Triton X-100 pretreatment of LR-white thin sections improves immunofluorescence specificity and intensity

Microscopy Research and Technique

Volumn 70, Issue 7, 2007, Pages 555-562

  Ghrebi, Salem S ^a   Owen, Gethin Rh ^a   Brunette, Donald M ^a  

^a UNIVERSITY OF BRITISH COLUMBIA   (Canada)

Author keywords

Immunostaining; LR White; Macrophage; Postembedding; Triton X 100

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; CELL CULTURE; FLUORESCENCE; RESINS;

ANTIGENICITY; ANTIGENICS; IMMUNOSTAINING; LR-WHITE; PARTIAL EMBEDDING; POSTEMBEDDING; PRE-TREATMENTS; PROCESSING STEPS; THIN-SECTIONS; TRITON X-100;

MACROPHAGES;

ACRYLIC ACID RESIN; ANTIGEN; FIXATIVE; FLUORESCENT DYE; LIPID; RESIN; TRITON X 100;

ANIMAL CELL; ANTIGENICITY; ARTICLE; CELL CULTURE; CELL MEMBRANE PERMEABILITY; CROSS LINKING; EMBEDDING; EXTRACTION; HYDROPHILICITY; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; LABELING INDEX; LYSOSOME; MACROPHAGE; NONHUMAN; PRIORITY JOURNAL; PROTEIN CONFORMATION; SCANNING ELECTRON MICROSCOPY; TECHNIQUE;

EID: 34447267448     PISSN: 1059910X     EISSN: 10970029     Source Type: Journal    
DOI: 10.1002/jemt.20422     Document Type: Article

References (38)

1. Anderson JM. 1993. Mechanisms of inflammation and infection with implanted devices. Cardiovasc Pathol 2:S33-S41.
2. Anderson JM. 2000. The cellular cascades of wound healing. In: Davies JE, editor. Bone engineering. Toronto: Em squared. pp. 81-94.
3. Barsky SH, Rao NC, Restrepo C, Liotta LA. 1984. Immunocytochemical enhancement of basement membrane antigens by pepsin: Applications in diagnostic pathology. Am J Clin Pathol 82:191-194.
4. Beelen RHJ, Eestermans IL, Dopp EA, Dijkstra CD. 1987. Monoclonal antibodies ED1, ED2, and ED3 against rat macrophages: Expression of recognized antigens in different stages of differentiation. Transplant Proc 19:3166-3170.
5. Behrman EJ. 1984. The chemistry of osmium tetroxide fixation. In: Scanning electron microscopy. AMF O'Hare, Chicago, IL: SEM. p.1.
6. Berber JE, Rothe A, Buller G, Bradford J, Gray DR, Filanoski BJ, Telford WG, Yue S, Liu J, Cheung CY, Chang W, Hirsch JD, Beechem JM, Haugland RP, Haugland RP. 2003. Quantitative comparison of long-wavelength Alexa Fluor dyes to Cy dyes: Fluorescence of the dyes and their bioconjugates. J Histochem Cytochem 51:1699-1712.
7. Campoccia D, Hunt AJ, Doherty PJ, Zhong M, O'Regan Benedetti L, Williams DF. 1996. Quantitative assessment of the tissue response to hyaluronan derivatives. Biomaterials 17:963-975.
8. Craig S, Goodchild DJ. 1982. Post-embedding immunolabelling. Some effects of tissue preparation on the antigenicity of plant proteins. Eur J Cell Biol 28:251-256.
9. Damoiseaux JGMC, Dopp EA, Calame W, Chao D, MacPherson GG. 1994. Rat macrophage lysosomal membrane antigen recognized by monoclonal antibody ED1. Immunology 83:140-147.
10. Dijkstra CD, Dopp EA, Joling P, Kraal G. 1985. The heterogeneity of mononuclear phagocytes in lymphoid organs: Distinct macrophage subpopulations in the rat by monoclonal antibodies ED1, ED2, and ED3. Immunology 54:589-599.
11. Goldenthal KL, Hedman K, Chen JW, August JT, Willingham MC. 1985. Postfixation detergent treatment for immunofluorescence suppresses localization of some integral membrane proteins. J Histochem Cytochem 88:813-820.
12. Grube D. 1980. Immunoreactivities of gastrin (G) cells. II. Nonspecific bonding of immunogolobulins to G-cells by ionic interactions. Histochemistry 66:149-167.
13. Helenius A, Simons K. 1975. Solubilization of membranes by detergents. Biochem Biophys Acta 415:29-79.
14. Herken R, Fussek M, Barth S, Gotz W. 1988. LR-White and LR-Gold resins for postembedding immunofluorescence staining of laminin in mouse kidney. Histochem J 20:427-432.
15. Hobot JA, Newman GR. 1991. Strategies for improving the cytochemical and immunocytochemical sensitivity of ultrastructurally well-preserved, resin embedded biological tissue for light and electron microscopy. Scanning Microsc Suppl 5:S27-S41.
16. Hunt JA, Vince DG, Williams DF. 1993. Image analysis in the evaluation of biomaterials. J Biomed Eng 15:39-45.
17. Jones D. 1969. The reaction of formaldehyde with unsaturated fatty acids during histological fixation. Histochem J 1:459-491.
18. Jones MN. 1999. Surfactants in membrane solubilization. Int J Pharm 177:137-159.
19. Kellenberger E, Durrenberger M, Villiger W, Carlemalm E, Wurtz M. 1987. The efficiency of immunolabel on Lowicryl sections compared to theoretical predictions. J Histochem Cytochem 35:959-969.
20. Kraehenbuhl JP, Racine L, Jamieson JD. 1977. Immunocytochemical localization of secretory proteins in bovine exocrine cells. J Cell Biol 72:406-423.
21. Larsson LI. 1981. Peptide immunocytochemistry. Prog Histochem Cytochem 13:1-85.
22. Maldonado IRSC, Ferreira ML, Camargos ERS, Chiari E, Machado CRS. 2004. Skeletal muscle regeneration and Tryponosoma cruzi-induced myositis in rats. Histol Histopathol 19:85-93.
23. McLennan I. 1993. Resident macrophages (ED2- and ED3-positive) do not phagocytose degenerating rat skeletal muscle fibers. Cell Tissue Res 272:193-196.
24. Melan MA, Sluder G. 1992. Redistribution and differential extraction of soluble proteins in permeabilized cultured cells. J Cell Sci 101:731-743.
25. Mohanty M, Hunt JA, Doherty PJ, Annis D, Williams J. 1992. Evaluation of soft tissue response to a poly(urethane urea). Biomaterials 13:651-656.
26. Newman GR. 1987. Use and abuse of LR white. Histochem J 19:118-120.
27. Newman GR, Hobot JA. 1987. Modern Acrylics for post-embedding immunostaining techniques. J Histochem Cytochem 35:971-981.
28. Newman GR, Hobot JA. 1989. Role of tissue processing in colloidal gold methods. In: Hayat MA, editor. Colloidal gold: Principles, methods, and applications, Vol. 2. San Diego, CA: Academic Press. pp. 35-45.
29. Newman GR, Jasani B. 1998. Silver development in microscopy and bioanalysis: A new versatile formulation for modern needs. Histochem J 30:635-645.
30. Newman GR, Jasani B, Williams ED. 1983a. A simple post-embedding system for the rapid demonstration of tissue antigens under the electron microscope. Histochem J 15:543-555.
31. Newman GR, Jasani B, Williams ED. 1983b. The visualization of trace amounts of diaminobenzidine (DAB) polymer by gold-sulphide-silver method. J Microsc (Oxford) 132:RP1-RP2.
32. Osborn M, Weber K. 1982. Immunofluorescence and immunocytochemical procedure with affinity purified antibodies: Tubulin-containing structures. Methods Cell Biol 24:97-132.
33. Prete PSC, Malheiros SVP, Meirelles NC, de Paula E. 2002. Quantitative assessment of human erythrocyte membrane solubilization by Triton X-100. Biophys Chem 97:1-5.
34. Richards RG, Stifanic M, Owen GR, Riehle M, ap Gwynn I, Curtis AS. 2001. Immunogold labelling of fibroblast focal adhesion sites visualized in fixed material using scanning electron microscopy, and living, using internal reflection microscopy. Cell Biol Int 25:1237-1249.
35. Rosengren A, Danielsen N, Burjsten LM. 1997. Inflammatory reaction dependence on implant localization in rat soft tissue models. Biomaterials 18:979-987.
36. Rosengren A, Bjursten LM, Danielsen N, Persson H, Kober M. 1999. Tissue reactions to polyethylene implants with different surface topography. J Mater Sci Mater Med 10:75-82.
37. Sabatini DD, Bensch K, Barnett R. 1963. Cytochemistry and electron microscopy. The preservation of cellular structure and enzymatic activity by aldehyde fixation. J Cell Biol 17:19-58.
38. Yamate J, Maeda M, Benn SJ, Laithwaite JE, Allan A, Ide M, Kuwamura M, Kotani T, Sakuma S, LaMarre J. 2001. Effect of lipopolysaccharide on a macrophage like cell line (HS-P) from a rat histiocytic sarcoma. J Comp Pathol 125:15-24.