MALDI-TOF-mass spectrometry applications in clinical microbiology

Future Microbiology

Volumn 5, Issue 11, 2010, Pages 1733-1754

  Seng, Piseth ^a   Rolain, Jean Marc ^a   Fournier, Pierre Edouard ^a   La Scola, Bernard ^a   Drancourt, Michel ^a   Raoult, Didier ^a  

^a AIX MARSEILLE UNIVERSITY   (France)

Author keywords

Archaea; bacteria; fungi; human; MALDI TOF; mass spectrometry; microorganism; virus

Indexed keywords

AMPICILLIN; BACTERIAL TOXIN; FLUCONAZOLE;

AEROMONAS; ANAEROBIC BACTERIUM; ANTIBIOTIC RESISTANCE; ARCHAEBACTERIUM; ARTHROBACTER; BACILLUS; BACTERIUM CULTURE; BACTERIUM IDENTIFICATION; BARTONELLA; BLOOD CULTURE; CAMPYLOBACTER; CLINICAL LABORATORY; CORYNEBACTERIUM DIPHTHERIAE; COXIELLA BURNETII; ENVIRONMENT; ESCHERICHIA; FRANCISELLA TULARENSIS; FUNGUS; HAEMOPHILUS; HELICOBACTER; INTERMETHOD COMPARISON; LACTOBACILLUS; LEGIONELLA; LISTERIA; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROBIAL COLONIZATION; MYCOBACTERIUM; NEISSERIA; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; PROTISTAN COMMUNITY; PSEUDOMONAS; REVIEW; RHODOCOCCUS ERYTHROPOLIS; SALMONELLA; STAPHYLOCOCCUS; STREPTOCOCCUS; URINALYSIS; VIBRIO; VIRUS IDENTIFICATION; YERSINIA;

ARCHAEA; BACTERIA; BACTERIAL INFECTIONS; BACTERIAL TOXINS; BLOOD; ENVIRONMENTAL MICROBIOLOGY; EUKARYOTA; FUNGI; HUMANS; MICROBIAL SENSITIVITY TESTS; MICROBIOLOGICAL TECHNIQUES; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; URINE; VIRUSES;

BACTERIA (MICROORGANISMS); EUKARYOTA; FUNGI;

EID: 78650200123     PISSN: 17460913     EISSN: None     Source Type: Journal    
DOI: 10.2217/fmb.10.127     Document Type: Review

References (133)

1. Seng P, Drancourt M, Gouriet F et al.: Ongoing revolution in bacteriology: routine identifcation of bacteria by matrix-assisted laser desorption ionization time-of-fight mass spectrometry. Clin. Infect. Dis. 49(4), 543-551 (2009).
2. La Scola B, Raoult D: Direct identifcation of bacteria in positive blood culture bottles by matrix-assisted laser desorption ionisation time-of-fight mass spectrometry. PLoS One 4(11), E8041 (2009).
3. Wahl KL, Wunschel SC, Jarman KH et al.: Analysis of microbial mixtures by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry. Anal. Chem. 74(24), 6191-6199 (2002).
4. Claydon MA, Davey SN, Edwards-Jones V, Gordon DB: The rapid identifcation of intact microorganisms using mass spectrometry Nat. Biotechnol. 14(11), 1584-1586 (1996).
5. Krishnamurthy T, Ross PL: Rapid identifcation of bacteria by direct matrix-assisted laser desorption/ionization mass spectrometric analysis of whole cells. Rapid Commun. Mass Spectrom. 10(15), 1992-1996 (1996).
6. Pribil P, Fenselau C: Characterization of Enterobacteria using MALDI-TOF mass spectrometry. Anal. Chem. 77(18), 6092-6095 (2005).
7. Liu H, Du Z, Wang J, Yang R: Universal sample preparation method for characterization of bacteria by matrix-assisted laser desorption ionization-time of fight mass spectrometry. Appl. Environ. Microbiol. 73(6), 1899-1907 (2007).
8. Hsieh SY, Tseng CL, Lee YS et al.: Highly effcient classifcation and identifcation of human pathogenic bacteria by MALDI-TOF MS. Mol. Cell. Proteomics 7(2), 448-456 (2008).
9. Demirev PA, Ho YP, Ryzhov V, Fenselau C: Microorganism identifcation by mass spectrometry and protein database searches. Anal. Chem. 71(14), 2732-2738 (1999).
10. Donohue MJ, Smallwood AW, Pfaller S, Rodgers M, Shoemaker JA: The development of a matrix-assisted laser desorption/ ionization mass spectrometry-based method for the protein fngerprinting and identifcation of Aeromonas species using whole cells. J. Microbiol. Methods 65(3), 380-389 (2006).
11. Donohue MJ, Best JM, Smallwood AW, Kostich M, Rodgers M, Shoemaker JA: Differentiation of Aeromonas isolated from drinking water distribution systems using matrix-assisted laser desorption/ionization-mass spectrometry. Anal. Chem. 79(5), 1939-1946 (2007).
12. Dieckmann R, Strauch E, Alter T: Rapid identifcation and characterization of Vibrio species using whole-cell MALDI-TOF mass spectrometry. J. Appl. Microbiol. DOI: 10.11 11/j.1365-2672.2009.04647.x (2009) (Epub ahead of print).
13. Winkler MA, Uher J, Cepa S: Direct analysis and identifcation of Helicobacter and Campylobacter species by MALDI-TOF mass spectrometry. Anal. Chem. 71(16), 3416-3419 (1999).
14. Fagerquist CK, Miller WG, Harden LA et al.: Genomic and proteomic identifcation of a DNA-binding protein used in the "fngerprinting" of campylobacter species and strains by MALDI-TOF-MS protein biomarker analysis. Anal. Chem. 77(15), 4897-4907 (2005).
15. Mandrell RE, Harden LA, Bates A, Miller WG, Haddon WF, Fagerquist CK: Speciation of Campylobacter coli, C. jejuni, C. helveticus, C. lari, C. sputorum, and C. upsaliensis by matrix-assisted laser desorption ionization-time of fight mass spectrometry. Appl. Environ. Microbiol. 71(10), 6292-6307 (2005).
16. Kolinska R, Drevinek M, Jakubu V, Zemlickova H: Species identifcation of Campylobacter jejuni ssp. jejuni and C. coli by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry and PCR. Folia Microbiol. (Praha), 53(5), 403-409 (2008).
17. Alispahic M, Hummel K, Jandreski-Cvetkovic D et al.: Species-specific identifcation and differentiation of Arcobacter, Helicobacter and Campylobacter by full-spectral matrix-associated laser desorption/ionization time of fight mass spectrometry analysis. J. Med. Microbiol. 59(Pt 3), 295-301 (2010).
18. Haag AM, Taylor SN, Johnston KH, Cole RB: Rapid identifcation and speciation of Haemophilus bacteria by matrix-assisted laser desorption/ ionization time-of-fight mass spectrometry. J. Mass Spectrom. 33(8), 750-756 (1998).
19. Nilsson CL: Fingerprinting of Helicobacter pylori strains by matrix-assisted laser desorption/ionization mass spectrometric analysis. Rapid Commun. Mass Spectrom. 13(11), 1067-1071 (1999).
20. Ilina EN, Borovskaya AD, Serebryakova MV et al.: Application of matrix-assisted laser desorption/ionization time-of-fight mass spectrometry for the study of Helicobacter pylori. Rapid Commun. Mass Spectrom. 24(3), 328-334 (2010).
21. Ilina EN, Borovskaya AD, Malakhova MM et al.: Direct bacterial profling by matrix-assisted laser desorption-ionization time-of-fight mass spectrometry for identifcation of pathogenic Neisseria. J. Mol. Diagn. 11(1), 75-86 (2009).
22. Hazen TH, Martinez RJ, Chen Y et al.: Rapid identifcation of Vibrio parahaemolyticus by whole-cell matrix-assisted laser desorption ionization-time of fight mass spectrometry. Appl. Environ. Microbiol. 75(21), 6745-6756 (2009).
23. Mazzeo MF, Sorrentino A, Gaita M et al.: Matrix-assisted laser desorption ionization-time of fight mass spectrometry for the discrimination of food-borne microorganisms. Appl. Environ. Microbiol. 72(2), 1180-1189 (2006).
24. Teramoto K, Sato H, Sun L et al.: Phylogenetic classifcation of Pseudomonas putida strains by MALDI-MS using ribosomal subunit proteins as biomarkers. Anal. Chem. 79(22), 8712-8719 (2007).
25. Mellmann A, Cloud J, Maier T et al.: Evaluation of matrix-assisted laser desorption ionization-time-of-fight mass spectrometry in comparison to 16S rRNA gene sequencing for species identifcation of nonfermenting bacteria. J. Clin. Microbiol. 46(6), 1946-1954 (2008).
26. Mellmann A, Bimet F, Bizet C et al.: High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of fight mass spectrometry-based species identifcation of nonfermenting bacteria. J. Clin. Microbiol. 47(11), 3732-3734 (2009).
27. Degand N, Carbonnelle E, Dauphin B et al.: Matrix-assisted laser desorption ionization-time of fight mass spectrometry for identifcation of nonfermenting Gram-negative bacilli isolated from cystic fibrosis patients. J. Clin. Microbiol. 46(10), 3361-3367 (2008).
28. Vanlaere E, Sergeant K, Dawyndt P et al.: Matrix-assisted laser desorption ionisation-time-of of-fight mass spectrometry of intact cells allows rapid identifcation of Burkholderia cepacia complex. J. Microbiol. Methods 75(2), 279-286 (2008).
29. Fournier PE, Couderc C, Buffet S, Flaudrops C, Raoult D: Rapid and cost-effective identifcation of Bartonella species using mass spectrometry. J. Med. Microbiol. 58(Pt 9), 1154-1159 (2009).
30. Shaw EI, Moura H, Woolftt AR, Ospina M, Thompson HA, Barr JR: Identifcation of biomarkers of whole Coxiella burnetii phase I by MALDI-TOF mass spectrometry. Anal. Chem. 76(14), 4017-4022 (2004).
31. Pierce CY, Barr JR, Woolftt AR et al.: Strain and phase identifcation of the U.S. category B agent Coxiella burnetii by matrix assisted laser desorption/ionization time-of-fight mass spectrometry and multivariate pattern recognition. Anal. Chim. Acta 583(1), 23-31 (2007).
32. Moliner C, Ginevra C, Jarraud S et al.: Rapid identifcation of Legionella species by mass spectrometry. J. Med. Microbiol. 59(Pt 3), 273-284 (2010).
33. Fujinami Y, Kikkawa HS, Kurosaki Y, Sakurada K, Yoshino M, Yasuda J: Rapid discrimination of Legionella by matrix-assisted laser desorption ionization time-of-fight mass spectrometry. Microbiol. Res. DOI: 10.1016/j.micres.2010.02.005 (2010) (Epub ahead of print).
34. Vargha M, Takats Z, Konopka A, Nakatsu CH: Optimization of MALDI-TOF MS for strain level differentiation of Arthrobacter isolates. J. Microbiol. Methods 66(3), 399-409 (2006).
35. Sun L, Teramoto K, Sato H, Torimura M, Tao H, Shintani T: Characterization of ribosomal proteins as biomarkers for matrix-assisted laser desorption/ionization mass spectral identifcation of Lactobacillus plantarum. Rapid Commun. Mass Spectrom. 20(24), 3789-3798 (2006).
36. Barbuddhe SB, Maier T, Schwarz G et al.: Rapid identifcation and typing of listeria species by matrix-assisted laser desorption ionization-time of fight mass spectrometry. Appl. Environ. Microbiol. 74(17), 5402-5407 (2008).
37. Carbonnelle E, Beretti JL, Cottyn S et al.: Rapid identifcation of staphylococci isolated in clinical microbiology laboratories by matrix-assisted laser desorption ionization-time of fight mass spectrometry. J. Clin. Microbiol. 45(7), 2156-2161 (2007).
38. Dubois D, Leyssene D, Chacornac JP et al.: Identifcation of a variety of Staphylococcus species by matrix-assisted laser desorption ionization-time of fight mass spectrometry. J. Clin. Microbiol. 48(3), 941-945 (2010).
39. Rajakaruna L, Hallas G, Molenaar L et al.: High throughput identifcation of clinical isolates of Staphylococcus aureus using MALDI-TOF-MS of intact cells. Infect. Genet. Evol. 9(4), 507-513 (2009).
40. Dupont C, Sivadon-Tardy V, Bille E et al.: Identifcation of clinical coagulase-negative staphylococci, isolated in microbiology laboratories, by matrix-assisted laser desorption/ionization-time of fight mass spectrometry and two automated systems. Clin. Microbiol. Infect. 16(7), 998-1004 (2009).
41. Spanu T, De Carolis E, Fiori B et al.: Evaluation of matrix-assisted laser desorption ionization-time-of-fight mass spectrometry in comparison to rpoB gene sequencing for species identifcation of bloodstream infection staphylococcal isolates. Clin. Microbiol. Infect. DOI: 10.1111/j.1469-0691.2010. 03181.x (2010) (Epub ahead of print).
42. Friedrichs C, Rodloff AC, Chhatwal GS, Schellenberger W, Eschrich K: Rapid identifcation of viridans streptococci by mass spectrometric discrimination. J. Clin. Microbiol. 45(8), 2392-2397 (2007).
43. Rupf S, Breitung K, Schellenberger W, Merte K, Kneist S, Eschrich K: Differentiation of mutans streptococci by intact cell matrix-assisted laser desorption/ionization time-of-fight mass spectrometry. Oral Microbiol. Immunol. 20(5), 267-273 (2005).
44. Blondiaux N, Gaillot O, Courcol RJ: [MALDI-TOF mass spectrometry to identify clinical bacterial isolates: Evaluation in a teaching hospital.]. Pathol. Biol. (Paris) 58(1), 55-57 (2010).
45. van Veen SQ, Claas EC, Kuijper EJ: High-throughput identifcation of bacteria and yeast by matrix-assisted laser desorption ionization mass spectrometry (MALDI-TOF MS) in routine medical microbiology laboratory. J. Clin. Microbiol. DOI: 10.1128/JCM.02071-09 (2010) (Epub ahead of print).
46. Eigner U, Holfelder M, Oberdorfer K, Betz-Wild U, Bertsch D, Fahr AM: Performance of a matrix-assisted laser desorption ionization-time-of-fight mass spectrometry system for the identifcation of bacterial isolates in the clinical routine laboratory. Clin. Lab. 55(7-8), 289-296 (2009).
47. Bittar CC, Bosdure E, Stremler N et al.: Outbreak of Corynebacterium pseudodiphtheriticum in cystic fibrosis patients: a paradigm of MALDI-TOF MS as a new tool for identifcation of emerging pathogens. Emerg. Infect. Dis. J. (2010) (In Press).
48. Hettick JM, Kashon ML, Simpson JP, Siegel PD, Mazurek GH, Weissman DN: Proteomic profling of intact mycobacteria by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry. Anal. Chem. 76(19), 5769-5776 (2004).
49. Pignone M, Greth KM, Cooper J, Emerson D, Tang J: Identifcation of mycobacteria by matrix-assisted laser desorption ionization-time-of-fight mass spectrometry. J. Clin. Microbiol. 44(6), 1963-1970 (2006).
50. Hettick JM, Kashon ML, Slaven JE et al.: Discrimination of intact mycobacteria at the strain level: a combined MALDI-TOF MS and biostatistical analysis. Proteomics 6(24), 6416-6425 (2006).
51. Shah HN, Keys CJ, Schmid O, Gharbia SE: Matrix-assisted laser desorption/ionization time-of-fight mass spectrometry and proteomics: a new era in anaerobic microbiology. Clin. Infect. Dis. 35(Suppl. 1), S58-S64 (2002).
52. Stingu CS, Rodloff AC, Jentsch H, Schaumann R, Eschrich K: Rapid identifcation of oral anaerobic bacteria cultivated from subgingival bioflm by MALDI-TOF-MS. Oral Microbiol. Immunol. 23 (5), 372-376 (2008).
53. Nagy E, Maier T, Urban E, Terhes G, Kostrzewa M: Species identifcation of clinical isolates of Bacteroides by matrix-assisted laser-desorption/ionization time-of-fight mass spectrometry Clin. Microbiol. Infect. 15(8), 796-802 (2009).
54. Grosse-Herrenthey A, Maier T, Gessler F et al.: Challenging the problem of clostridial identifcation with matrix-assisted laser desorption and ionization-time-of-fight mass spectrometry (MALDI-TOF MS). Anaerobe 14(4), 242-249 (2008).
55. Fastner J, Erhard M, von Dohren H: Determination of oligopeptide diversity within a natural population of Microcystis spp. (cyanobacteria) by typing single colonies by matrix-assisted laser desorption ionization-time of fight mass spectrometry Appl. Environ. Microbiol. 67(11), 5069-5076 (2001).
56. Cayrou C, Drancourt M: Matrix-assisted laser desorption/ionization time-of-fight mass spectrometry for the identifcation of environmental organisms: the Planctomycetes paradigm. Environment. Microbiol. DOI: 10.1111/j. 1758-2229.2010.00176.x (2010) (Epub ahead of print).
57. Lynn EC, Chung MC, Tsai WC, Han CC: Identifcation of Enterobacteriaceae bacteria by direct matrix-assisted laser desorptiom/ ionization mass spectrometric analysis of whole cells. Rapid Commun. Mass Spectrom. 13(20), 2022-2027 (1999).
58. Leuschner RG, Beresford-Jones N, Robinson C: Difference and consensus of whole cell Salmonella enterica subsp. enterica serovars matrix-assisted laser desorption/ ionization time-of-fight mass spectrometry spectra. Lett. Appl. Microbiol. 38(1), 24-31 (2004).
59. Dieckmann R, Helmuth R, Erhard M, Malorny B: Rapid classifcation and identifcation of Salmonellae at the species and subspecies levels by whole-cell matrix-assisted laser desorption ionization-time of fight mass spectrometry. Appl. Environ. Microbiol. 74(24), 7767-7778 (2008).
60. Ochoa ML, Harrington PB: Immunomagnetic isolation of enterohemorrhagic Escherichia coli O157:H7 from ground beef and identifcation by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry and database searches. Anal. Chem. 77(16), 5258-5267 (2005).
61. Lartigue MF, Hery-Arnaud G, Haguenoer E et al.: Identifcation of Streptococcus agalactiae isolates from various phylogenetic lineages by matrix-assisted laser desorption ionization-time of fight mass spectrometry. J. Clin. Microbiol. 47(7), 2284-2287 (2009).
62. Williamson YM, Moura H, Woolftt AR et al.: Differentiation of Streptococcus pneumoniae conjunctivitis outbreak isolates by matrix-assisted laser desorption ionization-time of fight mass spectrometry. Appl. Environ. Microbiol. 74(19), 5891-5897 (2008).
63. Kumar MP, Vairamani M, Raju RP et al.: Rapid discrimination between strains of b haemolytic streptococci by intact cell mass spectrometry. Indian J. Med. Res. 119(6), 283-288 (2004).
64. Moura H, Woolftt AR, Carvalho MG et al.: MALDI-TOF mass spectrometry as a tool for differentiation of invasive and noninvasive Streptococcus pyogenes isolates. FEMS Immunol. Med. Microbiol. 53(3), 333-342 (2008).
65. Krishnamurthy T, Ross PL, Rajamani U: Detection of pathogenic and non-pathogenic bacteria by matrix-assisted laser desorption/ ionization time-of-fight mass spectrometry. Rapid Commun. Mass Spectrom. 10(8), 883-888 (1996).
66. Ryzhov V, Bundy JL, Fenselau C, Taranenko N, Doroshenko V, Prasad CR: Matrix-assisted laser desorption/ionization time-of-fight analysis of Bacillus spores using a 2.94 microm infrared laser. Rapid Commun. Mass Spectrom. 14(18), 1701-1706 (2000).
67. Ryzhov V, Hathout Y, Fenselau C: Rapid characterization of spores of Bacillus cereus group bacteria by matrix-assisted laser desorption-ionization time-of-fight mass spectrometry. Appl. Environ. Microbiol. 66(9), 3828-3834 (2000).
68. Elhanany E, Barak R, Fisher M, Kobiler D, Altboum Z: Detection of specific Bacillus anthracis spore biomarkers by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry. Rapid Commun. Mass Spectrom. 15(22), 2110-2116 (2001).
69. Demirev PA, Fenselau C: Mass spectrometry in biodefense. J. Mass Spectrom. 43(11), 1441-1457 (2008).
70. Lasch P, Beyer W, Nattermann H et al.: Identifcation of Bacillus anthracis by using matrix-assisted laser desorption ionization-time of fight mass spectrometry and artifcial neural networks. Appl. Environ. Microbiol. 75(22), 7229-7242 (2009).
71. Seibold E, Maier T, Kostrzewa M, Zeman E, Splettstoesser W: Identifcation of Francisella tularensis by whole-cell matrix-assisted laser desorption ionization-time of fight mass spectrometry: fast, reliable, robust, and cost-effective differentiation on species and subspecies levels. J. Clin. Microbiol. 48(4), 1061-1069 (2010).
72. Teramoto K, Kitagawa W, Sato H, Torimura M, Tamura T, Tao H: Phylogenetic analysis of Rhodococcus erythropolis based on the variation of ribosomal proteins as observed by matrix-assisted laser desorption ionization-mass spectrometry without using genome information. J. Biosci. Bioeng. 108(4), 348-353 (2009).
73. Bernardo K, Fleer S, Pakulat N, Krut O, Hunger F, Kronke M: Identifcation of Staphylococcus aureus exotoxins by combined sodium dodecyl sulfate gel electrophoresis and matrix-assisted laser desorption/ ionization-time of fight mass spectrometry. Proteomics 2(6), 740-746 (2002).
74. Bittar F, Ouchenane Z, Smati F, Raoult D, Rolain JM: MALDI-TOF-MS for rapid detection of staphylococcal Panton-Valentine leukocidin. Int. J. Antimicrob. Agents 34(5), 467-470 (2009).
75. Edwards-Jones V, Claydon MA, Evason DJ, Walker J, Fox AJ, Gordon DB: Rapid discrimination between methicillin-sensitive and methicillin-resistant Staphylococcus aureus by intact cell mass spectrometry. J. Med. Microbiol. 49(3), 295-300 (2000).
76. Walker J, Fox AJ, Edwards-Jones V, Gordon DB: Intact cell mass spectrometry (ICMS) used to type methicillin-resistant Staphylococcus aureus: media effects and inter-laboratory reproducibility. J. Microbiol. Methods 48(2-3), 117-126 (2002).
77. Jackson KA, Edwards-Jones V, Sutton CW, Fox AJ: Optimisation of intact cell MALDI method for fngerprinting of methicillin-resistant Staphylococcus aureus. J. Microbiol. Methods 62(3), 273-284 (2005).
78. Du Z, Yang R, Guo Z, Song Y, Wang J: Identifcation of Staphylococcus aureus and determination of its methicillin resistance by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry. Anal. Chem. 74(21), 5487-5491 (2002).
79. Bernardo K, Pakulat N, Macht M et al.: Identifcation and discrimination of Staphylococcus aureus strains using matrix-assisted laser desorption/ionization-time of fight mass spectrometry. Proteomics 2(6), 747-753 (2002). (Pubitemid 34693577)
80. Majcherczyk PA, McKenna T, Moreillon P, Vaudaux P: The discriminatory power of MALDI-TOF mass spectrometry to differentiate between isogenic teicoplanin-susceptible and teicoplanin-resistant strains of methicillin-resistant Staphylococcus aureus. FEMS Microbiol. Lett. 255(2), 233-239 (2006).
81. Camara JE, Hays FA: Discrimination between wild-type and ampicillin-resistant Escherichia coli by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry. Anal. Bioanal. Chem. 389(5), 1633-1638 (2007).
82. Russell SC, Edwards N, Fenselau C: Detection of plasmid insertion in Escherichia coli by MALDI-TOF mass spectrometry. Anal. Chem. 79(14), 5399-5406 (2007).
83. Marinach C, Alanio A, Palous M et al.: MALDI-TOF MS-based drug susceptibility testing of pathogens: the example of Candida albicans and fuconazole. Proteomics 9(20), 4627-4631 (2009).
84. Krader P, Emerson D: Identifcation of archaea and some extremophilic bacteria using matrix-assisted laser desorption/ ionization time-of-fight (MALDI-TOF) mass spectrometry. Extremophiles 8(4), 259-268 (2004).
85. Welham KJ, Domin MA, Johnson K, Jones L, Ashton DS: Characterization of fungal spores by laser desorption/ionization time-of-fight mass spectrometry. Rapid Commun. Mass Spectrom. 14(5), 307-310 (2000).
86. Chen HY, Chen YC: Characterization of intact Penicillium spores by matrix-assisted laser desorption/ionization mass spectrometry. Rapid Commun. Mass Spectrom. 19(23), 3564-3568 (2005).
87. Hettick JM, Green BJ, Buskirk AD et al.: Discrimination of Penicillium isolates by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry fngerprinting. Rapid Commun. Mass Spectrom. 22(16), 2555-2560 (2008).
88. Li TY, Liu BH, Chen YC: Characterization of Aspergillus spores by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry. Rapid Commun. Mass Spectrom. 14(24), 2393-2400 (2000).
89. Hettick JM, Green BJ, Buskirk AD et al.: Discrimination of Aspergillus isolates at the species and strain level by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry fngerprinting. Anal. Biochem. 380(2), 276-281 (2008).
90. Sulc M, Peslova K, Zabka M, Hajduch M, Havlicek V: Biomarkers of Aspergillus spores: strain typing and protein identifcation. Int. J. Mass Spectrom. 280(1-3), 162-168 (2009).
91. Marinach-Patrice C, Lethuillier A, Marly A et al.: Use of mass spectrometry to identify clinical Fusarium isolates. Clin. Microbiol. Infect. 15(7), 634-642 (2009).
92. Dong H, Kemptner J, Marchetti-Deschmann M, Kubicek CP, Allmaier G: Development of a MALDI two-layer volume sample preparation technique for analysis of colored conidia spores of Fusarium by MALDI linear TOF mass spectrometry. Anal. Bioanal. Chem. 395(5), 1373-1383 (2009).
93. Kemptner J, Marchetti-Deschmann M, Mach R, Druzhinina IS, Kubicek CP, Allmaier G: Evaluation of matrix-assisted laser desorption/ionization (MALDI) preparation techniques for surface characterization of intact Fusarium spores by MALDI linear time-of-fight mass spectrometry. Rapid Commun. Mass Spectrom. 23(6), 877-884 (2009).
94. Amiri-Eliasi B, Fenselau C: Characterization of protein biomarkers desorbed by MALDI from whole fungal cells. Anal. Chem. 73(21), 5228-5231 (2001). (Pubitemid 33032241)
95. Moura H, Ospina M, Woolftt AR, Barr JR, Visvesvara GS: Analysis of four human microsporidian isolates by MALDI-TOF mass spectrometry. J. Eukaryot. Microbiol. 50(3), 156-163 (2003).
96. Neuhof T, Dieckmann R, Druzhinina IS, Kubicek CP, von Dohren H: Intact-cell MALDI-TOF mass spectrometry analysis of peptaibol formation by the genus Trichoderma/Hypocrea: can molecular phylogeny of species predict peptaibol structures? Microbiology 153(Pt 10), 3417-3437 (2007).
97. Neuhof T, Dieckmann R, Druzhinina IS et al.: Direct identifcation of hydrophobins and their processing in Trichoderma using intact-cell MALDI-TOF MS. FEBS J. 274(3), 841-852 (2007).
98. Degenkolb T, von Dohren H, Nielsen KF, Samuels GJ, Bruckner H: Recent advances and future prospects in peptaibiotics, hydrophobin, and mycotoxin research, and their importance for chemotaxonomy of Trichoderma and Hypocrea. Chem. Biodivers. 5(5), 671-680 (2008).
99. Marklein G, Josten M, Klanke U et al.: Matrix-assisted laser desorption ionization-time of fight mass spectrometry for fast and reliable identifcation of clinical yeast isolates. J. Clin. Microbiol. 47(9), 2912-2917 (2009).
100. Qian J, Cutler JE, Cole RB, Cai Y: MALDI-TOF mass signatures for differentiation of yeast species, strain grouping and monitoring of morphogenesis markers. Anal. Bioanal. Chem. 392(3), 439-449 (2008).
101. Valentine NB, Wahl JH, Kingsley MT, Wahl KL: Direct surface analysis of fungal species by matrix-assisted laser desorption/ ionization mass spectrometry. Rapid Commun. Mass Spectrom. 16(14), 1352-1357 (2002).
102. Santos C, Paterson RR, Venancio A, Lima N: Filamentous fungal characterizations by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry. J. Appl. Microbiol. 108(2), 375-385 (2009).
103. Marinach-Patrice C, Fekkar A, Atanasova R et al.: Rapid species diagnosis for invasive candidiasis using mass spectrometry. PLoS One 5(1), e8862 (2010).
104. Ferroni A, Suarez S, Beretti JL et al.: Real time identifcation of bacteria and yeast in positive blood culture broths by MALDI-TOF-mass spectrometry. J. Clin. Microbiol. DOI: 10.1128/ JCM.02485-09 (2010) (Epub ahead of print).
105. Pfohler C, Hollemeyer K, Heinzle E et al.: Matrix-assisted laser desorption/ionization time-of-fight mass spectrometry: a new tool in diagnostic investigation of nail disorders? Exp. Dermatol. 18(10), 880-882 (2009).
106. Sharma S, Sharma SK, Modak R, Karmodiya K, Surolia N, Surolia A: Mass spectrometry-based systems approach for identifcation of inhibitors of Plasmodium falciparum fatty acid synthase. Antimicrob. Agents Chemother. 51(7), 2552-2558 (2007).
107. Marks F, Meyer CG, Sievertsen J et al.: Genotyping of Plasmodium falciparum pyrimethamine resistance by matrix-assisted laser desorption-ionization time-of-fight mass spectrometry. Antimicrob. Agents Chemother. 48(2), 466-472 (2004).
108. Liu L, Xu L, Yan F, Yan R, Song X, Li X: Immunoproteomic analysis of the second-generation merozoite proteins of Eimeria tenella. Vet. Parasitol. 164(2-4), 173-182 (2009).
109. Dea-Ayuela MA, Rama-Iniguez S, Bolas-Fernandez F: Proteomic analysis of antigens from Leishmania infantum promastigotes. Proteomics 6(14), 4187-4194 (2006).
110. Makioka A, Kumagai M, Kobayashi S, Takeuchi T: Differences in protein profles of the isolates of Entamoeba histolytica and E. dispar by surface-enhanced laser desorption ionization time-of-fight mass spectrometry (SELDI-TOF MS) ProteinChip assays. Parasitol. Res. 102(1), 103-110 (2007).
111. Agranoff D, Stich A, Abel P, Krishna S: Proteomic fngerprinting for the diagnosis of human African trypanosomiasis. Trends Parasitol. 21(4), 154-157 (2005).
112. Papadopoulos MC, Abel PM, Agranoff D et al.: A novel and accurate diagnostic test for human African trypanosomiasis. Lancet 363(9418), 1358-1363 (2004).
113. Zhang X, Scalf M, Berggren TW, Westphall MS, Smith LM: Identifcation of mammalian cell lines using MALDI-TOF and LC-ESI-MS/MS mass spectrometry. J. Am. Soc. Mass Spectrom. 17(4), 490-499 (2006).
114. Karger A, Bettin B, Lenk M, Mettenleiter TC: Rapid characterisation of cell cultures by matrix-assisted laser desorption/ionisation mass spectrometric typing. J. Virol. Methods 164(1-2), 116-121 (2010).
115. Lopaticki S, Morrow CJ, Gorman JJ: Characterization of pathotype-specific epitopes of newcastle disease virus fusion glycoproteins by matrix-assisted laser desorption/ionization time-of-fight mass spectrometry and post-source decay sequencing. J. Mass Spectrom. 33(10), 950-960 (1998).
116. Kim YJ, Freas A, Fenselau C: Analysis of viral glycoproteins by MALDI-TOF mass spectrometry. Anal. Chem. 73(7), 1544-1548 (2001).
117. Colquhoun DR, Schwab KJ, Cole RN, Halden RU: Detection of norovirus capsid protein in authentic standards and in stool extracts by matrix-assisted laser desorption ionization and nanospray mass spectrometry. Appl. Environ. Microbiol. 72(4), 2749-2755 (2006).
118. Yao ZP, Demirev PA, Fenselau C: Mass spectrometry-based proteolytic mapping for rapid virus identifcation. Anal. Chem. 74(11), 2529-2534 (2002).
119. Luan J, Yuan J, Li X et al.: Multiplex detection of 60 hepatitis B virus variants by MALDI-TOF mass spectrometry. Clin. Chem. 55(8), 1503-1509 (2009).
120. Ilina EN, Malakhova MV, Generozov EV, Nikolaev EN, Govorun VM: Matrix-assisted laser desorption ionization-time of fight (mass spectrometry) for hepatitis C virus genotyping. J. Clin. Microbiol. 43(6), 2810-2815 (2005).
121. Michael K, Harder TC, Mettenleiter TC, Karger A: Diagnosis and strain differentiation of avian infuenza viruses by restriction fragment mass analysis. J. Virol. Methods 158(1-2), 63-69 (2009).
122. La Scola B, Campocasso A, N'Dong R et al.: Tentative characterization of new environmental giant viruses by MALDI-TOF mass spectrometry. Intervirology 53 (5), 344-353 (2010).
123. Valentine N, Wunschel S, Wunschel D, Petersen C, Wahl K: Effect of culture conditions on microorganism identifcation by matrix-assisted laser desorption ionization mass spectrometry. Appl. Environ. Microbiol. 71(1), 58-64 (2005).
124. Wunschel SC, Jarman KH, Petersen CE et al.: Bacterial analysis by MALDI-TOF mass spectrometry: an inter-laboratory comparison. J. Am. Soc. Mass Spectrom. 16(4), 456-462 (2005).
125. Szabados F, Woloszyn J, Richter C, Kaase M, Gatermann SG: A Biotyper 2.0 (Bruker Daltonics)-based score cut-off of actually 2 is superior to molecular Staphylococcus aureus identifcation. J. Med. Microbiol. DOI: 10.1099/jmm.0.016733-0 (2010) (Epub ahead of print).
126. Anzai Y, Kim H, Park JY, Wakabayashi H, Oyaizu H: Phylogenetic affliation of the pseudomonads based on 16S rRNA sequence. Int. J. Syst. Evol. Microbiol. 50(Pt 4), 1563-1589 (2000).
127. Bizzini A, Durussel C, Bille J, Greub G, Prod'hom G: Performance of matrix-assisted laser desorption/ionization time-of-fight mass spectrometry for the identifcation of bacterial strains routinely isolated in a clinical microbiology laboratory. J. Clin. Microbiol. 48(5), 1549-1554 (2010).
128. Cherkaoui A, Hibbs J, Emonet S et al.: Comparison of two matrix-assisted laser desorption ionization - time of fight mass spectrometry methods with conventional phenotypic identifcation for routine bacterial speciation. J. Clin. Microbiol. DOI: 10.1128/ JCM.01881-09 (2010) (Epub ahead of print).
129. Stevenson LG, Drake SK, Murray PR: Rapid identifcation of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of fight mass spectrometry. J. Clin. Microbiol. 48(2), 444-447 (2010).
130. Christner M, Rohde H, Wolters M, Sobottka I, Wegscheider K, Aepfelbacher M: Rapid identifcation of bacteria from positive blood culture bottles by use of matrix-assisted laser desorption-ionization time of fight mass spectrometry fngerprinting. J. Clin. Microbiol. 48(5), 1584-1591 (2010).
131. Szabados F, Michels M, Kaase M, Gatermann S: The sensitivity of direct identifcation from positive BacT/ALERT(TM) (BioMerieux) blood culture bottles by MALDI-TOF mass spectrometry is low. Clin. Microbiol. Infect. (2010).
132. Ferreira L, Sanchez-Juanes F, Gonzalez-Avila M et al.: Direct identifcation of urinary tract pathogens from urine samples by MALDI-TOF (matrix-assisted laser desorption ionization time-of-fight) mass spectrometry. J. Clin. Microbiol. 48(6), 2110-2115 (2010) (Epub ahead of print).
133. Parisi D, Magliulo M, Nanni P, Casale M, Forina M, Roda A: Analysis and classifcation of bacteria by matrix-assisted laser desorption/ ionization time-of-fight mass spectrometry and a chemometric approach. Anal. Bioanal. Chem. 391(6), 2127-2134 (2008).