N-acetylcysteine counteracts oxidative stress and prevents hCG-induced apoptosis in rat Leydig cells through down regulation of caspase-8 and JNK

Molecular Reproduction and Development

Volumn 77, Issue 10, 2010, Pages 900-909

  Aggarwal, Archana ^a   Misro, Man Mohan ^a   Maheshwari, Ankur ^a,b   Sehgal, Neeta ^c   Nandan, Deoki ^a  

^a NATIONAL INSTITUTE OF HEALTH AND FAMILY WELFARE   (India)

^b KURUKSHETRA UNIVERSITY   (India)

^c UNIVERSITY OF DELHI   (India)

Author keywords

Extrinsic pathway; HCG induced apoptosis; Leydig cell; NAC; Oxidative stress

Indexed keywords

ACETYLCYSTEINE; CASPASE 8; CATALASE; CHORIONIC GONADOTROPIN; GLUTATHIONE; GLUTATHIONE TRANSFERASE; REACTIVE OXYGEN METABOLITE; STRESS ACTIVATED PROTEIN KINASE; SUPEROXIDE DISMUTASE;

ANIMAL CELL; ANTIOXIDANT ACTIVITY; APOPTOSIS; ARTICLE; CELL VIABILITY; CONTROLLED STUDY; DOWN REGULATION; DRUG EFFECT; ENZYME ACTIVATION; ENZYME ACTIVITY; GENE EXPRESSION REGULATION; LEYDIG CELL; LIPID PEROXIDATION; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL; RAT;

ACETYLCYSTEINE; ANALYSIS OF VARIANCE; ANIMALS; APOPTOSIS; CASPASE 3; CASPASE 8; CATALASE; CELL SURVIVAL; CHORIONIC GONADOTROPIN; DOWN-REGULATION; GENE EXPRESSION; GLUTATHIONE TRANSFERASE; IN SITU NICK-END LABELING; JNK MITOGEN-ACTIVATED PROTEIN KINASES; LEYDIG CELLS; LIPID PEROXIDATION; MALE; OXIDATIVE STRESS; POLY(ADP-RIBOSE) POLYMERASES; RATS; RATS, SPRAGUE-DAWLEY; REACTIVE OXYGEN SPECIES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SIGNAL TRANSDUCTION; SUPEROXIDE DISMUTASE;

METAZOA; RATTUS;

EID: 78649702197     PISSN: 1040452X     EISSN: 10982795     Source Type: Journal    
DOI: 10.1002/mrd.21232     Document Type: Article

References (44)

1. Aebi H. 1984. Catalse in vitro. Methods Enzymol 105: 121-126.
2. Agarwal A, Said TM. 2005. Oxidative stress, DNA damage and apoptosis in male infertility: A clinical approach. Br J Urol Int 95: 503-507.
3. Aggarwal A, Misro MM, Maheshwari A, Sehgal N, Nandan D. 2009. Adverse effects associated with persistent stimulation of Leydig cells with hCG in vitro. Mol Reprod Dev 76: 1076-1083.
4. Allamaneni SSR, Agarwal A, Nallela KP, Sharma RK, Thomas AJ, Jr., Sikka SC. 2005. Characterization of oxidative stress status by evaluation of reactive oxygen species levels in whole semen and isolated spermatozoa. Fertil Steril 83: 800-803.
5. Anakwe OO, Moger WH. 1986. Catecholamine stimulation of androgen production by rat Leydig cells, interactions with luteinizing hormone and luteinizing hormone-releasing hormone. Biol Reprod 35: 806-814.
6. Bartsch H, Nair J. 2000. Ultrasensitive and specific detection methods for exocytic DNA adducts: Markers for lipid peroxidation and oxidative stress. Toxicology 153: 105-114.
7. Bozec A, Chuzel F, Chater S, Paulin C, Bars R, Benahmed M, Mauduit C. 2004. The mitochondrial-dependent pathway is chronically affected in testicular germ cell death in adult rats exposed in utero to anti-androgens. J Endocrinol 183: 79-90.
8. Briehl MM, Baker AF. 1996. Modulation of the antioxidant defence as a factor in apoptosis. Cell Death Differ 3: 63-70.
9. Carmody RJ, Cotter TG. 2001. Signalling apoptosis: A radical approach. Redox Rep 6: 77-90.
10. Chen H, Liu J, Luo L, Baig MU, Kim JM, Zirkin BR. 2005. Vitamin E, aging and Leydig cell steroidogenesis. Exp Gerontol 40: 728-736.
11. Das K, Samanta L, Chainy GBN. 2000. A modified spectrophotometric assay of superoxide dismutase using nitrite formation of superoxide radicals. Indian J Biochem Biophys 237: 201-204.
12. Erkkila K, Hirvonen V, Wuokko E, Parvinen M, Dunkel L. 1998. N-acetyl-L-cysteine inhibits apoptosis in human male germ cells in vitro. J Clin Endocrinol Metab 83: 2523-2531.
13. Estany S, Palacio JR, Barnadas R, Sabes M, Iborra A, Martinez P. 2007. Antioxidant activity of N-acetylcysteine, flavonoids and α-tocopherol on endometrial cells in culture. J Reprod Immunol 75: 1-10.
14. Farombi EO, Ugwuezunmba MC, Ezenwadu TT, Oyeyemi MO, Ekor M. 2008. Tetracycline-induced reproductive toxicity in male rats: Effects of vitamin C and N-acetylcysteine. Exp Toxicol Pathol 60: 77-85.
15. Flora SD, Izzotti A, D'Agostini F, Balansky RM. 2004. Mechanisms of N-acetylcysteine in the prevention of DNA damage and cancer, with special reference to smoking-related end-points. Carcinogenesis 22: 999-1013.
16. Fujisawa M, Kanzaki M, Tatsumi N, Okuda Y, Okada H, Arakawa S, Kamidono S. 2000. Inhibition of apoptosis in cultured immature rat Leydig cells by human chorionic gonadotropin associated with Bcl-2 mRNA expression. Endocr Res 26: 59-70.
17. 2 levels and induces germ cell apoptosis in the rat testis. Apoptosis 12: 1173-1182.
18. Habig WH, Pabst MJ, Jakoby WB. 1974. Glutathione-s-transferase. J Biol Chem 249: 7130-7139.
19. Hanukoglu I. 2006. Antioxidant protective mechanisms against reactive oxygen species (ROS) generated by mitochondrial P450 systems in steroidogenic cells. Drug Metab Rev 38: 171-196.
20. Havre P, O'Reilly S, McCormick JJ, Brash DE. 2002. Transformed and tumor-derived human cells exhibit preferential sensitivity to the thiol antioxidants, N-acetyl cysteine and penicillamine. Cancer Res 62: 1443-1449.
21. Hockenbery DM, Oltvai ZN, Yin XM, Milliman CL, Korsmeyer SJ. 1993. Bcl-2 functions in an antioxidant pathway to prevent apoptosis. Cell 75: 241-251.
22. 2 in vitro. FEBS J 276: 870-881.
23. Martin H, Abadie C, Heyd B, Mantion G, Richert L, Berthelot A. 2006. N-Acetylcysteine partially reverses oxidative stress and apoptosis exacerbated by Mg-deficiency culturing conditions in primary cultures of rat and human hepatocytes. J Am Coll Nutr 25: 363-369.
24. Mehta A, Sekhon CPS, Giri S, Orak JK, Singh AK. 2002. Attenuation of ischemia/reperfusion induced MAP kinase by N-acetyl cysteine, sodium nitroprusside and phosphoramidon. Mol Cell Biochem 240: 19-29.
25. Nicholson KM, Anderson NG. 2002. The protein kinase B/Akt signalling pathway in human malignancy. Cell Signal 14: 381-395.
26. Oeda T, Henkel R, Ohmori H, Schill WB. 1997. Scavenging effect of N-acetyl-L-cysteine against reactive oxygen species in human semen: A possible therapeutic modality for male factor infertility. Andrologia 29: 125-131.
27. Ohkawa H, Ohishi N, Yagi K. 1979. Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction. Anal Biochem 9: 351-358.
28. Pant N, Srivastava SP. 2003. Testicular and spermatotoxic effect of quinaphos in rats. J Appl Toxicol 23: 271-274.
29. Park SA, Choi KS, Bang JH, Huh K, Kim SU. 2000. Cisplatin-induced apoptotic cell death in mouse hybrid neurons is blocked by antioxidants through suppression of cisplatin-mediated accumulation of p53 but not of Fas/Fas ligand. J Neurochem 75: 946-953.
30. Payabvash S, Salmasi AH, Kiumehr S, Tavangar SM, Nourbakhsh B, Faghihi SH, Dehpour AR. 2007. Salutary effects of N-acetylcysteine on apoptotic damage in a rat model of testicular torsion. Urol Int 79: 248-254.
31. Peltola V, Huhtaniemi I, Ahutopa M. 1992. Antioxidant enzyme activity in the maturing rat testis. J Androl 13: 450-455.
32. Rao AVSK, Shaha C. 2002. N-acetylcysteine prevents MAA induced male germ cell apoptosis: Role of glutathione and cytochrome c. FEBS Lett 527: 133-137.
33. Reid AJ, Shawcross SG, Hamilton AE, Wiberg M, Terenghi G. 2009. N-Acetylcysteine alters apoptotic gene expression in axotomized primary sensory afferent sub-populations. Neurosci Res 65: 148-155.
34. Ruwanpura SM, McLachlan RI, Stanton PG, Loveland KL, Meachem SJ. 2008. Pathways involved in testicular germ cell apoptosis in immature rats after FSH suppression. J Endocrinol 197: 35-43.
35. Shen H-M, Liu Z-G. 2006. JNK signalling pathway is a key modulator in cell death mediated by reactive oxygen and nitrogen species. Free Radic Biol Med 40: 928-939.
36. Shrilata B, Muralidhara. 2007. Early oxidative stress in testis and epididymal sperm in streptozotocin-induced diabetic mice: Its progression and genotoxic consequences. Rep Toxicol 23: 578-587.
37. Slater AF, Stefan C, Nobel I, van den Dobbelsteen DJ, Orrenius S. 1996. Intracellular redox changes during apoptosis. Cell Death Differ 3: 57-62.
38. Tai P, Shiraishi K, Ascoli M. 2009. Activation of the lutropin/choriogonadotropin receptor inhibits apoptosis of immature Leydig cells in primary culture. Endocrinology 150: 3766-3773.
39. L and IAP, the release of cytochrome c and inhibition of Akt. Carcinogenes 24: 1199-1208.
40. Yamamoto K, Ichijo H, Korsmeyer SJ. 1999. BCL-2 is phosphorylated and inactivated by an ASK1/Jun N-terminal protein kinase pathway normally activated at G(2)/M. Mol Cell Biol 19: 8469-8478.
41. Yan CY, Greene LA. 1998. Prevention of PC12 cell death by N-acetylcysteine requires activation of the Ras pathway. J Neurosci 18: 4042-4049.
42. Zachwieja J, Zaniew M, Bobkowski W, Stefaniak E, Warzywoda A, Ostalska-Nowicka D, Dobrowolska-Zachwieja A, Lewandowska-Stachowiak M, Siwinska A. 2005. Beneficial in vitro effect of N-acetyl-cysteine on oxidative stress and apoptosis. Pediatr Nephrol 20: 725-731.
43. Zafarullah M, Li WQ, Sylvester J, Ahmad M. 2003. Molecular mechanisms of N-acetylcysteine actions. Cell Mol Life Sci 60: 6-20.
44. Zhang F-X, Chen M-L, Yang B, Cao K-J. 2009. N-acetylcysteine blocked hypoxia-reoxygenation induced apoptosis through ROS-p38 MAPK signaling pathway in neonatal rat cardiomyocytes. J Geriatr Cardiol 6: 168-172.