Production of functional recombinant bovine trypsin in transgenic rice cell suspension cultures

Protein Expression and Purification

Volumn 76, Issue 1, 2011, Pages 121-126

  Kim, Nan Sun ^a   Yu, Hwa Young ^a   Chung, Nguyen Duc ^a   Shin, Yun Ji ^b   Kwon, Tae Ho ^b   Yang, Moon Sik ^a  

^a Chonbuk National University   (South Korea)

^b Jeonju Bio Materials Institute   (South Korea)

Author keywords

Agrobacterium tumefaciens; Bovine trypsin; Codon optimization; Rice amylase 3D promoter; Rice suspension culture

Indexed keywords

RECOMBINANT PROTEIN; TRYPSIN; TRYPSINOGEN;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; BIOSYNTHESIS; CATTLE; CELL CULTURE; CULTURE MEDIUM; GENE VECTOR; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; RICE; TRANSGENIC PLANT;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CATTLE; CELLS, CULTURED; CULTURE MEDIA, CONDITIONED; GENETIC VECTORS; MOLECULAR SEQUENCE DATA; ORYZA SATIVA; PLANTS, GENETICALLY MODIFIED; RECOMBINANT PROTEINS; TRYPSIN; TRYPSINOGEN;

AGROBACTERIUM TUMEFACIENS; BOVINAE; ORYZA SATIVA;

EID: 78650175360     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.10.007     Document Type: Article

References (44)

1. R.M. Stroud, A.A. Kossiakoff, and J.L. Chambers Mechanisms of zymogen activation Annu. Rev. Biophys. Bioeng. 6 1997 177 193
2. H. Huber, and W. Bode Structural basis of the activation and action of trypsin Acc. Chem. Res. 11 1978 114 122
3. H. Neurath, and K.A. Walsh Role of proteolytic enzymes in biological regulation Proc. Natl. Acad. Sci. USA 73 1976 3825 3832
4. J. Leipner, and R. Saller Systemic enzyme therapy in oncology: effect and mode of action Drugs 59 2000 769 780 (Pubitemid 30232319)
5. W. Kemmler, J.D. Peterson, and D.F. Steiner Studies on the conversion of proinsulin to insulin. I. Conversion in vitro with trypsin and carboxypeptidase B J. Biol. Chem. 246 1971 6786 6791
6. R. Chance, R.D. Marchi, B. Frank, J.E. Shields, Process for preparing insulin analogs, S. Patent 5, 700,662., 1997.
7. B. Frank, W. Prouty, M. Walden, Process for transforming a human insulin precursor to human insulin. US patent 5457,066., 1995.
8. C.S. Craik, R. Rocaniak, C. Largman, and W.J. Rutter The catalytic role of the active site aspartic acid in serine proteases Science 237 1987 909 913 (Pubitemid 17128282)
9. C.S. Craik, C. Largman, T. Fletcher, S. Roczniak, P.J. Barr, R.J. Fletterick, and W.J. Rutter Redesigning trypsin: alteration of substrate specificity, catalytic activity and protein conformation Science 228 1985 291 297 (Pubitemid 15076825)
10. C.N.M. Nyaruhucha, M. Kito, and S.L. Fukuoka Identification and expression of the cDNA-encoding human mesotrypsin (ogen). An isoform of trypsin with inhibitor resistance J. Biol. Chem. 272 1997 10573 10578 (Pubitemid 27181064)
11. J.R. Vasquez, L.B. Evnin, J.N. Higaki, and C.S. Craik An expression system for trypsin J. Cell. Biochem. 39 1989 265 276 (Pubitemid 19078632)
12. L.B. Evine, and C.S. Craik Development of an efficient method for generating and screening active trypsin and trypsin variants Ann. NY Acad. Sci. 542 1988 61 74 (Pubitemid 19115084)
13. J. Hanquier, Y. Sorlet, D. Desplancq, L. Baroche, M. Ebtinger, J.F. Lefevre, F. Pattus, C.L. Hershberger, and A.A. Vertes A single mutation in the activation site of bovine trypsinogen enhances its accumulation in the fermentation broth of the yeast Picha pastoris Appl. Environ. Microbial. 69 2003 1108 1113 (Pubitemid 36204841)
14. A.L. Niles, M. Maffitt, M. Haak-Frendscho, C.J. Wheeless, and D.A. Johnson Recombinant human mast cell tryptase beta: stable expression in Pichia pastoris and purification of fully active enzyme Biotechnol. Appl. Biochem. 28 1998 125 131 (Pubitemid 28470097)
15. J.L. Harris, E.P. Peterson, D. Hudig, N.A. Thomberry, and C.S. Craik Definition and redesign of the extended substrate specificity of granzyme B J. Biol. Chem. 273 1998 27364 27373 (Pubitemid 28500453)
16. R.M. Twyman, E. Stoger, S. Schillberg, P. Christou, and R. Fischer Molecular farming in plants: host systems and expression technology Trends Biotechnol. 21 2003 570 578 (Pubitemid 37415010)
17. J.K. Ma, P.M. Drake, and P. Christou The production of recombinant pharmaceutical proteins in plants Nat. Rev. Genetics 4 2003 794 805 (Pubitemid 37193271)
18. E.E. Hood, S.L. Woodard, and M.E. Hom Monoclonal antibody manufacturing in transgenic plants-myths and realities Curr. Opin. Biotechnol. 13 2002 630 635 (Pubitemid 35448068)
19. A.R. Kusnadi, E.E. Hood, D.R. Witcher, J.A. Howard, and Z.L. Nikolove Production and purification of two recombinant proteins from transgenic corn Biotechnol. Prog. 14 1998 149 155 (Pubitemid 28118276)
20. S.L. Woodard, J.M. Mayor, M.R. Bailey, D.K. Barker, R.T. Love, J.R. Lane, D.D. Delaney, J.M. McComas-Wagner, H.D. Mallubhotla, E.E. Hood, L.J. Dangott, S.E. Tichy, and J.A. Howard Maize-derived bovine trypsin: characterization of the first large-scale, commercial protein product from transgenic plants Biotechnol. Appl. Biochem. 38 2003 123 130 (Pubitemid 37289826)
21. R.L. Evangelista, A.R. Kusnadi, J.A. Howard, and Z.L. Nikolov Process and economic evaluation of the extraction and purification of recombinant glucuronidase from transgenic corn Biotechnol. Prog. 14 1998 607 614 (Pubitemid 28382654)
22. A.R. Kusnadi, Z.L. Nikolov, and J.A. Howard Production of recombinant proteins in transgenic plants: practical consideration Biotechnol. Bioeng. 56 1997 473 484 (Pubitemid 27471425)
23. C. Plasson, R. Michel, D. Lienard, C. Saint-Jore-Dupas, C. Sourrouille, G.G. de March, and V. Gomord Production of recombinant proteins in suspension-cultured plant cells Methods Mol. Biol. 483 2009 145 161
24. P. Hajdukiewicz, Z. Svab, and P. Maliga The small versatile pPZP family of Agrobacterium binary vectors for plant transformation Plant Mol. Biol. 25 1994 989 994 (Pubitemid 2152867)
25. A. Hoekema, P.R. Hirsch, P.J.J. Hooykaas, and R.A. Schiperoort A binary plant vector strategy based on separation of vir and T-region of Agrobacterium tumefaciens Ti-plasmid Nature 303 1983 179 180 (Pubitemid 13081082)
26. E. Van Haute, H. Joos, M. Maes, G. Warren, M. Van Montagu, and J. Schell Intergeneric transfer and exchange recombination of restriction fragments cloned in pBR322: a novel strategy for the reversed genetics of the Ti plasmids of Agrobacterium tumefaciens EMBO J. 2 1983 411 417
27. Y. Hiei, S. Ohta, T. Komari, and T. Kumashiro Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA Plant J. 6 1994 271 282
28. J.A. Thompson, R. Abdullah, and E.C. Cocking Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose Plant Sci. 47 1986 123 133
29. J. Sambrook, and D.W. Russell Molecular Cloning: A Laboratory Manual 3rd ed 2001 Cold Spring Harbor Laboratory Press Cold Spring Harbor.
30. U.K. Laemmli Cleavage of structural proteins during the assembly of the head bacteriophage T4 Nature 227 1970 680 685
31. C. Heussen, and C.B. Dowdle Electrophoretic analysis of plasminogen activators in polyacrylamide gels containing sodium dodecyl sulfate and copolymerized substrates Anal. Biochem. 102 1980 196 202 (Pubitemid 10099499)
32. M.H. Chen, L.F. Liu, Y.R. Chen, H.K. Wu, and S.M. Yu Expression of alpha-amylase, carbohydrate metabolism, and autophagy in cultured rice cells is coordinately regulated by sugar nutrient Plant J. 6 1994 625 636
33. S. Harcum, and W. Bentley Detection, quantification, and characterization of protease in recombinant Escherichia coli Biotechnol. Technol. 7 1993 441 447
34. K.M. Hummel, A.R. Penheiter, A.C. Gathman, and W.W. Lilly Anomalous estimation of protease molecular weights using gelatin-containing SDS-PAGE Anal. Biochem. 233 1996 140 142 (Pubitemid 126335202)
35. D.R. Corey, A.K. Shiau, Q. Yang, B.A. Janowski, and C.S. Craik Trypsin display on the surface of bacteriophage Gene 128 1993 129 134 (Pubitemid 23179220)
36. S. Hellwig, J. Drossard, R.M. Twyman, and R. Fischer Plant cell culture for the production of recombinant proteins Nat. Biotechnol. 22 2004 1415 1422 (Pubitemid 39482861)
37. N. Huang, T.D. Sutliff, J.C. Litts, and R.L. Rodriguez Classification and characterization of the rice α-amylase multigene family Plant Mol. Biol. 14 1990 655 688
38. Y.J. Shin, S.Y. Shin, T.H. Kwon, Y.S. Jang, and M.S. Yang High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture Biotechnol. Bioeng. 82 2003 778 783
39. T.G. Kim, M.Y. Baek, E.K. Lee, T.H. Kwon, and M.S. Yang Expression of human growth hormone in transgenic rice cell suspension culture Plant Cell Rep. 27 2008 885 891
40. S.Y. Hong, T.H. Kwon, Y.S. Jang, S.H. Kim, and M.S. Yang Production of bioactive human granulocyte-colony stimulating factor in transgenic rice cell suspension culture Protein Expr. Purif. 47 2006 68 73
41. L.F. Huang, Y.K. Liu, C.A. Lu, S.L. Hsieh, and S.M. Yu Production of human serum albumin by sugar starvation induced promoter and rice cell culture Transgenic Res. 14 2005 569 581
42. M. Terashima, Y. Muri, M. Kawamura, S. Nkanishi, T. Stolz, L. Chen, W. Drohan, R.L. Rodriguez, and S. Katoh Production of functional human 1-antitrypsin by plant cell culture Appl. Microbiol. Biotechnol. 52 1992 516 523
43. L.G. Jensen, O. Olsen, O. Kops, N. Wolf, and K.K. Thomsen Transgenic barley expressing a protein-engineered, thermostable (1, 3-1, 4)-β-glucanase during germination Proc. Natl. Acad. Sci. USA 93 1996 3487 3491
44. C.H. Kim, Y. Oh, and T.H. Lee Codon optimization for high-level expression of human erythropoietin (EPO) in mammalian cells Gene 199 1997 293 301 (Pubitemid 27448231)