A novel approach for the construction of plant amiRNA expression vectors

Journal of Biotechnology

Volumn 151, Issue 1, 2011, Pages 9-14

  Yan, Hong ^a,b   Deng, Xuan ^b   Cao, Yinglong ^c   Huang, Junyan ^c   Ma, Lixin ^b   Zhao, Bin ^a  

^a HUAZHONG AGRICULTURAL UNIVERSITY   (China)

^b HUBEI UNIVERSITY   (China)

^c INSTITUTE OF PLANT PROTECTION   (China)

Author keywords

Artificial microRNA; Homologous recombination; Mating assisted genetically integrated cloning; Seamless enzyme free cloning

Indexed keywords

ARABIDOPSIS THALIANA; EXPRESSION CASSETTE; EXPRESSION VECTORS; GENE FUNCTION; HOMOLOGOUS RECOMBINATION; IN-VIVO; MATING-ASSISTED GENETICALLY INTEGRATED CLONING; MICRORNAS; PCR PRODUCTS; SEAMLESS ENZYME-FREE CLONING; TARGET GENES; THALIANA;

CLONING; ENZYMES; ESCHERICHIA COLI; OLIGONUCLEOTIDES; PLANTS (BOTANY); VECTORS;

GENE EXPRESSION;

ARTIFICIAL MICRORNA; MICRORNA; PLANT RNA; PLASMID VECTOR; UNCLASSIFIED DRUG;

ARABIDOPSIS; ARTICLE; CONTROLLED STUDY; ESCHERICHIA COLI; EXPRESSION VECTOR; GENE CASSETTE; GENE SILENCING; HOMOLOGOUS RECOMBINATION; MATING ASSISTED GENETICALLY INTEGRATED CLONING; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SEAMLESS ENZYME FREE CLONING; WILD TYPE;

ARABIDOPSIS; CLONING, MOLECULAR; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, PLANT; GENE SILENCING; GENES, PLANT; GENETIC VECTORS; MICRORNAS; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC;

ARABIDOPSIS THALIANA; ESCHERICHIA COLI;

EID: 78650677081     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2010.10.078     Document Type: Article

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