Stabilization of the single-chain fragment variable by an interdomain disulfide bond and its effect on antibody affinity

International Journal of Molecular Sciences

Volumn 12, Issue 1, 2011, Pages 1-11

  Zhao, Jian Xin ^a   Yang, Lian ^a   Gu, Zhen Nan ^b   Chen, Hai Qin ^a   Tian, Feng Wei ^a   Chen, Yong Quan ^b   Zhang, Hao ^a   Chen, Wei ^a  

^a JIANGNAN UNIVERSITY   (China)

^b WAKE FOREST SCHOOL OF MEDICINE   (United States)

Author keywords

Affinity; Aflatoxin; Disulfide bond; Single chain fragment variable; Stability

Indexed keywords

AFLATOXIN B1; AFLATOXIN B1 SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; GUANIDINE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; UNCLASSIFIED DRUG; DISULFIDE;

ANTIBODY AFFINITY; ARTICLE; BINDING AFFINITY; DISULFIDE BOND; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; IC 50; MOLECULAR DOCKING; MOLECULAR MODEL; MUTANT; NONHUMAN; PROTEIN AGGREGATION; PROTEIN STABILITY; SEQUENCE HOMOLOGY; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETICS; PROTEIN TERTIARY STRUCTURE;

ANTIBODY AFFINITY; DISULFIDES; ENZYME-LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; MODELS, MOLECULAR; PROTEIN STABILITY; PROTEIN STRUCTURE, TERTIARY; SINGLE-CHAIN ANTIBODIES;

EID: 79251610615     PISSN: None     EISSN: 14220067     Source Type: Journal    
DOI: 10.3390/ijms12010001     Document Type: Article

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