Recombinant human mast cell tryptase: Stable expression in Pichia pastoris and purification of fully active enzyme

Biotechnology and Applied Biochemistry

Volumn 28, Issue 2, 1998, Pages 125-131

  Niles, Andrew L ^a   Maffitt, Mark ^a   Haak Frendscho, Mary ^a   Wheeless, Christine J ^b   Johnson, David A ^b  

^a PROMEGA CORPORATION   (United States)

^b East Tennessee State University   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; ASPARAGINE; METHANOL; RECOMBINANT ENZYME; SERINE PROTEINASE; TRYPTASE;

AFFINITY CHROMATOGRAPHY; ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; ENDOMYCETALES; ENZYME GLYCOSYLATION; ENZYME KINETICS; ENZYME PURIFICATION; EXPRESSION VECTOR; HUMAN; HUMAN CELL; MAST CELL; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS;

CHROMATOGRAPHY, GEL; CHYMASES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESTERS; GLYCOSIDE HYDROLASES; GLYCOSYLATION; HUMANS; ISOENZYMES; KINETICS; MAST CELLS; PICHIA; RECOMBINANT PROTEINS; SERINE ENDOPEPTIDASES; SKIN; TRYPTASES;

FELIS CATUS; PICHIA; PICHIA PASTORIS; SACCHAROMYCETALES;

EID: 0031657134     PISSN: 08854513     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Glenner, G. G. and Cohen, L. A. (1960) Nature (London) 185, 846-847
2. Alter, S. C., Metcalfe, D. D., Bradford, T. R. and Schwartz, L. B. (1987) Biochem. J. 248, 821-827
3. Schwartz, L. B., Lewis, R. A. and Austen, K. F. (1981) J. Biol. Chem. 256, 11939-11943
4. Smith, T. J., Hougland, M. W. and Johnson, D. A. (1984) J. Biol. Chem. 259, 11046-11051
5. Harvima, I. T., Schlechter, N. M., Harvima, R. J. and Fraki, J. E. (1988) Biochim. Biophys. Acta 957, 71-80
6. Cromlish, J. A., Seidah, N. G., Marcinkiewicz, M., Hamelin, J., Johnson, D. A. and Chretien, M. (1987) J. Biol. Chem. 262, 1363-1373
7. Miller, J. S., Westin, E. H. and Schwartz, L. B. (1989) J. Clin. Invest. 84, 1188-1195
8. Miller, J. S., Moxley, G. and Schwartz, L. B. (1990) J. Clin. Invest. 86, 864-870
9. Butterfield, J. H., Weiler, D. A., Hunt, L. W., Wynn, S. R. and Roche, P. C. (1990) J. Leukoc. Biol. 47, 409-419
10. Vanderslice, P., Ballinger, S. M., Tam, E. K., Goldstein, S. M., Craik, C. S. and Caughey, G. H. (1990) Proc. Natl. Acad. Sci. U.S.A. 87, 3811-3815
11. Sakai, K., Ren, S. and Schwartz, L. B. (1996) J. Clin. Invest. 97, 988-995
12. Walls, A. F., Bennett, A. R., McBride, H. M., Glennie, M. J., Holgate, S. T. and Church, M. K. (1990) Clin. Exp. Allergy 20, 581-589
13. InVitrogen Corporation (1996) A Manual of Methods for Expression of Recombinant Proteins in Pichia pastoris, Version F, catalogue no. K170-01, p. 59, InVitrogen, San Diego
14. Wung, J. L. and Gascoigne, N. R. (1996) Biotechniques 21, 808
15. Erlanger, B. J., Kokowsky, N. and Cohen, W. (1961) Arch. Biochem. Biophys. 95, 271-278
16. Addington, A. K. and Johnson, D. A. (1996) Biochemistry 35, 13511-13518
17. Jameson, G. W., Roberts, D. V., Adams, R. W., Kyle, W. S. A. and Elmore, D. T. (1973) Biochem. J. 131, 107-117
18. Laemmli, U.K. (1970) Nature (London) 227, 680-685
19. Smith, T. J. and Johnson, D. A. (1984) Fed. Proc. Fed. Am. Soc. Exp. Biol. 43, 1760
20. Schwartz, L. B. and Bradford, T. R. (1986) J. Biol. Chem. 261, 7372-7379
21. Schechter, N. M., Eng, G. Y. and McCaslin, D. R. (1993) Biochemistry 32, 2617-2625
22. Schechter, N. M., Eng, G. Y., Selwood, T. and McCaslin, D. R. (1995) Biochemistry 34, 10628-10638
23. Fiorucci, L., Erba, F. and Ascoli, F. (1992) Biol. Chem. Hoppe-Seyler 373, 483-490
24. Thomas, V. A., Wheeless, C. J., Stack, M. S. and Johnson, D. A. (1998) Biochemistry, in the press
25. He, S., Peng, Q. and Walls, A. F. (1997) J. Immunol. 159, 6216-6225