Expression, purification and kinetic characterization of His-tagged glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi

Protein Expression and Purification

Volumn 76, Issue 2, 2011, Pages 190-196

  Cheleski, Juliana ^a   Freitas, Renato F ^a   Wiggers, Helton José ^a,b   Rocha, Josmar R ^a   De Araújo, Ana Paula Ulian ^a   Montanari, Carlos A ^a,b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

Author keywords

Chagas' disease; Escherichia coli expression; Experimental design; Isothermal titration calorimetry; Kinetic parameters; Trypanosoma cruzi Glyceraldehyde 3 phosphate dehydrogenase

Indexed keywords

GLYCERALDEHYDE 3 PHOSPHATE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HYBRID PROTEIN; PROTOZOAL PROTEIN;

AFFINITY CHROMATOGRAPHY; ARTICLE; CALORIMETRY; CHEMISTRY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; PH; TEMPERATURE; TRYPANOSOMA CRUZI;

CALORIMETRY; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; ESCHERICHIA COLI; GLYCERALDEHYDE 3-PHOSPHATE; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE (PHOSPHORYLATING); HYDROGEN-ION CONCENTRATION; PROTOZOAN PROTEINS; RECOMBINANT FUSION PROTEINS; TEMPERATURE; TRYPANOSOMA CRUZI;

ESCHERICHIA COLI; MASTIGOPHORA (FLAGELLATES); PROTOZOA; TRYPANOSOMA; TRYPANOSOMA CRUZI;

EID: 79151481852     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.11.013     Document Type: Article

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