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Volumn 3, Issue 4, 2010, Pages 304-312

Statistical Evaluation of Real-Time PCR Protocols Applied to Quantify Genetically Modified Maize

Author keywords

Certified Reference Materials (CRMs); Genetically Modified Organisms (GMOs); GMO Copy Number; MON810

Indexed keywords

CALIBRANTS; CERTIFIED REFERENCE MATERIALS; COPY NUMBER; CT-VALUE; DNA QUANTIFICATION METHOD; EVENT-SPECIFIC; EXPERIMENTAL DESIGN; GENETICALLY MODIFIED MAIZE; GENETICALLY MODIFIED ORGANISMS; GENETICALLY MODIFIED ORGANISMS (GMOS); GENOMIC DNA; GM MAIZE; INTERACTION EFFECT; MON810; REAL-TIME PCR; SEVERAL VARIABLES; STATISTICAL EVALUATION; STATISTICALLY SIGNIFICANT DIFFERENCE;

EID: 78650179839     PISSN: 19369751     EISSN: 1936976X     Source Type: Journal    
DOI: 10.1007/s12161-010-9135-7     Document Type: Article
Times cited : (6)

References (34)
  • 3
    • 33748793124 scopus 로고    scopus 로고
    • DNA-extraction: important step of quantitative detection of GMO in food matrixes
    • Brodmann P (2002) DNA-extraction: important step of quantitative detection of GMO in food matrixes. New Food 1: 57-61.
    • (2002) New Food , vol.1 , pp. 57-61
    • Brodmann, P.1
  • 4
    • 54349122579 scopus 로고    scopus 로고
    • A single nucleotide polymorphism (SNP839) in the adh1 reference gene affects the quantitation of genetically modified maize (Zea mays L.)
    • Broothaerts W, Corbisier P, Schimmel H, Trapmann S, Vincent S, Emons H (2008) A single nucleotide polymorphism (SNP839) in the adh1 reference gene affects the quantitation of genetically modified maize (Zea mays L.). J Agric Food Chem 56(19): 8825-8831.
    • (2008) J Agric Food Chem , vol.56 , Issue.19 , pp. 8825-8831
    • Broothaerts, W.1    Corbisier, P.2    Schimmel, H.3    Trapmann, S.4    Vincent, S.5    Emons, H.6
  • 5
    • 16544366468 scopus 로고    scopus 로고
    • Analysis and interpretation of data from Real-Time PCR trace detection methods using quantification of GM soya as a model system
    • Burns MJ, Valdivia H, Harris N (2004) Analysis and interpretation of data from Real-Time PCR trace detection methods using quantification of GM soya as a model system. Anal Bioanal Chem 387: 1616-1623.
    • (2004) Anal Bioanal Chem , vol.387 , pp. 1616-1623
    • Burns, M.J.1    Valdivia, H.2    Harris, N.3
  • 6
    • 29244438450 scopus 로고    scopus 로고
    • Standardization of data from real-time quantitative PCR methods - evaluation of outliers and comparison of calibration curves
    • Burns MJ, Nixon GJ, Foy CA, Harris N (2005) Standardization of data from real-time quantitative PCR methods - evaluation of outliers and comparison of calibration curves. BMC Biotechnol 7: 5-31.
    • (2005) BMC Biotechnol , vol.7 , pp. 5-31
    • Burns, M.J.1    Nixon, G.J.2    Foy, C.A.3    Harris, N.4
  • 9
    • 33748798872 scopus 로고    scopus 로고
    • Critical points of DNA quantification by Real-Time PCR - effects of DNA extraction method and sample matrix on quantification of genetically modified organisms
    • Cankar K, Štebih D, Dreo T, Žel J, Gruden K (2006) Critical points of DNA quantification by Real-Time PCR - effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnol 6: 37.
    • (2006) BMC Biotechnol , vol.6 , pp. 37
    • Cankar, K.1    Štebih, D.2    Dreo, T.3    Žel, J.4    Gruden, K.5
  • 10
    • 38349065355 scopus 로고    scopus 로고
    • A strategy for designing multi-taxa specific reference gene systems. Example of application--ppi Phosphofructokinase (ppi-PPF) used for the detection and quantification of three taxa: maize (Zea mays), cotton (Gossypium hirsutum) and rice (Oryza sativa)
    • Chaouachi M, Giancola S, Romaniuk M, Laval V, Bertheau Y, Brunel D (2007) A strategy for designing multi-taxa specific reference gene systems. Example of application--ppi Phosphofructokinase (ppi-PPF) used for the detection and quantification of three taxa: maize (Zea mays), cotton (Gossypium hirsutum) and rice (Oryza sativa). J Agric Food Chem 55(20): 8003-8010.
    • (2007) J Agric Food Chem , vol.55 , Issue.20 , pp. 8003-8010
    • Chaouachi, M.1    Giancola, S.2    Romaniuk, M.3    Laval, V.4    Bertheau, Y.5    Brunel, D.6
  • 11
    • 34249327757 scopus 로고    scopus 로고
    • Toward metrological traceability for DNA fragment ratios in GM quantification. 3. Suitability of DNA calibrants studied with a MON 810 corn model
    • Charels D, Broeders S, Corbisier P, Trapmann S, Schimmel H, Emons H (2007) Toward metrological traceability for DNA fragment ratios in GM quantification. 3. Suitability of DNA calibrants studied with a MON 810 corn model. J Agric Food Chem 55(9): 3268-3274.
    • (2007) J Agric Food Chem , vol.55 , Issue.9 , pp. 3268-3274
    • Charels, D.1    Broeders, S.2    Corbisier, P.3    Trapmann, S.4    Schimmel, H.5    Emons, H.6
  • 12
    • 2042526375 scopus 로고    scopus 로고
    • Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed
    • European Commission
    • European Commission (2003a) Regulation (EC) No 1829/2003 of the European Parliament and of the Council of 22 September 2003 on genetically modified food and feed. Official Journal L 268: 0001-0023.
    • (2003) Official Journal , vol.L 268 , pp. 0001-0023
  • 13
    • 2342584460 scopus 로고    scopus 로고
    • Regulation (EC) 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labeling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC
    • European Commission
    • European Commission (2003b) Regulation (EC) 1830/2003 of the European Parliament and of the Council of 22 September 2003 concerning the traceability and labeling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive 2001/18/EC. Official Journal L 268: 0024-0028.
    • (2003) Official Journal , vol.L 268 , pp. 0024-0028
  • 14
    • 17144425121 scopus 로고    scopus 로고
    • Recommendation (EC) No 787/2004 of the European Parliament and of the Council of 4 October 2004 on technical guidance for sampling and detection of genetically modified organisms and material produced from genetically modified organisms as or in products in the context of Regulation (EC) No 1830/2003
    • European Commission
    • European Commission (2004) Recommendation (EC) No 787/2004 of the European Parliament and of the Council of 4 October 2004 on technical guidance for sampling and detection of genetically modified organisms and material produced from genetically modified organisms as or in products in the context of Regulation (EC) No 1830/2003. Official Journal L 348: 0018-0026.
    • (2004) Official Journal , vol.L 348 , pp. 0018-0026
  • 15
    • 78650179801 scopus 로고    scopus 로고
    • Gent Vakgroep Moleculaire Biologie Universiteit, Belgium: Gent Vakgroep Moleculaire Biologie Universiteit, Division of Biosafety and Biotechnology (SPHI)
    • Gent Vakgroep Moleculaire Biologie Universiteit (2005) Accession number LMBP 5095, pENGL-03-012, SD 05-51. Gent Vakgroep Moleculaire Biologie Universiteit, Division of Biosafety and Biotechnology (SPHI), Belgium.
    • (2005) Accession Number LMBP 5095, PENGL-03-012, SD 05-51
  • 16
    • 0346009307 scopus 로고    scopus 로고
    • Development of Real-Time PCR systems based on SYBR® Green I, Amplifluor™ and Taqman® technologies for specific quantitative detection of the transgenic maize event GA 21
    • Hernandez M, Esteve T, Prat S, Pla M (2004) Development of Real-Time PCR systems based on SYBR® Green I, Amplifluor™ and Taqman® technologies for specific quantitative detection of the transgenic maize event GA 21. J Cer Sci 39(1): 99-107.
    • (2004) J Cer Sci , vol.39 , Issue.1 , pp. 99-107
    • Hernandez, M.1    Esteve, T.2    Prat, S.3    Pla, M.4
  • 17
    • 3242776821 scopus 로고    scopus 로고
    • Development and Comparison of Four Real-Time Polymerase Chain Reaction Systems for Specific Detection and Quantification of Zea mays L
    • Hernández M, Duplan MN, Berthier G, Vaïtilingom M, Hauser W, Freyer R, Pla M, Bertheau Y (2004) Development and Comparison of Four Real-Time Polymerase Chain Reaction Systems for Specific Detection and Quantification of Zea mays L. J Agric Food Chem 52(15): 4632-4637.
    • (2004) J Agric Food Chem , vol.52 , Issue.15 , pp. 4632-4637
    • Hernández, M.1    Duplan, M.N.2    Berthier, G.3    Vaïtilingom, M.4    Hauser, W.5    Freyer, R.6    Pla, M.7    Bertheau, Y.8
  • 18
    • 25444460535 scopus 로고    scopus 로고
    • Current methodology for detection, identification and quantification of genetically modified organisms
    • Hernández M, Rodríguez-Lázaro D, Ferrando A (2005) Current methodology for detection, identification and quantification of genetically modified organisms. Current Anal Chem 1: 203-221.
    • (2005) Current Anal Chem , vol.1 , pp. 203-221
    • Hernández, M.1    Rodríguez-Lázaro, D.2    Ferrando, A.3
  • 19
  • 20
    • 0038167336 scopus 로고    scopus 로고
    • 5′-Nuclease PCR for quantitation event-specific detection of the genetically modified Mon 810 MaisGard maize
    • Holk A, Va-LucDiderjerjean M, Rudi K (2002) 5′-Nuclease PCR for quantitation event-specific detection of the genetically modified Mon 810 MaisGard maize. Eur Food Res Technol 214: 449-453.
    • (2002) Eur Food Res Technol , vol.214 , pp. 449-453
    • Holk, A.1    Va-Lucdiderjerjean, M.2    Rudi, K.3
  • 21
    • 3142759654 scopus 로고    scopus 로고
    • The modular analytical procedure and validation approach and the units of measurement for genetically modified materials in foods and feeds
    • Holst-Jensen A, Berdal KG (2004) The modular analytical procedure and validation approach and the units of measurement for genetically modified materials in foods and feeds. J AOAC Int 87: 927-936.
    • (2004) J AOAC Int , vol.87 , pp. 927-936
    • Holst-Jensen, A.1    Berdal, K.G.2
  • 24
    • 8444235413 scopus 로고    scopus 로고
    • Qualitative and quantitative evaluation of the genomic DNA extracted from GMO and non-GMO foodstuffs with four different extraction methods
    • Peano C, Samson MC, Palmieri L, Gulli M, Marmiroli N (2004) Qualitative and quantitative evaluation of the genomic DNA extracted from GMO and non-GMO foodstuffs with four different extraction methods. J Agric Food Chem 52: 6962-6968.
    • (2004) J Agric Food Chem , vol.52 , pp. 6962-6968
    • Peano, C.1    Samson, M.C.2    Palmieri, L.3    Gulli, M.4    Marmiroli, N.5
  • 26
    • 16544384956 scopus 로고    scopus 로고
    • Sigmoidal curve-fitting redefines quantitative Real-Time PCR with the prospective of developing automated high-throughput applications
    • Rutledge RG (2004) Sigmoidal curve-fitting redefines quantitative Real-Time PCR with the prospective of developing automated high-throughput applications. Nucleic Acids Res 32(22): e178.
    • (2004) Nucleic Acids Res , vol.32 , Issue.22
    • Rutledge, R.G.1
  • 27
    • 0036726564 scopus 로고    scopus 로고
    • Validation of Real-Time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules
    • Shindo Y, Kuribara H, Matsuoka T, Futo S, Sawada C, Shono J, Akiyama H, Goda Y, Toyoda M, Hino A (2002) Validation of Real-Time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. J AOAC Int 85: 1119-1126.
    • (2002) J AOAC Int , vol.85 , pp. 1119-1126
    • Shindo, Y.1    Kuribara, H.2    Matsuoka, T.3    Futo, S.4    Sawada, C.5    Shono, J.6    Akiyama, H.7    Goda, Y.8    Toyoda, M.9    Hino, A.10
  • 28
    • 13844250034 scopus 로고    scopus 로고
    • Cloned plasmid DNA fragments as calibrators for controlling GMOs: Different real-time duplex quantitative methods
    • Taverniers I, van Bockstaele E, De Loose M (2004) Cloned plasmid DNA fragments as calibrators for controlling GMOs: Different real-time duplex quantitative methods. Anal Bioanal Chem 378: 1198-1207.
    • (2004) Anal Bioanal Chem , vol.378 , pp. 1198-1207
    • Taverniers, I.1    van Bockstaele, E.2    de Loose, M.3
  • 29
  • 30
    • 0036560919 scopus 로고    scopus 로고
    • Detection of genetically modified crops and their derivatives: critical steps in sample preparation and extraction
    • Terry F, Harris N, Parkes H (2002) Detection of genetically modified crops and their derivatives: critical steps in sample preparation and extraction. J AOAC Int 85: 768-774.
    • (2002) J AOAC Int , vol.85 , pp. 768-774
    • Terry, F.1    Harris, N.2    Parkes, H.3
  • 31
    • 1542356381 scopus 로고    scopus 로고
    • Standardized determination of Real-Time PCR efficiency from a single reaction set-up
    • Tichopad A, Dilger M, Schwarz G, Pfaffl MW (2003) Standardized determination of Real-Time PCR efficiency from a single reaction set-up. Nucleic Acids Res 31(20): e122.
    • (2003) Nucleic Acids Res , vol.31 , Issue.20
    • Tichopad, A.1    Dilger, M.2    Schwarz, G.3    Pfaffl, M.W.4
  • 32
    • 13444291036 scopus 로고    scopus 로고
    • Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction
    • van LT, Paquet N, Calvo E, Cumps J (2005) Improved real-time RT-PCR method for high-throughput measurements using second derivative calculation and double correction. Biotechniques 38: 287-293.
    • (2005) Biotechniques , vol.38 , pp. 287-293
    • van, L.T.1    Paquet, N.2    Calvo, E.3    Cumps, J.4
  • 34
    • 42549086265 scopus 로고    scopus 로고
    • Impact of genetic structures on haploid genome-based quantification of genetically modified DNA: theoretical considerations, experimental data in MON 810 maize kernels (Zea mays L.) and some practical applications
    • Zhang D, Corlet A, Fouilloux S (2008) Impact of genetic structures on haploid genome-based quantification of genetically modified DNA: theoretical considerations, experimental data in MON 810 maize kernels (Zea mays L.) and some practical applications. Transgenic Res 17(3): 393-402.
    • (2008) Transgenic Res , vol.17 , Issue.3 , pp. 393-402
    • Zhang, D.1    Corlet, A.2    Fouilloux, S.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.