosophila: a Laboratory Handbook (Cold Spring Harbor Laboratory Press, New York, 1989).
10. Cooley, L., Kelley, R. & Spradling, A.C. Insertional mutagenesis of the Drosophila genome with single Pelements. Science 239, 1121-1128 (1988).
11. Rørth, P. A modular misexpression screen in Drosophila detecting tissue-specific phenotypes. Proc. Natl. Acad. Sci. USA 93, 12418-12422 (1996).
12. Spradling, A.C. et al. Gene disruptions using P transposable elements: an integral component of the Drosophila genome project. Proc. Natl. Acad. Sci. USA 92, 10824-10830 (1995).
13. Horowitz, H. & Berg, C.A. Aberrant splicing and transcriptional termination caused by P element insertion into the intron of a Drosophila gene. Genetics 139, 327-335 (1995).
14. Goodwin, S.F. et al. Aberrant splicing and altered spatial expression patterns in fruit less mutants of Drosophila melanogaster. Genetics 154, 725-745 (2000).
15. Cho, N. et al. Developmental control of blood cell migration by the Drosophila VEGF pathway. Cell 108, 865-876 (2002).
16. Gottar, M. et al. The Drosophila immune response against Gram-negative bacteria is mediated by a peptidoglycan recognition protein. Nature 416, 640-644 (2002).
17. Pirrotta, V. Vectors for P mediated transformation in Drosophila. in Vectors: A Survey of Molecular Cloning Vectors and Their Uses (eds. R.L. Rodriquez & D.T. Denhart) 437-456 (Butterworth, Boston, 1988).
18. Sadowski, P. The Flp recombinases of the 2-microns plasmid of Saccharomyces cerevisiae. Prog. Nucleic Acid Res. Mol. Biol. 51, 53-91 (1995).
19. Kim, Y.J., Zuo, P., Manley, J.L. & Baker, B.S. The Drosophila RNA-binding protein RBP1 is localized to transcriptionally active sites of chromosomes and shows a functional similarity to human splicing factor ASF/SF2. Genes Dev. 6, 2569-2579 (1992).
20. Roseman, R.R. et al. A P element containing suppressor of Hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster. Genetics 141, 1061-1064 (1995).
21. Parks, A.L. et al. Systematic generation of high-resolution deletion coverage of the Drosophila melanogaster genome. Nat. Genet. advance online publication, 22 February 2004 (doi: 10.1038/ng1312).
22. Golic, K.G. & Lindquist, S. The FLP recombinase of yeast catalyzes site-specific recombination in the Drosophila genome. Cell 59, 499-509 (1989).
23. Theurkauf, W.E., Baum, H., Bo, J. & Wensink, P.C. Tissue-specific and constitutive α-tubulin genes of Drosophila melanogaster code for structurally distinct proteins. Proc. Natl. Acad. Sci. USA 83, 8477-8481 (1986).
24. Serano, T.L. & Cohen, R.S. A small predicted stem-loop structure mediates oocyte localization of Drosophila KID mRNA. Development 121, 3809-3818 (1995).
25. Rørth, P. Gal4 in the Drosophila female germline. Mech. Dev. 78, 113-118 (1998).
26. Robertson, H.M. et al. A stable genomic source of P element transposase in Drosophila melanogaster. Genetics 118, 461-470 (1988).