Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesis

Journal of Bacteriology

Volumn 183, Issue 4, 2001, Pages 1259-1268

  Chalker, A F ^a   Minehart, H W ^a   Hughes, N J ^a   Koretke, K K ^a   Lonetto, M A ^a   Brinkman, K K ^a   Warren, P V ^a   Lupas, A ^a   Stanhope, M J ^a   Brown, J R ^a   Hoffman, P S ^a  

^a GLAXOSMITHKLINE   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ALLELE; ARTICLE; CONTROLLED STUDY; GENETIC ANALYSIS; GENETIC CONSERVATION; GENETIC SCREENING; GENOME; HELICOBACTER PYLORI; MUTAGENESIS; NONHUMAN; OPEN READING FRAME; PHYLOGENY; PRIORITY JOURNAL;

ALLELES; BASE SEQUENCE; CONSERVED SEQUENCE; EVOLUTION, MOLECULAR; GENES, BACTERIAL; GENES, ESSENTIAL; GENOME, BACTERIAL; HELICOBACTER PYLORI; MUTAGENESIS, INSERTIONAL; OPEN READING FRAMES; PHYLOGENY; SPECIES SPECIFICITY;

EID: 0035143428     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.183.4.1259-1268.2001     Document Type: Article

References (55)

1. Antibiotic resistance problems with Helicobacter pylori
2. Genomic-sequence comparison of two unrelated isolates of the human gastric pathogen Helicobacter pylori
3. Gapped BLAST and PSI-BLAST: A new generation of protein database search programs
4. An iron-regulated alkyl hydroperoxide reductase (AhpC) confers aerotolerance and oxidative stress resistance to the microaerophilic pathogen Campylobacter jejuni
5. Fructose-bisphosphatase-deficient mutants of mucoid Pseudomonas aeruginosa
6. Molecular characterization of two-component systems of Helicobacter pylori
7. The ferric uptake regulator (Fur) homologue of Helicobacter pylori: Functional analysis of the coding gene and controlled production of the recombinant protein in Escherichia coli
8. The complete genome sequence of Escherichia coli K-12
9. Cloning, sequencing, expression, purification and preliminary characterization of a type II dehydroquinase from Helicobacter pylori
10. How many membrane proteins are there?
11. Antibiotic discovery: Is it in the genes?
12. Complete genome sequence of the methanogenic archaeon, Methanococcus jannaschii
13. The Helicobacter pylori pyrB gene encoding aspartate carbamoyltransferase is essential for bacterial survival
14. cag, a pathogenicity island of Helicobacter pylori, encodes type I-specific and disease-associated virulence factors
15. Genetic characterization of Gram-positive homologs of the XerCD site-specific recombinases
16. TopPred II: An improved software for membrane protein structure predictions
17. Helicobacter pylori-selective antibacterials based on inhibition of pyrimidine biosynthesis
18. A model of evolutionary change in proteins
19. Helicobacter pylori physiology predicted from genomic comparison of two strains
20. Pseudomonas aeruginosa plasmids as suicide vectors in Escherichia coli: Resolution of genomic cointegrates through short regions of homology
21. Identifying nonpolar transbilayer helices in amino acid sequences of membrane proteins
22. Transmission of Helicobacter pylori
23. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd.
24. Mutational analysis of the vacA promoter provides insight into gene transcription in Helicobacter pylori
25. Rapid accumulation of intracellular 2-keto-3-deoxy-6-phosphogluconate in an Entner-Doudoroff aldolase mutant results in bacteriostasis
26. Identification and expression of the Bacillus subtilis fructose-1,6-bisphosphatase gene (fbp)
27. The yeast genome directory
28. Recombination between chromosomal IS200 elements supports frequent duplication formation in Salmonella typhimurium
29. Metabolic activities of metronidazole-sensitive and -resistant strains of Helicobacter pylori: Repression of pyruvate oxidoreductase and expression of isocitrate lyase activity correlate with resistance
30. Helicobacter pylori porCDAB and oorDABC genes encode distinct pyruvate: Flavodoxin and 2-oxoglutarate: Acceptor oxidoreductases which mediate electron transport to NADP
31. Another unusual type of citric acid cycle enzyme in Helicobacter pylori: The malate: Quinone oxidoreductase
32. Cloning of the fic-1 gene involved in cell filamentation induced by cyclic AMP and construction of a δfic Escherichia coli strain
33. Complete sequence and gene organization of the genome of a hyper-thermophilic archaebacterium, Pyrococcus horikoshii OT3
34. The complete genome sequence of the hyperthermophilic, sulphate-reducing archaeon Archaeoglobus fulgidus
35. The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
36. Metabolism and genetics of Helicobacter pylori: The genomic era
37. The Entner-Doudoroff pathway in Helicobacter pylori
38. Helicobacter pylori in peptic ulcer disease. NIH Consensus Development Panel on Helicobacter pylori in Peptic Ulcer Disease
39. TREEVIEW: An application to display phylogenetic trees on personal computers
40. The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences
41. PcrA is an essential DNA helicase of Bacillus subtilis fulfilling functions both in repair and rolling-circle replication
42. The tricarboxylic acid cycle of Helicobacter pylori
43. Ahpf can be dissected into two functional units: Tandem repeats of two thioredoxin-like folds in the N-terminus mediate electron transfer from the thioredoxin reductase-like C-terminus to AhpC
44. Complete genome sequence of Methanobacterium thermoautotrophicum δH: Functional analysis and comparative genomics
45. Disulfide bond formation in the Escherichia coli cytoplasm: An in vivo role reversal for the thioredoxins
46. Genetic analyses of the in vivo function of LolA, a periplasmic chaperone involved in the outer membrane localization of Escherichia coli lipoproteins
47. CLUSTALW: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
48. The complete genome sequence of the gastric pathogen Helicobacter pylori
49. Membrane protein structure prediction. Hydrophobicity analysis and the positive-inside rule
50. Transformation of Helicobacter pylori by chromosomal metronidazole resistance and by a plasmid with a selectable chloramphenicol resistance marker
51. Chloramphenicol resistance in Campylobacter coli: Nucleotide sequence, expression, and cloning vector construction
52. Isolation and characterization of pyrimidine auxotrophs, and molecular cloning of the pyre gene from the hyperthermophilic archeon Pyrococcus abyssi
53. Cloning and nucleotide sequence of the carboxynorspermidine decarboxylase gene from Vibrio alginolyticus