Identification of essential genes in Streptococcus pneumoniae by allelic replacement mutagenesis

Molecules and Cells

Volumn 19, Issue 3, 2005, Pages 365-374

  Song, Jae Hoon ^a,b   Ko, Kwan Soo ^a   Lee, Ji Young ^a   Baek, Jin Yang ^a   Oh, Won Sup ^b   Yoon, Ha Sik ^c   Jeong, Jin Yong ^d   Chun, Jongsik ^e  

^a Asia Pacific Foundation for Infectious Diseases (APFID)   (South Korea)

^b Sungkyunkwan University School of Medicine   (South Korea)

^c LG Chemical Ltd   (South Korea)

^d Asan Institute for Life Sciences   (South Korea)

^e Seoul National University   (South Korea)

Author keywords

Allelic Replacement Mutagenesis; Essential Genes; Genomics; New Antimicrobial agent; Pneumococci

Indexed keywords

ALLELE; AMINO ACID SEQUENCE; ARTICLE; BACILLUS SUBTILIS; BACTERIAL STRAIN; BACTERIAL VIRULENCE; ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; ESSENTIAL GENE; GENE TARGETING; MUTAGENESIS; ORTHOLOGY; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PNEUMONIAE; BACTERIAL GENE; BACTERIAL GENOME; GENE EXPRESSION REGULATION; GENETICS; METHODOLOGY; POLYMERASE CHAIN REACTION;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); ENTEROCOCCUS FAECALIS; ESCHERICHIA COLI; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PNEUMONIAE;

GENES, BACTERIAL; GENES, ESSENTIAL; GENOME, BACTERIAL; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION; STREPTOCOCCUS PNEUMONIAE;

EID: 27244451533     PISSN: 10168478     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (27)

1. Adam, D. (2002) Global antibiotic resistance in Streptococcus pneumoniae. J. Antimicrob. Chemother. 50, (Topic T1), 1-5.
2. Akerley, B. J., Rubin, E. J., Novick, V. L., Amaya, K., Judson, N., et al. (2002) A genome-scale analysis for identification of genes required for growth or survival of Haemophilus influenzae. Proc. Natl. Acad. Sci. USA 99, 966-971.
3. Apfel, C. M., Locher, H., Evers, S., Takács, B., Hubschwerlen, C., et al. (2001) Peptide deformylase as an antibacterial drug target: target validation and resistance development. Antimicrob. Agents Chemother. 45, 1058-1064.
4. Arigoni, F., Talabot, F., Peitsch, M., Degerton, M. D., Meldrum, E., et al. (1998) A genome-based approach for the identification of essential bacterial genes. Nat. Biotechnol. 16, 851-858.
5. Bruccoleri, R. E., Dougherty, T. J., and Davison, D. B. (1998) Concordance analysis of microbial genomes. Nucleic Acids Res. 16, 4482-4486.
6. Chalker, A. F., Minehart, H. W., Hughes, N. J., Koretke, K. K., Lonetto, M. A., et al. (2001) Systematic identification of selective essential genes in Helicobacter pylori by genome prioritization and allelic replacement mutagenesis. J. Bacteriol. 183, 1259-1268.
7. fcsK) suitable for gene essentiality and antibacterial mode-of-action studies in Streptococcus pneumoniae. J. Bacteriol. 185, 2051-2058.
8. El Zoeiby, A., Sanschagrin, F., and Levesque, R. C. (2003) Structure and function of the Mur enzymes: development of novel inhibitors. Mol. Microbiol. 47, 1-12.
9. Forsyth, R. A., Haselbeck, R. J., Ohlsen, K. L., Yamamoto, R. T., Xu, H., et al. (2002) A genome-wide strategy for the identification of essential genes in Staphylococcus aureus. Mol. Microbiol. 43, 1387-1400.
10. Havarstein, L., Coomaraswamy, G., and Morrison, D. A. (1995) An unmodified heptadecaptide pheromone induces competence for genetic transformation in Streptococcus pneumoniae. Proc. Natl. Acad. Sci. USA 92, 11140-11144.
11. Hoskins, J., Alborn, W. E. Jr, Arnold, J., Blaszczak, L. C., Burgett, S., et al. (2001) Genome of the bacterium Streptococcus pneumoniae strain R6. J. Bacteriol. 183, 5709-5717.
12. Hutchison, C. A., Pterson, S. N., Gill, S. R., Cline, R. T., White, O., et al. (1999) Global transposon mutagenesis and a minimal Mycoplasma genome. Science 286, 2165-2169.
13. Ji, Y., Zhang, B., Van Horn, S. F., Warren, P., Woodnutt, G., et al. (2001) Identification of critical staphylococcal genes using conditional phenotypes generated by antisense RNA. Science 293, 2266-2269.
14. Jordan, I. K., Rogozin, I. B., Wolf, Y. I., and Koonin, E. V. (2002) Essential genes are more evolutionary conserved than are nonessential genes in bacteria. Genome Res. 12, 962-968.
15. Kim, C.-H. (2003) A Salmonella typhimurium rfaE mutant recovers invasiveness for human epithelial cells when complemented by wild type rfaE (controlling, biosynthesis of ADP-L-glycero-D-manno-heptose-containing lipopolysaccharide). Mol. Cells 15, 226-232.
16. Kobayashi, K., Ehrlich, S. D., Albertini, A., Amati, G., Andersen, K. K., et al. (2003) Essential Bacillus subtilis genes. Proc. Natl. Acad. Sci. USA 100, 4678-4683.
17. Mills, S. D. (2003) The role of genomics in antimicrobial discovery. J. Antimicrob. Chemother. 51, 749-752.
18. Paton, J. C., Berry, A. M., Lock, R. A., Hansman, D., and Manning, P. A. (1986) Cloning and expression in Escherichia coli of the Streptococcus pneumoniae gene encoding pneumolysin. Infect. Immun. 54, 50-55.
19. Pierce, B. J., Ianelli, F., and Pozzi, F. (2002) Construction of new unencapsulated (rough) strains of Streptococcus pneumoniae. Res. Microbiol. 153, 243-247.
20. Sassetti, C. M., Boyd, D. H., and Rubin, E. J. (2001) Comprehensive identification of conditionally essential genes in mycobacteria. Proc. Natl. Acad. Sci. USA 98, 12712-12717.
21. Tatusov, R. L., Koonin, E. V., and Lipman, D. J. (1997) A genomic perspective on protein families. Science 278, 631-637.
22. Tettelin, H., Nelson, K. E., Paulsen, I. T., Eisen, J. A., Read, T. D., et al. (2001) Complete genome sequence of a virulent isolate of Streptococcus pneumoniae. Science 293, 498-506.
23. Thanassi, J. A., Hartman-Neumann, S. L., Dougherty, T. J., Dougherty, B. A., and Pucci, M. J. (2002) Identification of 113 conserved essential genes using a high-throughput gene disruption system in Streptococcus pneumoniae. Nucleic Acids Res. 30, 3152-3162.
24. Trieu-Cuot, P. and Courvalin, P. (1983) Nucleotide sequence of the Streptococcus faecalis plasmid gene encoding the 3′5′-aminoglycoside phosphotransferase type III. Gene 23, 331-341.
25. Waller, A. S. and Clements, J. M. (2002) Novel approaches to antimicrobial therapy: peptide deformylase. Curr. Opin. Drug Discov. Devel. 5, 785-792.
26. Zalacain, M., Biswas, S., Ingraham, K. A., Ambrad, J., Bryant, A., et al. (2004) A global approach to identify novel broad-spectrum antibacterial targets among proteins of unknown function. J. Mol. Microbiol. Biotechnol. 6, 109-126.
27. Zhang, R., Ou, Z. Y., and Zhang, C. T. (2004) DEG: a database of essential genes. Nucleic Acids Res. 32, D271-D272.