Kinetic mechanism and catalysis of Trypanosoma cruzi dihydroorotate dehydrogenase enzyme evaluated by isothermal titration calorimetry

Analytical Biochemistry

Volumn 399, Issue 1, 2010, Pages 13-22

  Cheleski, Juliana ^a   Wiggers, Helton José ^b   Citadini, Ana Paula ^a   da Costa Filho, Antônio José ^a   Nonato, Maria Cristina ^a   Montanari, Carlos Alberto ^a,b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b FEDERAL UNIVERSITY OF SÃO CARLOS   (Brazil)

Author keywords

Activation thermodynamic parameters; Chagas disease; Dihydroorotate dehydrogenase; Enzyme kinetics; Trypanosoma cruzi

Indexed keywords

ACTIVATION ANALYSIS; ARRHENIUS PLOTS; CALORIMETERS; CALORIMETRY; CATALYSIS; CHEMICAL ACTIVATION; DIMETHYL SULFOXIDE; ENTROPY; ENZYME KINETICS; FREE ENERGY; GIBBS FREE ENERGY; ISOTHERMS; KINETICS; QUANTUM CHEMISTRY; TITRATION;

ACTIVATION ENTROPIES; CATALYTIC MECHANISMS; CHAGAS DISEASE; CONCOMITANT REDUCTION; DIHYDROOROTATE DEHYDROGENASE; ISOTHERMAL TITRATION CALORIMETRY; THERMODYNAMIC PARAMETER; TRYPANOSOMA CRUZI;

SUBSTRATES;

CARBOXYLIC ACID DERIVATIVE; DIHYDROOROTATE DEHYDROGENASE; DIHYDROOROTIC ACID; DIMETHYL SULFOXIDE; FUMARIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CATALYSIS; CONTROLLED STUDY; ENTROPY; ENZYME ASSAY; ENZYME KINETICS; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; ISOTHERMAL TITRATION CALORIMETRY; MICHAELIS CONSTANT; MICHAELIS MENTEN KINETICS; NONHUMAN; PRIORITY JOURNAL; THERMODYNAMICS; TRYPANOSOMA CRUZI;

TRYPANOSOMA CRUZI;

EID: 77649180083     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.11.018     Document Type: Article

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