Ultrastructural localization of S100A3, a cysteine-rich, calcium binding protein, in human scalp hair shafts revealed by rapid-freezing immunocytochemistry

Journal of Histochemistry and Cytochemistry

Volumn 47, Issue 4, 1999, Pages 525-532

  Takizawa, Takami ^a,b   Takizawa, Toshihiro ^a   Arai, Seiichi ^b   Kizawa, Kenji ^b   Uchiwa, Hideyo ^b   Sasaki, Ichiro ^b   Inoue, Takafumi ^b  

^a JICHI MEDICAL UNIVERSITY   (Japan)

^b KANEBO LTD   (Japan)

Author keywords

Cortex; Cuticle; Cysteine rich calcium binding proteins; Electron microscopy; Hair keratins; Human scalp hair shafts; Immunocytochemistry; Rapid freezing; S100 proteins; S100A3

Indexed keywords

CALCIUM BINDING PROTEIN; CYSTEINE; KERATIN; PROTEIN S 100;

ARTICLE; CELL ULTRASTRUCTURE; CONTROLLED STUDY; FREEZE DRYING; HAIR ANALYSIS; HUMAN; HUMAN TISSUE; IMMUNOCYTOCHEMISTRY; IMMUNOELECTRON MICROSCOPY; IMMUNOFLUORESCENCE MICROSCOPY; PRIORITY JOURNAL; PROTEIN LOCALIZATION; SCALP;

EID: 0032905872     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549904700411     Document Type: Article

References (24)

1. Baden HP, McGilvray N, Lee LD, Baden L, Kubilus J (1980) Comparison of stratum corneum and hair fibrous proteins. J Invest Dermatol 75:311-315
2. 2+ binding S100 proteins in normal human skin and melanocytic lesions. Br J Dermatol 137:39-43
3. Cochran AJ, Lu HF, Li PX, Saxton R, Wen DR (1993) S-100 protein remains a practical marker for melanocytic and other tumours. Melanoma Res 3:325-330
4. Engelkamp D, Schäfer BW, Mattei MG, Erne P, Heizmann CW (1993) Six S100 genes are clustered on human chromosome 1q21: identification of two genes coding for the two previously unreported calcium-binding proteins S100D and S100E. Proc Natl Acad Sci USA 90:6547-6551
5. Föhr UG, Heizmann CW, Engelkamp D, Schäfer BW, Cox JA (1995) Purification and cation binding properties of the recombinant human S100 calcium-binding protein A3, an EF-hand motif protein with high affinity for zinc. J Biol Chem 270:21056-21061
6. Hoffmann HJ, Olsen E, Etzerodt M, Madsen P, Thogersen HC, Kruse T, Celis JE (1994) Psoriasin binds calcium and is upregulated by calcium to levels that resemble those observed in normal skin. J Invest Dermatol 103:370-375
7. Kizawa K, Tsuchimoto S, Hashimoto K, Uchiwa H (1998) Gene expression of mouse S100A3, a cysteine-rich calcium-binding protein, in developing hair follicle. J Invest Dermatol 111:879-886
8. Kizawa K, Uchiwa H, Murakami U (1996) Highly-expressed S100A3, a calcium-binding protein, in human hair cuticle. Biochim Biophys Acta 1312:94-98
9. Lee SC, Kim IG, Marekov LN, O'Keefe EJ, Parry DA, Steinert PM (1993) The structure of human trichohyalin. Potential multiple roles as a functional EF-hand-like calcium-binding protein, a cornified cell envelope precursor, and an intermediate filament-associated (cross-linking) protein. J Biol Chem 268:12164-12176
10. Madsen P, Rasmussen HH, Leffers H, Honore B, Celis JE (1992) Molecular cloning and expression of a novel keratinocyte protein (psoriasis-associated fatty acid-binding protein [PA-FABP]) that is highly up-regulated in psoriatic skin and that shares similarity to fatty acid-binding proteins. J Invest Dermatol 99:299-305
11. Markova NG, Marekov LN, Chipev CC, Gan SQ, Idler WW, Steinen PM (1993) Profilaggrin is a major epidermal calcium-binding protein. Mol Cell Biol 13:613-625
12. Powell BC, Rogers GE (1994) Differentiation in hard keratin tissues: hair and related structures. In Leigh IM, Lane EB, Watt FM, eds. The Keratinocyte Handbook. Cambridge, Cambridge University Press, 401-436
13. Schäfer BW, Heizmann CW (1996) The S100 family of EF-hand calcium-binding proteins: functions and pathology. Trends Biochem Sci 21:134-140
14. 2+-binding S100 proteins in epithelial tumours of the skin. Virchows Arch 432:53-59
15. Swift JA (1968) The electron histochemistry of cystine-containing proteins in thin transverse sections of human hair. J R Microsc Soc 88:449-460
16. Swift JA, Bews B (1974) The chemistry of human hair cuticle. I: A new method for the physical isolation of cuticle. J Soc Cosmet Chem 25:13-22
17. Swift JA, Bews B (1976) The chemistry of human hair cuticle. III: The isolation and amino acid analysis of various subfractions of the cuticle obtained by pronase and trypsin digestion. J Soc Cosmet Chem 27:289-300
18. Takizawa Ta, Takizawa To, Arai S, Osumi M, Saito T (1998a) Ultrastructure of human scalp hair shafts as revealed by freeze-substitution fixation. Anat Rec 251:406-413
19. Takizawa Ta, Takizawa To, Uchiwa H, Arai S (1998b) Ultrastructural localization of hair keratins in human scalp hair shafts as revealed by rapid-freezing immunocytochemistry. Acta Histochem Cytochem 31:237-242
20. Takizawa Ta, Takizawa To, Kizawa K, Uchiwa H, Arai S, Saito T (1996) S100A3, a cysteine-rich calcium-binding protein, is highly expressed in the human hair shaft. Mol Biol Cell 7 (suppl):139a
21. Uchiwa H, Hirano M, Ono Y, Murakami U, Ohta M, Ebina T (1994) Production of anti-hair keratin antibody and its biological activity (1st report): a utilization of rabbit antibody. Jpn J Dermatol 104:855-860 (in Japanese with English abstract)
22. Weterman MA, Stoopen GM, van Muijen GN, Kuznicki J, Ruiter DJ, Bloemers HP (1992) Expression of calcyclin in human melanoma cell lines correlates with metastatic behavior in nude mice. Cancer Res 52:1291-1296
23. Wood L, Carter D, Mills M, Hatzenbuhler N, Vogeli G (1991) Expression of calcyclin, a calcium-binding protein, in the keratogenous region of growing hair follicles. J Invest Dermatol 96:383-387
24. Yokota S, Okada Y (1997) Quantitative evaluation of preparation procedures affecting immunogold staining in post embedding immunocytochemistry. Acta Histochem Cytochem 30:497-504