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79951691094
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note
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Rh-101, 8-(2-carboxyphenyl)-2,3,5,6,11,12,14,15-octahydro-1 H,4 H,10 H,13 H -diquinolizino[9,9 a,1- bc:9′,9 a ′,1- hi ]xanthylium perchlorate; CV, 5,9-diaminobenzo[ a ]phenoxazonium perchlorate; Ox-170, 9-ethylamino-5-ethylimino-10-methyl-5 H -benzo[ a ]phenoxazonium perchlorate; Ox-1, 3-diethylamino-7-diethyliminophenoxazonium perchlorate; Cc, 1,1′-diethyl-4,4′-carbocyanine iodide; HITCI, 1,1′,3,3, 3′,3′-hexamethylindotricarbocyanine iodide; IR-140, 3,3′-diethyl-5,5-dichloro-3,5-ethylene-4-(diphenylamino)-2, 2′-indotricarbocyanine perchlorate; IR-125, 1,1′- di(butylenesulfonate)-3,3,3′,3′-tetramethyl-4,4′,5, 5′-dibenzo-2,2′-indotricarbocyanine sodium; C-102, 2,3,5,6,-1 H,4 H -tetrahydro-8-methylquinolizino-(9,9 a,1- gh)coumarin; C-153, 2,3,5,6,-1 H,4 H -tetrahydro-8-trifluormethylquinolizino-(9,9 a,1- gh)coumarin; DCM, 4-dicyanomethylene-2-methyl-6-(p -dimethylaminostyryl)-4 H -pyran.
-
-
-
-
44
-
-
79951698062
-
-
note
-
HEDITCP, 1,1′,3,3,3′,3′-hexamethyl-3,5-ethylene-4- (dimethylamino)-2,2′-indotricarbocyanine perchlorate; HPDITCP, 1,1′,3,3,3′,3′-hexamethyl-3,5-propylene-4-(dimethylamino)-2, 2′-indotricarbocyanine perchlorate.
-
-
-
-
46
-
-
79951683749
-
-
note
-
ONITCP, 1,1′,3,3,3′,3′(3,3′)-octamethyl-3,5- neopentylene-2,2′-indotetracarbocyanine perchlorate; ODNITCP, 1,1′,3,3,3′,3′(3,3′)-octamethyl-4,4′,5, 5′-dibenzo-3,5-neopentylene-2,2′-indotetracarbocyanine perchlorate.
-
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47
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ex was verified for these dyes across the wavelength range of their first absorption bands.
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With regard to the method of degassing, the performance of Ar deoxygenation proved to be similarly effective to the frequently employed freeze-pump-thaw technique while offering more straightforward handling.
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79951684525
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For dependences, see the respective entries for ethanol in ref 48.
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69
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79951686635
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note
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When lowering the temperature and especially when freezing the solvent, significant changes in viscosity occur and give rise to polarization effects, rendering the use of defined polarization conditions in excitation and emission mandatory. Furthermore, it is important to use optically dilute solutions because the absorption and emission bands are not only narrowed upon reduction of the temperature, but the Stokes shift is commonly also decreased, rendering reabsorption errors more significant at low temperatures.
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The weaker the fluorescence of a compound, the higher is usually the relative uncertainty introduced by the experimental standard deviation; see section II.2.3e, Supporting Information.
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83
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79951709810
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Due to its considerably low fluorescence quantum yield and high anisotropy, Cc is not included here.
-
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84
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79951702214
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note
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The fluorescence emission of C-102, C-153, DCM, Rh-101, CV, Ox-170, Ox-1, and HEDITCP does not depend on the excitation wavelength within the specified excitation range. For HITCI, IR-125, IR-140, ONITCP, and ODNITCP, the match of absorption and fluorescence excitation spectra could not be verified because of the spectral limitations of the excitation channel of the instrument employed. As reported in ref 43, absorption and fluorescence excitation spectra show a slight mismatch above ca. 680 nm for HPDITCP. Below 670 nm, the fluorescence quantum yield is independent of excitation wavelength in the specified range.
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