Event-specific plasmid standards and real-time PCR methods for transgenic Bt11, Bt176, and GA21 maize and transgenic GT73 canola

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 8, 2005, Pages 3041-3052

  Taverniers, Isabel ^a   Windels, Pieter ^a   Vaïtilingom, Marc ^b   Milcamps, Anne ^c   Van Bockstaele, Erik ^a,d   Van Den Eede, Guy ^c   De Loose, Marc ^a  

^a Centrum voor Landbouwkundig Onderzoek Gent CLO / Centre de Recherches Agronomiques Gand   (Belgium)

^b Ctr des Rech Nucleaires   (France)

^c JOINT RESEARCH CENTRE   (Italy)

^d GHENT UNIVERSITY   (Belgium)

Author keywords

Bt11 maize; Bt176 maize; Event specific marker; GA21 maize; GMO; GT73 canola; PCR identification and quantification

Indexed keywords

DNA; DNA MARKER; GENOMIC DNA; MARKER; PLASMID DNA; THORIUM;

ARTICLE; CALIBRATION; CLONE; DISSOCIATION; DNA SYNTHESIS; GENE LOCUS; GENE SEQUENCE; GENE TARGETING; GENETIC ANALYSIS; GENETIC MARKER; GENETICALLY MODIFIED FOOD; GENOME; MAIZE; MOLECULAR CLONING; MOLECULAR GENETICS; MOLECULAR PROBE; NONHUMAN; PLASMID; PLASMID VECTOR; POLYMERASE CHAIN REACTION; QUALITATIVE ANALYSIS; RAPESEED; REAL TIME POLYMERASE CHAIN REACTION; SAMPLE; SEQUENCE ANALYSIS; STANDARD; TRANSGENE;

BRASSICA NAPUS VAR. NAPUS; SINAPIS ARVENSIS; ZEA MAYS;

EID: 17144398809     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf0483467     Document Type: Article

References (38)

1. Directive 2001/18/EC on the deliberate release into the environment of genetically modified organisms and repealing Council Kirective 90/220/EEC. Off. J. Eur. Union 2001, 106, 1-37, 17.04.2001.
2. Regulation (EC) number 1829/2003 on genetically modified food and feed, Off. J. Eur. Union 2003, 268, 1-23, 18.10.2003.
3. Regulation (EC) number 1830/2003 concerning the traceability and labeling of genetically modified organisms and the traceability of food and feed products produced from GMOs, Off. J. Eur. Union 2003, 268, 24-28, 18.10.2003.
4. Anklam, E.; Gadani, F.; Heinze, P.; Pijnenburg, H.; van den Eede, G. Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived products. Eur. Food Res. Technol 2002, 214, 3-26.
5. Holst-Jensen, A.; Ronning; S. B.; Sovseth, A.; Berdal, K. G. PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal Bioanal. Chem. 2003, 375, 985-993.
6. Berdal, K. G.; Holst-Jensen, A. Roundup Ready soybean event-specific real-time PCR assay and estimation of the practical detection and quantification limits in GMO analyses. Eur. Food Res. Technol. 2001, 213, 432-438.
7. Taverniers, I.; Windeis, P.; van Bockstaele, E.; De Loose, M. Use of cloned DNA fragments for event-specific quantification of genetically modified organisms in pure and mixed food products. Eur. Food Res. Technol. 2001, 213, 417-424.
8. Terry, C. F.; Harris, N. Event-specific detection of Roundup Ready Soya using two different real time PCR detection chemistries. Eur. Food Res. Technol. 2001, 213, 425-431.
9. Burns, M.; Shanahan, D.; Valdivia, H.; Harris, N. Quantitative event-specific multiplex PCR detection of Roundup Ready soya using LabChip technology. Eur. Food Res. Technol. 2003, 216, 428-433.
10. Hernandez, M.; Pla, M.; Esteve, T.; Prat, S.; Puigdomenech, P.; Ferrando, A. A specific real-time quantitative PCR detection system for event MON810 in maize YieldGard based on the 3′-transgene integration sequence. Transgenic Res. 2003, 12, 179-189.
11. Holck, A.; Vaïtilingom, M.; Didierjean, L.; Rudi, K. 5′-Nuclease PCR for quantitative event-specific detection of the genetically modified Mon810 MaisGard maize. Eur. Food Res. Technol. 2002, 214, 449-453.
12. Windeis, P.; Bertrand, S.; Depicker, A.; Moens, W.; van Bockstaele, E.; De Loose, M. Qualitative and event-specific PCR real-time detection methods for StarLink maize. Eur. Food Res. Technol. 2003, 216, 259-263.
13. Zimmermann, A.; Lüthy, J.; Pauli, U. Event specific transgene detection in Bt11 corn by quantitative PCR at the integration site. Lebensm.-Wiss. Technol. 2000, 33, 210-216.
14. Ronning, S. B.; Vaïtilingom, M.; Berdal, K. G.; Holst-Jensen, A. Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays). Eur. Food Res. Technol. 2003, 276, 347-354.
15. Nielsen, C. R.; Berdal K. G.; Holst-Jensen, A. Characterization of the 5′ integration site and development of an event-specific real-time PCR assay for NK603 maize from a low starting copy number. Eur. Food Res. Technol. 2004, 219, 421-427.
16. Hernandez, M.; Esteve, T.; Prat, S.; Pla, M. Development of real-time PCR systems based on SYBR Green I, Amplifluor, and TaqMan technologies for specific quantitative detection of the transgenic maize event GA21. J. Cereal Sci. 2004, 39, 99-107.
17. Holst-Jensen, A.; Berdal, K. G. The modular analytical procedure and validation approach and the units of measurement for genetically modified materials in foods and feeds. J. AOAC Int. 2004, 87, 1-9.
18. Commission Recommendation 2004/787/EC of 4 October 2004 on technical guidance for sampling and detection of genetically modified organisms and material produced from genetically modified organisms as or in products in the context of Regulation (EC) number 1830/2003. Off. J. Eur. Union 2004, 348, 18-26.
19. Block, A.; Schwarz, G. Validation of different genomic and cloned DNA calibration standards for construct-specific quantification of Liberty Link in rapeseed by real-time PCR. Eur. Food Res. Technol. 2003, 216, 421-427.
20. Kuribara, H.; Shindo, Y.; Matsuoka, T.; Takubo, K.; Futo, S.; Aoki, N.; Hirao, T.; Akiyama, H.; Goda, Y.; Toyoda, M.; Hino, A. Novel reference molecules for quantitation of genetically modified maize and soybean. J. AOAC Int. 2002, 85, 1077-1089.
21. Shindo, Y.; Kuribara, H.; Matsuoka, T.; Futo, S.; Sawada, C.; Shono, J.; Akiyama, H.; Goda, Y.; Toyoda, M.; Hino, A. Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. J. AOAC Int. 2002, 85, 1119-1126.
22. Taverniers, I.; van Bockstaele, E.; De Loose, M. Cloned plasmid DNA fragments as calibrators for controlling GMOs: Different real-time duplex quantitative methods. Anal. Bioanal. Chem. 2004, 378, 1198-1207.
23. Terry, C. F.; Shanahan, D. J.; Ballam, L. D.; Harris, N.; McDowell, D. G.; Parkes, H. C. Real-time detection of genetically modified soya using LightCycler and ABI7700 platforms with TaqMan, Scorpion, and SYBR Green I chemistries. J. AOAC Int. 2002, 85, 938-944.
24. Council Directive 90/220/EEC of 23 April 1990 on the deliberate release into the environment of genetically modified organisms. Off. J. Eur. Commun. 1990, 117, 15-27.
25. Regulation (EC) number 258/97 of the European Parliament and of the Council of 27 January 1997 concerning novel foods and novel food ingredients. Off. J. Eur. Commun. 1997, 43, 1-6.
26. Arumuganathan, K.; Earle, E. D. Nuclear DNA content of some important plant species. Plant Mol. Biol. Rep. 1991, 9, 208-218.
27. Windels, P.; Taverniers, I.; Depicker, A.; van Bockstaele, E.; De Loose, M. Characterization of the Roundup Ready soybean insert. Eur. Food Res. Technol. 2001, 213, 107-112.
28. Theuns, I.; Windels, P.; De Buck, S.; Depicker, A.; van Bockstaele, E.; De Loose, M. Identification and characterization of T-DNA inserts by T-DNA fingerprinting. Euphytica 2002, 123, 75-84.
29. Rasmussen, R. Quantification on the LightCycler Instrument. In Rapid Cycle Real-Time PCR, Methods, and Applications; Meuer, S.; Wittwer, C.; Nakagara, K., Eds.; Springer Press: Heidelberg, Germany, 2001; pp 21-34.
30. Summary Notification Information Format (SNIP) for products containing genetically modified higher plants (GMHPs) - Roundup Ready oilseed rape (Brassica napus) derived from event GT73. http://www.biosafety.be/TP/SNIFs/C-NL- 98-11.pdf, 20 pages.
31. Hernandez, M.; Rodriguez-Lazaro, D.; Esteve, T.; Prat, S.; Pla, M. Development of melting temperature-based SYBR Green I polymerase chain reaction methods for multiplex genetically modified organism detection. Anal. Biochem. 2003, 323, 164-170.
32. Ririe, K. M.; Rasmussen, R. P.; Wittwer, C. T. Product differentiation by analysis of DNA melting curves during the polymerase chain reaction. Anal. Biochem. 1997, 245, 154-160.
33. Li, W.; Xi, B.; Yang, W.; Hawkins, M.; Schubart, U. K. Complex DNA melting profiles of small PCR products revealed using SYBR Green I. BioTechniques 2003, 34, 702-706.
34. Shepherd, M.; Henry, R. Monitoring of fluorescence during DNA melting as a method for discrimination and detection of PCR products in variety identification. Mol. Breed. 1998, 4, 509-517.
35. Birch, L.; Archard, C. L.; Parkes, H. C.; McDowell, D. G. Evaluation of LabChipTM technology for GMO analysis in food. Food Contr. 2001, 12, 535-540.
36. Garcia-Canas, V.; Gonzales, R.; Cifuentes, A. Detection of genetically modified maize by the polymerase chain reaction and capillary gel electrophoresis with UV detection and laser induced fluorescence. J. Agric. Food Chem. 2002, 50, 1016-1021.
37. Garcia-Canas, V.; Gonzales, R.; Cifuentes, A. Ultrasensitive detection of genetically modified maize by capillary electrophoresis with laser induced fluorescence using different fluorescent intercalating dyes. J. Agric. Food Chem. 2002, 50, 4497-4502.
38. ISO Guide 17511. In Vitro Diagnostic Medical Devices - Measurement of Quantities in Biological Samples - Metrological Traceability of Values Assigned to Calibrators and Control Materials; ISO: Geneva, Switzerland, 2003.