An isothermal titration calorimetric method to determine the kinetic parameters of enzyme catalytic reaction by employing the product inhibition as probe

Analytical Biochemistry

Volumn 299, Issue 1, 2001, Pages 19-23

  Cai, Lifeng ^a   Cao, Aoneng ^a   Lai, Luhua ^a  

^a Peking University   (China)

Author keywords

CCMP; Enzyme; Inhibition; ITC; Kinetics; RNase A

Indexed keywords

CALORIMETRY; CATALYSIS; DISSOCIATION; ENZYME KINETICS; ENZYMES; ISOTHERMS; KINETIC PARAMETERS; MOLAR CONCENTRATION; PROBES; TITRATION;

CALORIMETRIC MEASUREMENTS; CALORIMETRIC METHODS; CATALYTIC HYDROLYSIS; CATALYTIC REACTIONS; CCMP; DISSOCIATION CONSTANT; ISOTHERMAL TITRATION; RNASE A;

ENZYME INHIBITION;

CYTIDINE 2',3' PHOSPHATE; CYTIDINE DERIVATIVE; RIBONUCLEASE A; UNCLASSIFIED DRUG;

ARTICLE; CALORIMETRY; CATALYSIS; CHEMICAL REACTION KINETICS; DISSOCIATION CONSTANT; ENZYME INHIBITION; HEAT; HYDROLYSIS; MICHAELIS MENTEN KINETICS; PRIORITY JOURNAL; REACTION ANALYSIS; TITRIMETRY;

EID: 0035577804     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1006/abio.2001.5397     Document Type: Article

References (17)

1. Rapid measurement of binding constants and heats of binding using a new titration calorimeter
2. Isothermal titration calorimetry
3. Isothermal titration microcalorimetry
4. The application of isothermal titration calorimetry to thermodynamics studied of biomolecular interaction
5. Theoretical aspects of isothermal titration calorimetry
6. Calorimetric evaluation of enzyme kinetic parameters
7. Enzyme activity determination on macromolecular substrates by isothermal titration calorimetry: Application to mesophilic and psychrophilic chitinases
8. Direct calorimetric analysis of the enzymatic activity of yeast cytochrome c oxidase
9. The active site and mechanism of action of bovine pancreatic ribonuclease. 3. The pH-dependence of the kinetic parameters for the hydrolysis of cytidine 2′,3′-phosphate
10. The active site and mechanism of action of bovine pancreatic ribonuclease. 4. The activity in inert organic solvents and alcohols
11. The active site and mechanism of action of bovine pancreatic ribonuclease. 5. The charge types at the active center
12. The active site and mechanism of action of bovine pancreatic ribonuclease. 6. Kinetic and spectrophotometric investigation of the interaction of the enzyme with inhibitors and p-nitrophenyl acetate
13. The active site and mechanism of action of bovine pancreatic ribonuclease. 7. The catalytic mechanism
14. Energetics of ribonuclease A catalysis. 1. pH, ionic strength, and solvent isotope dependence of the hydrolysis of cytidlne cyclic 2′,3′-phosphate
15. Energetics of ribonuclease A catalysis. 2. Nonenzymatic hydrolysis of cytidine cyclic 2′,3′-phosphate
16. Energetics of ribonuclease A catalysis. 3. Temperature dependence of the hydrolysis of cytidine cyclic 2′,3′-phosphate