Effects of organic solvents on the enzyme activity of Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase in calorimetric assays

Analytical Biochemistry

Volumn 370, Issue 1, 2007, Pages 107-114

  Wiggers, H J ^a   Cheleski, J ^a   Zottis, A ^a   Oliva, G ^a   Andricopulo, A D ^a   Montanari, C A ^a  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

Author keywords

Chagas disease; Enzyme kinetics; Isothermal titration calorimetry; Organic solvents

Indexed keywords

CALORIMETERS; CALORIMETRY; DIAGNOSIS; DIMETHYL SULFOXIDE; ENZYME ACTIVITY; ENZYME KINETICS; ISOTHERMS; KINETICS; TITRATION;

BEHAVIORAL PERFORMANCE; CHAGAS DISEASE; DIMETHYL SULFOXIDE (DMSO); ENZYME-SUBSTRATE COMPLEXES; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE; ISOTHERMAL TITRATION CALORIMETRY; MACROMOLECULAR TARGETS; SPECTROPHOTOMETRIC ASSAYS;

ORGANIC SOLVENTS;

DIMETHYL SULFOXIDE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; METHANOL;

ARTICLE; CATALYSIS; CATALYST; ENZYME ACTIVITY; ISOTHERMAL TITRATION CALORIMETRY; MICHAELIS MENTEN KINETICS; NONHUMAN; PRIORITY JOURNAL; REACTION ANALYSIS; REPRODUCIBILITY; SPECTROPHOTOMETER; TRYPANOSOMA CRUZI;

TRYPANOSOMA CRUZI;

EID: 34548613757     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2007.06.042     Document Type: Article

References (26)

1. Ababou A., and Ladbury J.E. Survey of the year 2005: Literature on applications of isothermal titration calorimetry. J. Mol. Recognit. 20 (2007) 4-14
2. Di L., and Kerns E.H. Biological assay challenges from compound solubility: Strategies for bioassay optimization. Drug Discov. Today 11 (2006) 446-451
3. Molaei Rad A., Ghourchian H., Moosavi-Movahedi A.A., Hong J., and Nazari K. Spectrophotometric assay for horseradish peroxidase activity based on pyrocatechol-aniline coupling hydrogen donor. Anal. Biochem. 362 (2007) 38-43
4. Cardoso C.L., Lima V.V., Zottis A., Oliva G., Andricopulo A.D., Wainer I.W., Moaddel R., and Cass Q.B. Development and characterization of an immobilized enzyme reactor (IMER) based on human glyceraldehyde-3-phosphate dehydrogenase for on-line enzymatic studies. J. Chromatogr. A 1120 (2006) 151-157
5. Martins S., Karmali A., and Serralheiro M.L. Kinetic properties of wild-type and altered recombinant amidases by the use of ion-selective electrode assay method. Anal. Biochem. 355 (2006) 232-239
6. Olsen S.N. Applications of isothermal titration calorimetry to measure enzyme kinetics and activity in complex solutions. Thermochim. Acta 448 (2006) 12-18
7. Michaelis L., and Menten M.L. Kinetics of invertase action. Biochemistry 49 (1913) 333-369
8. Coura J.R., and De Castro S.L. A critical review on Chagas disease chemotherapy. Mem. Inst. Oswaldo Cruz 97 (2002) 3-24
9. Engel J.C., Cazzulo B.M.F., Stoppani A.O.M., Cannata J.J.B., and Cazzulo J.J. Aerobic glucose fermentation by Trypanosoma cruzi axenic culture amastigote-like forms during growth and differentiation to epimastigotes. Mol. Biochem. Parasit. 26 (1987) 1-10
10. Pavão F., Castilho M.S., Pupo M.T., Dias R.L., Correa A.G., Fernandes J.B., da Silva M.F., Mafezoli J., Vieira P.C., and Oliva G. Structure of Trypanosoma cruzi glycosomal glyceraldehydes-3-phosphate dehydrogenase complexed with chalepin, a natural product inhibitor, at 1.95 Å resolution. FEBS Lett. 520 (2002) 13-17
11. Ladame S., Castilho M.S., Silva C.H., Denier C., Hannaert V., Perie J., Oliva G., and Willson M. Crystal structure of Trypanosoma cruzi glyceraldehydes-3-phosphate dehydrogenase complexed with an analogue of 1,3-bisphospho-d-glyceric acid. Eur. J. Biochem. 270 (2003) 4574-4586
12. Weber P.C., and Salemme F.R. Applications of calorimetric methods to drug discovery and the study of protein interactions. Curr. Opin. Struct. Biol. 13 (2003) 115-121
13. Wiseman T., Williston S., Brandts J.F., and Lin L.N. Rapid measurement of binding constants and heats of binding using a new titration calorimeter. Anal. Biochem. 179 (1989) 131-137
14. Bianconi M.L. Calorimetric determination of thermodynamic parameters of reaction reveals different enthalpic compensations of the yeast hexokinase isozymes. J. Biol. Chem. 278 (2003) 18709-18713
15. Todd M.J., and Gomez J. Enzyme kinetics determined using calorimetry: A general assay for enzyme activity?. Anal. Biochem. 296 (2001) 179-187
16. Hannaert V., Opperdoes F.R., and Michels P.A.M. Glycosomal glyceraldehyde-3-phosphate dehydrogenase of Trypanosoma brucei and Trypanosoma cruzi: Expression in Escherichia coli, purification, and characterization of the enzymes. Protein Express. Purif. 6 (1995) 244-250
17. Souza D.H., Garratt R.C., Araújo A.P., Guimarães B.G., Jesus W.D., Michels P.A., Hannaert V., and Oliva G. Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase: Structure, catalytic mechanism, and targeted inhibitor design. FEBS Lett. 424 (1998) 131-135
18. Malhotrat O.P., and Bernhard S.A. Role of nicotinamide adenine dinucleotide as an effector in formation and reactions of acylglyceraldehyde-3-phosphate dehydrogenase. Biochemistry 20 (1981) 5529-5538
19. Byers L.D., She H.S., and Alayoff A. Interaction of phosphate analogues with glyceraldehydes-3-phosphate dehydrogenase. Biochemistry 18 (1979) 2471-2480
20. MicroCal, ITC Data Analysis in Origin, Tutorial Guide, Version 7.0, MicroCal, Northampton, MA, 2004.
21. Poduch E., Bello A.M., Tang S., Fujihashi M., Pai E.F., and Kotra L.P. Design of inhibitors of orotidine monophosphate decarboxylase using bioisosteric replacement and determination of inhibition kinetics. J. Med. Chem. 49 (2006) 4937-4945
22. Tjenberg A., Markova N., Griffiths W.J., and Hallén D. DMSO-related effects in protein characterization. J. Biomol. Screen. 11 (2006) 131-137
23. Goodman M., Listowsky I., Masuda Y., and Boardman F. Conformational aspects of polypeptides: VIII. Helical assignments via far ultraviolet absorption spectra and optical activity. Biopolymers 1 (1963) 33-42
24. Conio G., Patrone E., and Brighetti S. The effect of aliphatic alcohols on the helix-coil transition of poly-l-ornithine and poly-l-glutamic acid. J. Biol. Chem. 245 (1970) 3335-3340
25. Herskovits T., Gadebeku B., and Jaillet H. On the structural stability and solvent denaturation of proteins: I. Denaturation by the alcohols and glycols. J. Biol. Chem. 245 (1970) 2588-2598
26. Ababou A., and Ladbury J.E. Survey of the year 2004: Literature on applications of isothermal titration calorimetry. J. Mol. Recognit. 19 (2006) 79-89