High level of expression of recombinant human granulocyte-macrophage colony stimulating factor in transgenic rice cell suspension culture

Biotechnology and Bioengineering

Volumn 82, Issue 7, 2003, Pages 778-783

  Shin, Yun Ji ^a   Hong, Shin Young ^a   Kwon, Tae Ho ^a   Jang, Yong Suk ^a   Yang, Moon Sik ^a  

^a Chonbuk National University   (South Korea)

Author keywords

Ramy3D; Recombinant human GM CSF; Transgenic rice cell suspension culture

Indexed keywords

GENES; PROTEINS; TOBACCO;

HETEROLOGOUS GENE EXPRESSION;

BIOTECHNOLOGY;

AMYLASE; PROTEINASE; RECOMBINANT GRANULOCYTE MACROPHAGE COLONY STIMULATING FACTOR;

ARTICLE; CULTURE MEDIUM; ENZYME ACTIVITY; EXPRESSION VECTOR; GENE; GENE EXPRESSION; GENE EXPRESSION SYSTEM; NONHUMAN; NORTHERN BLOTTING; PROMOTER REGION; QUANTITATIVE ASSAY; RAMY3D GENE; RICE; SUSPENSION CELL CULTURE; TOBACCO; TRANSGENIC PLANT; TRANSGENICS; WESTERN BLOTTING;

CELLS, CULTURED; ENDOPEPTIDASES; GENE EXPRESSION REGULATION, PLANT; GRANULOCYTE MACROPHAGE COLONY-STIMULATING FACTORS, RECOMBINANT; HUMANS; ORYZA SATIVA; PLANTS, GENETICALLY MODIFIED; PROTEIN ENGINEERING; QUALITY CONTROL; RECOMBINANT PROTEINS; TOBACCO;

NICOTIANA TABACUM;

EID: 0037572329     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.10635     Document Type: Article

References (23)

1. Battaglino R, Huergo M, Pilosof A, Bartholoma, G. 1991. Culture requirement for the production of protease by Aspergillus oryzae in solid state fermentation. Appl Microb Biotechnol 35:292.
2. Chen LL, Marmey P, Taylor NJ, Brizard JP, Espinoza C, Dcruz P, Huet H, Zhang SP, deKochko A, Beachy RN, Fauquet CM. 1998. Expression and inheritance of multiple transgenes in rice plants. Nat Biotechnol 16:1060-1064.
3. Chu CC, Wang CC, Sun CS, Hsu C, Yin KC, Chu CY, Bi FY. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Sci Sin 18:659-668.
4. Daniell H, Streat SJ, Wycoff K. 2001. Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci 6:219-226.
5. Doran P. 2000. Foreign protein production in plant tissue cultures. Curr Opin Biotechnol 11:199-204.
6. Huang J, Sutliff T, Wu L, Nandi L, Somen K, Benge A, Kelli M, Terashima M, Ralton A, Drohan W, Huang N, Rodriguez R. 2001. Expression and purification of functional recombinant human α-1antitrypsin from cultured plant cells. Biotechnol Prog 17:126-133.
7. Huang J, Wu L, Yalda D, Adkins Y, Kelleher S, Crane M, Lonnerdal B, Rodriguez R, Huang N. 2002. Expression of functional recombinant human lysozyme in transgenic rice cell culture. Transgenic Res 11:229-239.
8. Huang N, Chandler J, Thomas B, Koizumi N, Rodriguez R. 1993. Metabolic regulation of amylase gene expression in transgenic cell cultures of rice (Oryza sativa L.). Plant Mol Biol 23:737-747.
9. James E, Wang C, Wang Z, Reeves R, Shin J, Magnuson N, Lee J. 2000. Production and characterization of biologically active human GM-CSF secreted by genetically modified plant cells. Protein Expr Purif 19:131-138.
10. Kitamura T, Tange T, Terasawa T, Chiba S, Kuwaki T, Miyagawa K, Piao YF, Miyazono K, Urabe A, Takaku F. 1989. Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J Cell Physiol 140:323-334.
11. LaCount W, An GH, Lee JM. 1997. The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody production from plant suspension cultures. Biotechnol Lett 19:93-96.
12. Lee F, Yokota T, Otsuka T, Gemmell L, Larson N, Luh J, Arai K, Rennick D. 1985. Isolation of cDNA for a human granulocyte-macrophage colony stimulation factor by functional expression in mammalian cells. Proc Natl Acad Sci USA 82:4360-4364.
13. Lee J, Choi S, Kang H, Oh W, Cho K, Kwon T, Kim D, Jang Y, Yang M. 1997. Establishment of a transgenic tobacco cell suspension culture system for producing murine granulocyte-macrophage colony stimulating factor. Mol Cells 7:783-787.
14. Lee J, Kim N, Kwon T, Jang Y, Yang M. 2002. Increased production of human granulocyte-macrophage colony stimulating factor (hGM-CSF) by the addition of stabilizing polymer in plant suspension cultures. J Biotechnol 96:205-211.
15. Rasko JE, Gough NM. 1994. Granulocyte-macrophage colony stimulating factor. In: Thomson A, editor. The cytokine handbook. London: Academic Press. p 343-369.
16. Sambrook J, Fritsch EF, Maniatis T. 1989. Molecular cloning. Plainview, NY: Cold Spring Harbor Laboratory Press.
17. Sharp J, Doran P. 1999. Effect of bacitracin on growth and monoclonal antibody production by tobacco hairy roots and cell suspensions. Biotechnol Bioproc Eng 4:253-258.
18. Terashima M, Ejiri Y, Hashikawa N, Yoshida H. 1999. Effect of osmotic pressure on human antitrypsin production by plant cell culture. Biochem Eng J 4:31-36.
19. Terashima M, Murai Y, Kawamura M, Nakanishi S, Stoltz T, Chen L, Drohan W, Rodriguez R, Katoh S. 1999. Production of functional human α-1-antitrypsin in plant cell culture. Appl Microb Biotechnol 52:516-519.
20. Thompson JA, Abdullah R, Cocking EC. 1986. Protoplast culture of rice (Oryza sativa L.) using media solidified with agarose. Plant Sci 47:123-133.
21. Towbin H, Staehelin T, Gordon J. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Biotechnology 24:145-149.
22. Toyofuku K, Umemura T, Yamaguchi J. 1998. Promoter elements required for sugar-repression of the Ramy3D gene for α-amylase in rice. FEBS Lett 428:275-280.
23. Wongsamuth R, Doran P. 1997. Production of monoclonal antibodies by tobacco hairy roots. Biotechnol Bioeng 54:401-415.