메뉴 건너뛰기




Volumn 284, Issue 5423, 1999, Pages 2148-2152

EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis

Author keywords

[No Author keywords available]

Indexed keywords

ETHYLENE; OXYGEN RADICAL; PROTEIN EIN2; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

EID: 0033603516     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.284.5423.2148     Document Type: Article
Times cited : (1049)

References (48)
  • 6
    • 0031587872 scopus 로고    scopus 로고
    • Q. Chao et al., Cell 89, 1133 (1997).
    • (1997) Cell , vol.89 , pp. 1133
    • Chao, Q.1
  • 9
  • 13
  • 18
    • 0004904165 scopus 로고    scopus 로고
    • S. A. Oh et al., Plant J. 12, 527 (1997).
    • (1997) Plant J. , vol.12 , pp. 527
    • Oh, S.A.1
  • 19
    • 0000157704 scopus 로고
    • For the chromosome walk, YAC clones were identified from the yUP [J. R. Ecker, Methods 1, 186 (1990)], EG [E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)], and EW [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561(1990)] libraries by colony hybridization (38). The ends of the identified clones were isolated by plasmid rescue (39), by vectorette polymerase chain reaction (PCR) (38), or by inverse PCR (39). PCR products were hybridized to an Arabidopsis λDASH II cDNA library (8). Standard protocols were used for Southern (DNA) blotting, phage lifts, and restriction digestions (40). Genomic clones were radiolabeled and hybridized to Southern blots containing alleles of ein2. Arabidopsis genomic DNA was prepared by cesium gradient centrifugation as described [R. E. Pruitt and E. M. Meyerowitz, J. Mol. Biol. 187, 169 (1986)].
    • (1990) Methods , vol.1 , pp. 186
    • Ecker, J.R.1
  • 20
    • 0025765676 scopus 로고
    • For the chromosome walk, YAC clones were identified from the yUP [J. R. Ecker, Methods 1, 186 (1990)], EG [E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)], and EW [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561(1990)] libraries by colony hybridization (38). The ends of the identified clones were isolated by plasmid rescue (39), by vectorette polymerase chain reaction (PCR) (38), or by inverse PCR (39). PCR products were hybridized to an Arabidopsis λDASH II cDNA library (8). Standard protocols were used for Southern (DNA) blotting, phage lifts, and restriction digestions (40). Genomic clones were radiolabeled and hybridized to Southern blots containing alleles of ein2. Arabidopsis genomic DNA was prepared by cesium gradient centrifugation as described [R. E. Pruitt and E. M. Meyerowitz, J. Mol. Biol. 187, 169 (1986)].
    • (1991) Mol. Gen. Genet. , vol.226 , pp. 484
    • Grill, E.1    Somerville, C.2
  • 21
    • 0025413451 scopus 로고
    • For the chromosome walk, YAC clones were identified from the yUP [J. R. Ecker, Methods 1, 186 (1990)], EG [E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)], and EW [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561(1990)] libraries by colony hybridization (38). The ends of the identified clones were isolated by plasmid rescue (39), by vectorette polymerase chain reaction (PCR) (38), or by inverse PCR (39). PCR products were hybridized to an Arabidopsis λDASH II cDNA library (8). Standard protocols were used for Southern (DNA) blotting, phage lifts, and restriction digestions (40). Genomic clones were radiolabeled and hybridized to Southern blots containing alleles of ein2. Arabidopsis genomic DNA was prepared by cesium gradient centrifugation as described [R. E. Pruitt and E. M. Meyerowitz, J. Mol. Biol. 187, 169 (1986)].
    • (1990) Plant Mol. Biol. , vol.14 , pp. 561
    • Ward, E.R.1    Jen, G.C.2
  • 22
    • 0023053043 scopus 로고
    • For the chromosome walk, YAC clones were identified from the yUP [J. R. Ecker, Methods 1, 186 (1990)], EG [E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)], and EW [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561(1990)] libraries by colony hybridization (38). The ends of the identified clones were isolated by plasmid rescue (39), by vectorette polymerase chain reaction (PCR) (38), or by inverse PCR (39). PCR products were hybridized to an Arabidopsis λDASH II cDNA library (8). Standard protocols were used for Southern (DNA) blotting, phage lifts, and restriction digestions (40). Genomic clones were radiolabeled and hybridized to Southern blots containing alleles of ein2. Arabidopsis genomic DNA was prepared by cesium gradient centrifugation as described [R. E. Pruitt and E. M. Meyerowitz, J. Mol. Biol. 187, 169 (1986)].
    • (1986) J. Mol. Biol. , vol.187 , pp. 169
    • Pruitt, R.E.1    Meyerowitz, E.M.2
  • 23
    • 0345428073 scopus 로고    scopus 로고
    • unpublished data
    • Standard methods were used for phage preparation (40). The pgEE1.2 genomic insert was used to isolate clones from a cDNA library (8) and to identify the λgE2 clone from an Arabidopsis λDASH II genomic library (M. Rothenberg and J. R. Ecker, unpublished data).
    • Rothenberg, M.1    Ecker, J.R.2
  • 24
    • 0344134375 scopus 로고    scopus 로고
    • note
    • Arabidopsis genomic DNA was sequenced as described (6).
  • 25
    • 0000047029 scopus 로고
    • Plant transformation was performed as described (6). Regions of the EIN2 cDNA (pcE2.17) were subcloned into pRok2 [D. Baulcombe, G. Saunders, M. Bevan, M. Mayo, B. Harrison, Nature 321, 446 (1986)]. Fragments of EIN2 cDNA that were expressed under the control of the CaMV 355 promoter include the full-length cDNA (amino acids 1 to 1294), terminal deletions of the COOH end (amino acids 1 to 933, 1 to 854, and 1 to 476), an internal deletion (amino acids 89 to 228 removed), and the COOH end (CEND; amino acids 454 to 1294). From 100 ein2:CEND transgenic plants produced, 7 lines that expressed EIN2 at a high level showed constitutive ethylene response phenotypes as adults.
    • (1986) Nature , vol.321 , pp. 446
    • Baulcombe, D.1    Saunders, G.2    Bevan, M.3    Mayo, M.4    Harrison, B.5
  • 28
    • 0031966684 scopus 로고    scopus 로고
    • J. C. Fleet, Nutr. Rev. 56, 88 (1998); F. Supek, L. Supekora, H. Nelson, N. Nelson, J. Exp. Biol. 200, 321 (1997).
    • (1998) Nutr. Rev. , vol.56 , pp. 88
    • Fleet, J.C.1
  • 31
    • 0030755366 scopus 로고    scopus 로고
    • F. Supek, L. Supekova, H. Nelson, N. Nelson, Proc. Natl. Acad. Sci. U.S.A 93, 5105 (1996); M. B. Gunshin et al., Nature 388, 482 (1997); E. Pinner, S. Gruenheid, M. Raymond, P. Gros, J. Biol.Chem. 272, 28933 (1997).
    • (1997) Nature , vol.388 , pp. 482
    • Gunshin, M.B.1
  • 32
    • 0030684012 scopus 로고    scopus 로고
    • F. Supek, L. Supekova, H. Nelson, N. Nelson, Proc. Natl. Acad. Sci. U.S.A 93, 5105 (1996); M. B. Gunshin et al., Nature 388, 482 (1997); E. Pinner, S. Gruenheid, M. Raymond, P. Gros, J. Biol.Chem. 272, 28933 (1997).
    • (1997) J. Biol.chem. , vol.272 , pp. 28933
    • Pinner, E.1    Gruenheid, S.2    Raymond, M.3    Gros, P.4
  • 34
    • 0344134372 scopus 로고    scopus 로고
    • note
    • Screening of T-DNA populations by PCR was done as described (10), with primers for At-Nramp2. The T-DNA insertion site was located 52 nucleotides upstream of the coding region.
  • 37
    • 0033617292 scopus 로고    scopus 로고
    • T. Hirayama et al., Cell 97, 383 (1999).
    • (1999) Cell , vol.97 , pp. 383
    • Hirayama, T.1
  • 44
    • 0003065824 scopus 로고
    • C. Koncz, N.-H. Chua, J. Schell, Eds. World Scientific, Singapore
    • E. Matallana et al., Methods in Arabidopsis Research, C. Koncz, N.-H. Chua, J. Schell, Eds. (World Scientific, Singapore, 1992), p. 144.
    • (1992) Methods in Arabidopsis Research , pp. 144
    • Matallana, E.1
  • 47
    • 0345428069 scopus 로고    scopus 로고
    • note
    • Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
  • 48
    • 0344565941 scopus 로고    scopus 로고
    • note
    • Supported by the Spanish Ministerio de Educacion y Ciencía (J.M.A) and the Human Frontier Science Program Organization (T.H.), and by grants from NSF and the U.S. Department of Energy (J.R.E.).


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.