Screening of genetic parameters for soluble protein expression in Escherichia coli

Protein Expression and Purification

Volumn 77, Issue 1, 2011, Pages 104-111

  Vernet, Erik ^a   Kotzsch, Alexander ^a   Voldborg, Bjørn ^a   Sundström, Michael ^a  

^a University of Copenhagen   (Denmark)

Author keywords

DsbA; Escherichia coli; Ligation independent cloning; OsmY; pelB; Recombinant protein expression

Indexed keywords

DISULFIDE; ESCHERICHIA COLI PROTEIN; HYBRID PROTEIN; INTERLEUKIN DERIVATIVE; SIGNAL PEPTIDE;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; CODON; COMPARATIVE STUDY; ESCHERICHIA COLI; GENETICS; HUMAN; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; REPRODUCIBILITY; SOLUBILITY; SURFACE PLASMON RESONANCE;

CLONING, MOLECULAR; CODON; DISULFIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HUMANS; INTERCELLULAR SIGNALING PEPTIDES AND PROTEINS; INTERLEUKINS; PLASMIDS; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; REPRODUCIBILITY OF RESULTS; SOLUBILITY; SURFACE PLASMON RESONANCE;

ESCHERICHIA COLI;

EID: 79951943981     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.11.016     Document Type: Article

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