-
2
-
-
0001403886
-
-
A. B. Bleecker, M. A. Estelle, C. Somerville, H. Kende, Science 241, 1086 (1988).
-
(1988)
Science
, vol.241
, pp. 1086
-
-
Bleecker, A.B.1
Estelle, M.A.2
Somerville, C.3
Kende, H.4
-
4
-
-
0028940855
-
-
G. Roman, B. Lubarsky, J. Kieber, M. Rothenberg, J. Ecker, Genetics 139, 1393 (1995).
-
(1995)
Genetics
, vol.139
, pp. 1393
-
-
Roman, G.1
Lubarsky, B.2
Kieber, J.3
Rothenberg, M.4
Ecker, J.5
-
6
-
-
0031587872
-
-
Q. Chao et al., Cell 89, 1133 (1997).
-
(1997)
Cell
, vol.89
, pp. 1133
-
-
Chao, Q.1
-
7
-
-
0001281970
-
-
D. van der Straeten, A. Djudzman, W. V. Caeneghem, J. Smalle, M. van Montagu. Plant Physiol. 102, 401 (1993).
-
(1993)
Plant Physiol.
, vol.102
, pp. 401
-
-
Van Der Straeten, D.1
Djudzman, A.2
Caeneghem, W.V.3
Smalle, J.4
Van Montagu, M.5
-
8
-
-
0027478967
-
-
J. J. Kieber, M. Rothenberg, G. Roman, K. A. Feldmann, J. R. Ecker, Cell 72, 427 (1993).
-
(1993)
Cell
, vol.72
, pp. 427
-
-
Kieber, J.J.1
Rothenberg, M.2
Roman, G.3
Feldmann, K.A.4
Ecker, J.R.5
-
9
-
-
0028999892
-
-
J. R. Ecker, Science 268, 667 (1995).
-
(1995)
Science
, vol.268
, pp. 667
-
-
Ecker, J.R.1
-
13
-
-
0032417391
-
-
R. Solano et al., Genes Dev. 12, 3703 (1998).
-
(1998)
Genes Dev.
, vol.12
, pp. 3703
-
-
Solano, R.1
-
18
-
-
0004904165
-
-
S. A. Oh et al., Plant J. 12, 527 (1997).
-
(1997)
Plant J.
, vol.12
, pp. 527
-
-
Oh, S.A.1
-
19
-
-
0000157704
-
-
For the chromosome walk, YAC clones were identified from the yUP [J. R. Ecker, Methods 1, 186 (1990)], EG [E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)], and EW [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561(1990)] libraries by colony hybridization (38). The ends of the identified clones were isolated by plasmid rescue (39), by vectorette polymerase chain reaction (PCR) (38), or by inverse PCR (39). PCR products were hybridized to an Arabidopsis λDASH II cDNA library (8). Standard protocols were used for Southern (DNA) blotting, phage lifts, and restriction digestions (40). Genomic clones were radiolabeled and hybridized to Southern blots containing alleles of ein2. Arabidopsis genomic DNA was prepared by cesium gradient centrifugation as described [R. E. Pruitt and E. M. Meyerowitz, J. Mol. Biol. 187, 169 (1986)].
-
(1990)
Methods
, vol.1
, pp. 186
-
-
Ecker, J.R.1
-
20
-
-
0025765676
-
-
For the chromosome walk, YAC clones were identified from the yUP [J. R. Ecker, Methods 1, 186 (1990)], EG [E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)], and EW [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561(1990)] libraries by colony hybridization (38). The ends of the identified clones were isolated by plasmid rescue (39), by vectorette polymerase chain reaction (PCR) (38), or by inverse PCR (39). PCR products were hybridized to an Arabidopsis λDASH II cDNA library (8). Standard protocols were used for Southern (DNA) blotting, phage lifts, and restriction digestions (40). Genomic clones were radiolabeled and hybridized to Southern blots containing alleles of ein2. Arabidopsis genomic DNA was prepared by cesium gradient centrifugation as described [R. E. Pruitt and E. M. Meyerowitz, J. Mol. Biol. 187, 169 (1986)].
-
(1991)
Mol. Gen. Genet.
, vol.226
, pp. 484
-
-
Grill, E.1
Somerville, C.2
-
21
-
-
0025413451
-
-
For the chromosome walk, YAC clones were identified from the yUP [J. R. Ecker, Methods 1, 186 (1990)], EG [E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)], and EW [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561(1990)] libraries by colony hybridization (38). The ends of the identified clones were isolated by plasmid rescue (39), by vectorette polymerase chain reaction (PCR) (38), or by inverse PCR (39). PCR products were hybridized to an Arabidopsis λDASH II cDNA library (8). Standard protocols were used for Southern (DNA) blotting, phage lifts, and restriction digestions (40). Genomic clones were radiolabeled and hybridized to Southern blots containing alleles of ein2. Arabidopsis genomic DNA was prepared by cesium gradient centrifugation as described [R. E. Pruitt and E. M. Meyerowitz, J. Mol. Biol. 187, 169 (1986)].
-
(1990)
Plant Mol. Biol.
, vol.14
, pp. 561
-
-
Ward, E.R.1
Jen, G.C.2
-
22
-
-
0023053043
-
-
For the chromosome walk, YAC clones were identified from the yUP [J. R. Ecker, Methods 1, 186 (1990)], EG [E. Grill and C. Somerville, Mol. Gen. Genet. 226, 484 (1991)], and EW [E. R. Ward and G. C. Jen, Plant Mol. Biol. 14, 561(1990)] libraries by colony hybridization (38). The ends of the identified clones were isolated by plasmid rescue (39), by vectorette polymerase chain reaction (PCR) (38), or by inverse PCR (39). PCR products were hybridized to an Arabidopsis λDASH II cDNA library (8). Standard protocols were used for Southern (DNA) blotting, phage lifts, and restriction digestions (40). Genomic clones were radiolabeled and hybridized to Southern blots containing alleles of ein2. Arabidopsis genomic DNA was prepared by cesium gradient centrifugation as described [R. E. Pruitt and E. M. Meyerowitz, J. Mol. Biol. 187, 169 (1986)].
-
(1986)
J. Mol. Biol.
, vol.187
, pp. 169
-
-
Pruitt, R.E.1
Meyerowitz, E.M.2
-
23
-
-
0345428073
-
-
unpublished data
-
Standard methods were used for phage preparation (40). The pgEE1.2 genomic insert was used to isolate clones from a cDNA library (8) and to identify the λgE2 clone from an Arabidopsis λDASH II genomic library (M. Rothenberg and J. R. Ecker, unpublished data).
-
-
-
Rothenberg, M.1
Ecker, J.R.2
-
24
-
-
0344134375
-
-
note
-
Arabidopsis genomic DNA was sequenced as described (6).
-
-
-
-
25
-
-
0000047029
-
-
Plant transformation was performed as described (6). Regions of the EIN2 cDNA (pcE2.17) were subcloned into pRok2 [D. Baulcombe, G. Saunders, M. Bevan, M. Mayo, B. Harrison, Nature 321, 446 (1986)]. Fragments of EIN2 cDNA that were expressed under the control of the CaMV 355 promoter include the full-length cDNA (amino acids 1 to 1294), terminal deletions of the COOH end (amino acids 1 to 933, 1 to 854, and 1 to 476), an internal deletion (amino acids 89 to 228 removed), and the COOH end (CEND; amino acids 454 to 1294). From 100 ein2:CEND transgenic plants produced, 7 lines that expressed EIN2 at a high level showed constitutive ethylene response phenotypes as adults.
-
(1986)
Nature
, vol.321
, pp. 446
-
-
Baulcombe, D.1
Saunders, G.2
Bevan, M.3
Mayo, M.4
Harrison, B.5
-
28
-
-
0031966684
-
-
J. C. Fleet, Nutr. Rev. 56, 88 (1998); F. Supek, L. Supekora, H. Nelson, N. Nelson, J. Exp. Biol. 200, 321 (1997).
-
(1998)
Nutr. Rev.
, vol.56
, pp. 88
-
-
Fleet, J.C.1
-
29
-
-
0030910763
-
-
J. C. Fleet, Nutr. Rev. 56, 88 (1998); F. Supek, L. Supekora, H. Nelson, N. Nelson, J. Exp. Biol. 200, 321 (1997).
-
(1997)
J. Exp. Biol.
, vol.200
, pp. 321
-
-
Supek, F.1
Supekora, L.2
Nelson, H.3
Nelson, N.4
-
30
-
-
0029978512
-
-
F. Supek, L. Supekova, H. Nelson, N. Nelson, Proc. Natl. Acad. Sci. U.S.A 93, 5105 (1996); M. B. Gunshin et al., Nature 388, 482 (1997); E. Pinner, S. Gruenheid, M. Raymond, P. Gros, J. Biol.Chem. 272, 28933 (1997).
-
(1996)
Proc. Natl. Acad. Sci. U.S.A
, vol.93
, pp. 5105
-
-
Supek, F.1
Supekova, L.2
Nelson, H.3
Nelson, N.4
-
31
-
-
0030755366
-
-
F. Supek, L. Supekova, H. Nelson, N. Nelson, Proc. Natl. Acad. Sci. U.S.A 93, 5105 (1996); M. B. Gunshin et al., Nature 388, 482 (1997); E. Pinner, S. Gruenheid, M. Raymond, P. Gros, J. Biol.Chem. 272, 28933 (1997).
-
(1997)
Nature
, vol.388
, pp. 482
-
-
Gunshin, M.B.1
-
32
-
-
0030684012
-
-
F. Supek, L. Supekova, H. Nelson, N. Nelson, Proc. Natl. Acad. Sci. U.S.A 93, 5105 (1996); M. B. Gunshin et al., Nature 388, 482 (1997); E. Pinner, S. Gruenheid, M. Raymond, P. Gros, J. Biol.Chem. 272, 28933 (1997).
-
(1997)
J. Biol.chem.
, vol.272
, pp. 28933
-
-
Pinner, E.1
Gruenheid, S.2
Raymond, M.3
Gros, P.4
-
33
-
-
0028797538
-
-
V. Rodrigues, P. Y. Cheah, K. Ray, W. Chia, EMBO J. 14, 3007 (1995).
-
(1995)
EMBO J.
, vol.14
, pp. 3007
-
-
Rodrigues, V.1
Cheah, P.Y.2
Ray, K.3
Chia, W.4
-
34
-
-
0344134372
-
-
note
-
Screening of T-DNA populations by PCR was done as described (10), with primers for At-Nramp2. The T-DNA insertion site was located 52 nucleotides upstream of the coding region.
-
-
-
-
37
-
-
0033617292
-
-
T. Hirayama et al., Cell 97, 383 (1999).
-
(1999)
Cell
, vol.97
, pp. 383
-
-
Hirayama, T.1
-
39
-
-
0030898097
-
-
H. M. Smalle, J. Kurepa, M. van Montagu, D. van der Straeten, Proc. Natl. Acad. Sci. U.S.A. 94, 2756 (1997).
-
(1997)
Proc. Natl. Acad. Sci. U.S.A.
, vol.94
, pp. 2756
-
-
Smalle, H.M.1
Kurepa, J.2
Van Montagu, M.3
Van Der Straeten, D.4
-
40
-
-
0032297191
-
-
I. A. Penninckx, B. P. Thomma, A. Buchala, J. P. Metraux, Plant Cell 10, 2103 (1998).
-
(1998)
Plant Cell
, vol.10
, pp. 2103
-
-
Penninckx, I.A.1
Thomma, B.P.2
Buchala, A.3
Metraux, J.P.4
-
41
-
-
0344565939
-
-
data not shown
-
J. M. Alonso, T. Hirayama, G. Roman, S. Nourizadeh, J. R. Ecker, data not shown.
-
-
-
Alonso, J.M.1
Hirayama, T.2
Roman, G.3
Nourizadeh, S.4
Ecker, J.R.5
-
44
-
-
0003065824
-
-
C. Koncz, N.-H. Chua, J. Schell, Eds. World Scientific, Singapore
-
E. Matallana et al., Methods in Arabidopsis Research, C. Koncz, N.-H. Chua, J. Schell, Eds. (World Scientific, Singapore, 1992), p. 144.
-
(1992)
Methods in Arabidopsis Research
, pp. 144
-
-
Matallana, E.1
-
46
-
-
0004136246
-
-
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
-
J. Sambrook, E. Fritsch, T. Maniatis, Molecular Cloning: a Laboratory Manual (Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1989).
-
(1989)
Molecular Cloning: A Laboratory Manual
-
-
Sambrook, J.1
Fritsch, E.2
Maniatis, T.3
-
47
-
-
0345428069
-
-
note
-
Abbreviations for the amino acid residues are as follows: A, Ala; C, Cys; D, Asp; E, Glu; F, Phe; G, Gly; H, His; I, Ile; K, Lys; L, Leu; M, Met; N, Asn; P, Pro; Q, Gln; R, Arg; S, Ser; T, Thr; V, Val; W, Trp; and Y, Tyr.
-
-
-
-
48
-
-
0344565941
-
-
note
-
Supported by the Spanish Ministerio de Educacion y Ciencía (J.M.A) and the Human Frontier Science Program Organization (T.H.), and by grants from NSF and the U.S. Department of Energy (J.R.E.).
-
-
-
|