Laser scanning cytometry for automation of the micronucleus assay

Mutagenesis

Volumn 26, Issue 1, 2011, Pages 153-161

  Darzynkiewicz, Zbigniew ^a   Smolewski, Piotr ^a,b   Holden, Elena ^c   Luther, Ed ^c   Henriksen, Mel ^c   Franois, Maxime ^d,e   Leifert, Wayne ^d   Fenech, Michael ^d  

^a NEW YORK MEDICAL COLLEGE   (United States)

^b MEDICAL UNIVERSITY OF LODZ   (Poland)

^c CompuCyte Corporation ^*  (United States)

^d CSIRO   (Australia)

^e EDITH COWAN UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

CYTOCHALASIN B; MITOMYCIN C;

AUTOMATION; CELL STRAIN HL 60; CELL STRAIN MCF 7; CELL STRAIN U937; CHEEK MUCOSA; CONTROLLED STUDY; CYTOKINESIS; DNA CONTENT; ERYTHROCYTE; FLOW CYTOMETRY; GENOTOXICITY; HUMAN; HUMAN CELL; LASER SCANNING CYTOMETRY; MICRONUCLEUS TEST; MOLECULAR PROBE; MUTAGENICITY; PRIORITY JOURNAL; PROTEIN CONTENT; REVIEW;

ANIMALS; CHO CELLS; CRICETINAE; CRICETULUS; DNA; ERYTHROCYTES; FLOW CYTOMETRY; HUMANS; IMAGE PROCESSING, COMPUTER-ASSISTED; LASER SCANNING CYTOMETRY; MICRONUCLEUS TESTS; MOUTH MUCOSA;

MAMMALIA;

EID: 78650646592     PISSN: 02678357     EISSN: 14643804     Source Type: Journal    
DOI: 10.1093/mutage/geq069     Document Type: Review

References (50)

1. Fenech, M. (2000) The in vitro micronucleus technique. Mutat. Res., 455, 81-95.
2. Fenech, M. (2006) Cytokinesis-block micronucleus assay evolves into a ''cytome'' assay of chromosomal instability, mitotic dysfunction and cell death. Mutat. Res., 600, 58-66.
3. Thomas, P., Holland, N., Bolognesi, C., Kirch-Volders, M., Bonassi, S., Zeiger, E., Knassmueller, S. and Fenech, M. (2009) Buccal micronucleus cytome assay. Nat. Protoc., 4, 825-837.
4. Nüsse, M. (1981) Cell cycle kinetics of irradiated synchronous and asynchronous tumor cells with DNA distribution analysis and BrdUrd-Hoechst 33258 technique. Cytometry, 2, 70-79.
5. Kramer, J., Schaich-Walch, G. and Nüsse, M. (1990) DNA synthesis in radiation induced micronuclei studied by bromodeoxyuridine (BrdUrd) labeling and anti-BrdUrd antibodies. Mutagenesis, 5, 491-495.
6. Fenech, M., Bonassi, S., Turner, J. et al. (2003) HUman MicroNucleus project. Intra- and inter-laboratory variation in the scoring of micronuclei and nucleoplasmic bridges in binucleated human lymphocytes. Results of an international slide-scoring exercise by the HUMN project. Mutat. Res., 534, 45-64. (Pubitemid 36015838)
7. Fenech, M., Jarvis, L. R. and Morley, A. A. (1988) Preliminary studies on scoring micronuclei by computerized image analysis. Mutat. Res., 203, 33-38.
8. Hayashi, M., Norppa, H., Sofuni, T. and Ishidate, M. (1990) Automation of mouse micronucleus test by flow cytometry and image analysis. Cytometry Suppl., 4, 35.
9. Tates, A. D., van Wellie, M. T. and Ploem, J. S. (1990) The present state of the automated micronucleus test for lymphocytes. Int. J. Radiat. Biol., 58, 813-825.
10. Verhaegen, F., Vral, A., Seuntjens, J., Schipper, N. M., de Ridder, N. W. and Thierens, H. (1994) Scoring of radiation-induced micronuclei in cytokinesis-blocked human lymphocytes by automated image analysis. Cytometry, 17, 119-127.
11. Bocker, W., Streffer, C., Muller, W. U. and Yu, C. (1996) Automated scoring of micronuclei in binucleated human lymphocytes. Int. J. Radiat. Biol., 70, 529-537.
12. Thierens, H., Vral, A., De Scheerder, F., De Ridder, L. and Tates, A. (1997) Semi-automated micronucleus scoring in cytokinesis-blocked lymphocytes after irradiation. Int. J. Radiat. Biol., 72, 319-324.
13. Frieauff, W., Potter-Locher, F., Cordier, A. and Suter, W. (1998) Automatic analysis of the in vitro micronucleus test on V79 cells. Mutat. Res., 413, 57-68.
14. Castelain, P., VanHummelen, P., Deleener, A. and Kirsch-Volders, M. (1993) Automated detection of cytocholasin-B blocked binucleated lymphocytes for scoring micronuclei. Mutagenesis, 8, 285-293.
15. Decordier, I., Papine, A., Plas, G. et al. (2009) Automated image analysis of cytokinesis-blocked micronuclei: an adapted protocol and a validated scoring procedure for biomonitoring. Mutagenesis, 24, 85-93.
16. Ludwikow, G., Stalnacke, C. G., Johanson, K. J., Sundell-Bergman, S. and Richter, S. (1990) Microscopic and flow cytometric study of micronuclei in iododeoxyuridine labelled cells irradiated with soft X-rays. Acta Oncol., 29, 761-767.
17. Tometsko, A. M. and Leary, J. F. (1990) A peripheral blood micronucleus assay based on flow cytometry. Cytometry Suppl., 4, 35.
18. Hayashi, M., Norrpa, H., Sofuni, T. and Ishidate, M., Jr. (1992) Mouse bone marrow micronucleus test using flow cytometry. Mutagenesis, 7, 251-256.
19. Schreiber, G. A., Beisker, W., Bauchinger, M. and Nüsse, M. (1992) Multiparametric flow cytometric analysis of radiation-induced micronuclei in mammalian cell cultures. Cytometry, 13, 90-102.
20. Nusse, M., Beisker, W., Kramer, J., Miller, B. M., Schreiber, G. A., Viaggi, S., Weller, E. M. and Wessels, J. M. (1994) Measurement of micronuclei by flow cytometry. Methods Cell Biol, 42, 149-160.
21. Nusse, M. and Marx, K. (1997) Flow cytometric analysis of micronuclei in cell cultures and human lymphocytes: advantages and disadvantages. Mutat. Res., 392, 109-115.
22. Fiedler, R. D., Weiner, S. K. and Schuler, M. (2010) Evaluation of a modified CD71 MicroFlow® method for the flow cytometric analysis of micronuclei in rat bone marrow erythrocytes. Mutat. Res., 2010 Aug. 17 [Epub ahead of print].
23. Cammerer, Z., Schumacher, M. M., Kirsch-Volders, M., Suter, W. and Elhajouji, A. (2010) Flow cytometry peripheral blood micronucleus test in vivo: determination of potential thresholds for aneuploidy induced by spindle poisons. Environ. Mol. Mutagen. 51, 278-284.
24. Liu, L., Liu, Y., Ni, G. and Liu, S. (2010) Flow cytometric scoring of micronucleated reticulocytes as a possible high-throughput radiation biodosimeter. Environ. Mol. Mutagen., 51, 215-221.
25. Elhajoui, A., Van Hummelen, P. and Kirsch-Volders, M. (1995) Indications for a threshold of chemically-induced aneuploidy in vitro in human lymphocytes. Environ. Mol. Mutagen., 26, 292-304.
26. Avlasevich, S. L., Bryce, S. M., De Boeck, M., Elhajouji, A., Van Goethem, F., Lynch, A., Nicolette, J., Shi, J. and Dertinger, S. (2010) Flow cytometric analysis of in vitro mammalian cell micronuclei: past, present, and future. Mutagenesis, 26, 147-152.
27. Avlasevich, S. L., Bryce, S. M., Cairns, S. E. and Dertinger, S. D. (2006) In vitro micronucleus scoring by flow cytometry: differential staining of micronuclei versus apoptotic and necrotic chromatin enhances assay reliability. Environ. Mol. Mutagen., 47, 56-66.
28. Halicka, H. D., Bedner, E. and Darzynkiewicz, Z. (2000) Segregation of RNA and separate packaging of DNA and RNA in apoptotic bodies during apoptosis. Exp. Cell Res., 260, 248-255.
29. Fenech, M. (2007) Cytokinesis-block micronucleus cytome assay. Nat. Protoc, 2, 1084-1094.
30. Kamentsky, L. A. (2001) Laser scanning cytometry. Methods Cell Biol., 63, 51-87.
31. Darzynkiewicz, Z., Bedner, E., Li, X., Gorczyca, W. and Melamed, M. R. (1999) Laser scanning cytometry. A new instrumentation with many applications. Exp. Cell. Res., 249, 1-12.
32. Henriksen, M. (2010) Quantitative imaging cytometry: instrumentation of choice for automated cellular and tissue analysis. Nat. Methods, 7, 331-332.
33. Luther, E., Kamentsky, L., Henriksen, M. and Holden, E. (2004) Next-generation laser scanning cytometry. Methods Cell Biol., 75, 185-218.
34. Peterson, R. A., Krull, D. L. and Butler, L. (2008) Applications of laser scanning cytometry in immunohistochemistry and routine histopathology. Toxicol. Pathol., 36, 117-132.
35. Pozarowski, P., Holden, E. and Darzynkiewicz, Z. (2005) Laser scanning cytometry. Principles and applications. Methods Mol. Biol., 319, 165-192.
36. Styles, J. A., Clark, H., Festing, M. F. and Rew, D. A. (2001) Automation of mouse micronucleus genotoxicity assay by laser scanning cytometry. Cytometry, 44, 153-155.
37. Huang, X., Okafuji, M., Traganos, F., Luther, E., Holden, E. and Darzynkiewicz, Z. (2004) Assessment of histone H2AX phosphorylation induced by DNA topoisomerase I and II inhibitors topotecan and mitoxantrone and by DNA crosslinking agent cisplatin. Cytometry A, 58A, 99-110.
38. Smolewski, P., Ruan, Q., Vellon, L. and Darzynkiewicz, Z. (2001) The micronuclei assay by laser scanning cytometry. Cytometry, 45, 19-26. (Pubitemid 32807920)
39. Zhao, H., Traganos, F. and Darzynkiewicz, Z. (2010) Kinetics of the UV-induced DNA damage response in relation to cell cycle phase. Correlation with DNA replication. Cytometry A, 77A, 285-293.
40. Tanaka, T., Huang, X., Halicka, H. D., Zhao, H., Traganos, F., Albino, A. P., Dai, W. and Darzynkiewicz, Z. (2007) Cytometry of ATM activation and histone H2AX phosphorylation to estimate extent of DNA damage induced by exogenous agents. Cytometry A, 71A, 648-661.
41. Crissman, H. A., Darzynkiewicz, Z., Tobey, R. A. and Steinkamp, J. A. (1985) Correlated measurements of DNA, RNA and protein content in individual cells by flow cytometry. Science, 228, 1321-1324. (Pubitemid 15000358)
42. Darzynkiewicz, Z. (2010) Critical aspects in analysis of cellular DNA content. Curr. Protoc. Cytom., Chapter 7 Unit 7.2, pp. 7.2.1-7.2.8.
43. Kamentsky, L. A., Kamentsky, L. D., Fletcher, J. A., Kurose, A. and Sasaki, K. (1997) Methods for automatic multiparameter analysis of fluorescence in situ hybridized specimens with a laser scanning cytometer. Cytometry, 27, 117-125.
44. Bedner, E., Ruan, Q., Chen, S., Kamentsky, L. A. and Darzynkiewicz, Z. (2000) Multiparameter analysis of progeny of individual cells in clonogenicity assays by laser scanning cytometry (LSC). Cytometry, 40, 271-279.
45. Thomas, P., Harvey, S., Gruner, T. and Fenech, M. (2008) The buccal cytome and micronucleus frequency is substantially altered in Down's syndrome and normal ageing compared to young healthy controls. Mutat. Res., 638, 37-47.
46. Heddle, J., Fenech, M., Hayashi, M. and MacGregor, J. T. (2010) Reflections of the development of micronucleus assays. Mutagenesis, 26, 3-10.
47. Hutter, K. J. and Stöhr, M. (1982) Rapid detection of mutagen induced micronucleated erythrocytes by flow cytometry. Histochemistry, 75, 353-362.
48. Grawe, J. (2005) Flow cytometric analysis of micronuclei in erythrocytes. Methods Mol. Biol., 291, 69-83.
49. Criswell, K. A., Krishna, G., Zielinski, D., Urda, G. A., Theiss, J. C., Juneau, P. and Bleavins, M. R. (1998) Use of acridine orange in flow cytometric assessment of micronuclei induction. Mutat. Res., 414, 63-75. (Pubitemid 28284941)
50. Grawe, J., Zetterberg, G. and Amneus, H. (1993) DNA content determination of micronucleated polychromatic erythrocytes induced by clastogens and spindle poisons in mouse bone marrow and peripheral blood. Mutagenesis, 8, 249-255.