Construct design for efficient, effective and high-throughput gene silencing in plants

Plant Journal

Volumn 27, Issue 6, 2001, Pages 581-590

  Varsha Wesley, S ^a   Helliwell, Christopher A ^a   Smith, Neil A ^a   Wang, Mingbo ^a   Rouse, Dean T ^a   Liu, Qing ^a   Gooding, Paul S ^a   Singh, Surinder P ^a   Abbott, David ^a   Stoutjesdijk, Peter A ^a   Robinson, Simon P ^a   Gleave, Andrew P ^b   Green, Allan G ^a   Waterhouse, Peter M ^a  

^a CSIRO   (Australia)

^b THE NEW ZEALAND INSTITUTE FOR PLANT AND FOOD RESEARCH LIMITED   (New Zealand)

Author keywords

Gateway; Genomics; ihpRNA; PTGS; RNAi; Vector

Indexed keywords

EXPRESSED SEQUENCE TAG; GENE EXPRESSION SYSTEM; GENE SILENCING; GENETIC TRANSCRIPTION; HAIRPIN RNA; INTRON; MOLECULAR CLONING; PLANT GENETICS; TRANSGENIC PLANT;

CLONING; ENZYMES; GENES; RNA;

GENE SILENCING;

PLANTS (BOTANY);

ARABIDOPSIS; GENE SILENCING; GENETIC VECTORS; INTRONS; MOLECULAR PROBE TECHNIQUES; MOLECULAR SEQUENCE DATA; NUCLEIC ACID CONFORMATION; ORYZA SATIVA; PLANTS, GENETICALLY MODIFIED; RESEARCH DESIGN; RNA PROBES; RNA SPLICING; RNA, ANTISENSE; TOBACCO; TRANSFORMATION, GENETIC;

EID: 0034800385     PISSN: 09607412     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1365-313X.2001.01105.x     Document Type: Article

References (31)

1. EIN2, a bifunctional transducer of ethylene and stress responses in Arabidopsis
2. T-DNA insertion and mutagenesis in Arabidopsis: Going back and forth
3. Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
4. Transformation of an Australian cotton cultivar: Prospects for cotton improvement through genetic engineering
5. RNA interference is mediated by 21- and 22-nucleotide RNAs
6. Maize Adh1 promoter sequences control anaerobic regulation: Addition of upstream promoter elements from constitutive genes is necessary for expression in tobacco
7. Potent and specific genetic interference by double stranded RNA in Caenorhabditis elegans
8. Functional genomic analysis of C. elegans chromosome I by systematic RNA interference
9. Genetic control of the conversion of dihydroflavonols and anthocyanins in flowers of Petunia hybrida
10. A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome
11. Functional genomic analysis of cell division in C. elegans using RNAi of genes on chromosome III
12. A species of small antisense RNA in post-transcriptional gene silencing in plants
13. A transgene with repeated DNA causes high frequency post-transcriptional suppression of ACC-oxidase gene expression in tomato
14. GUS fusion: β-glucuronidase as a sensitive and versatile gene fusion marker in higher plants
15. The isolation and characterization of gibberellin-deficient mutants in tomato
16. T-DNA as an insertional mutagen in Arabidopsis
17. Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana
18. Functional genomics: Probing plant gene function and expression with transposons
19. Broad bean leaf polyphenol oxidase is a 60-kilodalton protein susceptible to proteolytic cleavage
20. Large-scale analysis of the yeast genome by transposon tagging and gene disruption
21. The molecular basis of vernalization: The central role of FLOWERING LOCUS C (FLC)
22. Total silencing by intron-spliced hairpin RNAs
23. High-efficiency silencing of a β-glucuronidase gene in rice is correlated with repetitive transgene structure but independent of DNA methylation
24. Improved vectors for Agrobacterium tumefaciens-mediated transformation of monocot plants
25. Intron-mediated improvement of a selectable marker gene for plant transformation using Agrobacterium tumefaciens
26. Replicating satellite RNA induces sequence-specific DNA methylation and truncated transcripts in plants
27. Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA
28. Gene silencing as an adaptive defence against viruses
29. RNAi: Double-stranded RNA directs the ATP-dependent cleavage of mRNA at 21-23 nucleotide intervals