Cloning and targeted disruption of two lipopolysaccharide biosynthesis genes, kdsA and waaG, of pseudomonas aeruginosa PAO1 by site-directed mutagenesis

Journal of Molecular Microbiology and Biotechnology

Volumn 19, Issue 4, 2011, Pages 169-179

  Perumal, Deepak ^a,b   Sakharkar, Kishore R ^b,d   Tang, Thean Hock ^d   Chow, Vincent T K ^c   Lim, Chu Sing ^b   Samal, Areejit ^e,f   Sugiura, Norio ^g   Sakharkar, Meena K ^a,b,g  

^a NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^b NANYANG TECHNOLOGICAL UNIVERSITY   (Singapore)

^c NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^d ADVANCED MEDICAL AND DENTAL INSTITUTE   (Malaysia)

^e MAX PLANCK INSTITUTE FOR MATHEMATICS IN THE SCIENCES   (Germany)

^f INRA Institut National de la Recherche Agronomique   (France)

^g UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Antibacterial drugs; Essential genes; Lipopolysaccharide biosynthesis; Plasmid insertion mutagenesis

Indexed keywords

2 DEHYDRO 3 DEOXYPHOSPHOOCTONATE ALDOLASE; AMPICILLIN; GLUCOSYLTRANSFERASE; KANAMYCIN; LIPOPOLYSACCHARIDE; TRANSALDOLASE; UNCLASSIFIED DRUG; URIDINE TRIPHOSPHATE GLUCOSE(HEPTOSYL)LIPOPOLYSACCHARIDE ALPHA 1,3 GLUCOSYLTRANSFERASE;

ANTIBIOTIC RESISTANCE; ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL SURVIVAL; CHROMOSOME MARKER; CONTROLLED STUDY; GENE DISRUPTION; GENE DUPLICATION; GENE INSERTION; GENE SYNTHESIS; GENE TARGETING; KDSA GENE; MARKER GENE; MOLECULAR CLONING; MOLECULAR GENETICS; NONHUMAN; PLASMID; PSEUDOMONAS AERUGINOSA; SITE DIRECTED MUTAGENESIS; VALIDATION STUDY; WAAG GENE;

ALDEHYDE-LYASES; ANTI-BACTERIAL AGENTS; CHROMOSOMES; CLONING, MOLECULAR; DRUG RESISTANCE, BACTERIAL; GENES, BACTERIAL; GLUCOSYLTRANSFERASES; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; PSEUDOMONAS AERUGINOSA;

BACTERIA (MICROORGANISMS); PSEUDOMONAS AERUGINOSA;

EID: 78751512046     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: 10.1159/000322157     Document Type: Article

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