Icaritin induces apoptosis of HepG2 cells via the JNK1 signaling pathway independent of the estrogen receptor

Planta Medica

Volumn 76, Issue 16, 2010, Pages 1834-1839

  He, Jun ^a   Wang, Yan ^a   Duan, Fei ^a   Jiang, Hao ^a   Chen, Min Feng ^c   Tang, Si Yuan ^b  

^a UNIVERSITY OF SOUTH CHINA   (China)

^b CENTRAL SOUTH UNIVERSITY   (China)

^c CENTRAL SOUTH UNIVERSITY   (China)

Author keywords

apoptosis; Berberidaceae; cJun Nterminal kinase; Epimedium; HepG2 cells; icaritin

Indexed keywords

ANTHRA[1,9 CD]PYRAZOL 6(2H) ONE; CASPASE 3; ESTROGEN RECEPTOR; FLAVONOID; FULVESTRANT; ICARITIN; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN BAX; PROTEIN BCL 2; STRESS ACTIVATED PROTEIN KINASE 1; SYNAPTOPHYSIN; UNCLASSIFIED DRUG;

APOPTOSIS; ARTICLE; CELL STRAIN HEPG2; CONTROLLED STUDY; ENZYME ACTIVATION; HUMAN; HUMAN CELL; LIVER CELL CARCINOMA; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION;

ANTHRACENES; ANTINEOPLASTIC AGENTS, PHYTOGENIC; APOPTOSIS; BCL-2-ASSOCIATED X PROTEIN; CARCINOMA, HEPATOCELLULAR; CASPASE 3; DRUGS, CHINESE HERBAL; EPIMEDIUM; FLAVONOIDS; HEP G2 CELLS; HUMANS; JNK MITOGEN-ACTIVATED PROTEIN KINASES; LIVER NEOPLASMS; MITOGEN-ACTIVATED PROTEIN KINASES; PHYTOTHERAPY; PROTO-ONCOGENE PROTEINS C-BCL-2; RECEPTORS, ESTROGEN; SIGNAL TRANSDUCTION;

BERBERIDACEAE; EPIMEDIUM;

EID: 78349265773     PISSN: 00320943     EISSN: 14390221     Source Type: Journal    
DOI: 10.1055/s-0030-1250042     Document Type: Article

References (44)

1. Mendizabal M, Reddy K R. Current management of hepatocellular carcinoma. Med Clin North Am 2009 93 885-900
2. Rampone B, Schiavone B, Martino A, Viviano C, Confuorto G. Current management strategy of hepatocellular carcinoma. World J Gastroenterol 2009 15 3210-3216
3. Tamvakopoulos C, Dimas K, Sofianos Z D., Hatziantoniou S, Han Z, Liu Z L., Wyche J H., Pantazis P. Metabolism and anticancer activity of the curcumin analogue, dimethoxycurcumin. Clin Cancer Res 2007 13 1269-1277
4. Kunnumakkara A B., Guha S, Krishnan S, Diagaradjane P, Gelovani J, Aggarwal B B. Curcumin potentiates antitumor activity of gemcitabine in an orthotopic model of pancreatic cancer through suppression of proliferation, angiogenesis, and inhibition of nuclear factor-kappaB-regulated gene products. Cancer Res 2007 67 3853-3861
5. Hong J H., Ahn K S., Bae E, Jeon S S., Choi H Y. The effects of curcumin on the invasiveness of prostate cancer in vitro and in vivo. Prostate Cancer Prostatic Dis 2006 9 147-152
6. Wang L, Tang Z Y., Qin L X., Wu X F., Sun H C., Xue Q, Ye S L. High-dose and long-term therapy with interferon-alfa inhibits tumor growth and recurrence in nude mice bearing human hepatocellular carcinoma xenografts with high metastatic potential. Hepatology 2000 32 43-48
7. Cheng Y L., Chang W L., Lee S C., Liu Y G., Chen C J., Lin S Z., Tsai N M., Yu D S., Yen C Y., Harn H J. Acetone extract of Angelica sinensis inhibits proliferation of human cancer cells via inducing cell cycle arrest and apoptosis. Life Sci 2004 75 1579-1594
8. Yano H, Mizoguchi A, Fukuda K, Haramaki M, Ogasawara S, Momosaki S, Kojiro M. The herbal medicine sho-saiko-to inhibits proliferation of cancer cell lines by inducing apoptosis and arrest at the G0/G1phase. Cancer Res 1994 54 448-454
9. Kao S T., Yeh C C., Hsieh C C., Yang M D., Lee M R., Liu H S., Lin J G. The Chinese medicine Bu-Zhong-Yi-Qi-Tang inhibited proliferation of hepatoma cell lines by inducing apoptosis via G0/G1 arrest. Life Sci 2001 69 1485-1496
10. Lee S M., Li M L., Tse Y C., Leung S C., Lee M M., Tsui S K., Fung K P., Lee C Y., Waye M M. Paeoniae Radix, a Chinese herbal extract, inhibits hepatoma cell growth by inducing apoptosis in a p53 independent pathway. Life Sci 2002 71 2267-2277
11. Wang Z, Zhang X, Wang H, Qi L, Lou Y. Neuroprotective effects of icaritin against beta amyloid-induced neurotoxicity in primary cultured rat neuronal cells via estrogen-dependent pathway. Neuroscience 2007 145 911-922
12. Wang Z, Wang H, Wu J, Zhu D, Zhang X, Ou L, Yu Y, Lou Y. Enhanced co-expression of beta-tubulin III and choline acetyltransferase in neurons from mouse embryonic stem cells promoted by icaritin in an estrogen receptor-independent manner. Chem Biol Interact 2009 179 375-385
13. Wo Y B., Zhu D Y., Hu Y, Wang Z Q., Liu J, Lou Y J. Reactive oxygen species involved in prenylflavonoids, icariin and icaritin, initiating cardiac differentiation of mouse embryonic stem cells. J Cell Biochem 2008 103 1536-1550
14. Zhu D Y., Lou Y J. Inducible effects of icariin, icaritin, and desmethylicaritin on directional differentiation of embryonic stem cells into cardiomyocytes in vitro. Acta Pharmacol Sin 2005 26 477-485
15. Zhang G, Qin L, Sheng H, Wang X L., Wang Y X., Yeung D K., Griffith J F., Yao X S., Xie X H., Li Z R., Lee K M., Leung K S. A novel semisynthesized small molecule icaritin reduces incidence of steroid-associated osteonecrosis with inhibition of both thrombosis and lipid-deposition in a dose-dependent manner. Bone 2009 44 345-356
16. Huang J, Yuan L, Wang X, Zhang T L., Wang K. Icaritin and its glycosides enhance osteoblastic, but suppress osteoclastic, differentiation and activity in vitro. Life Sci 2007 81 832-840
17. Huang X, Zhu D, Lou Y. A novel anticancer agent, icaritin, induced cell growth inhibition, G1 arrest and mitochondrial transmembrane potential drop in human prostate carcinoma PC-3 cells. Eur J Pharmacol 2007 564 26-36
18. Liu J, Ye H, Lou Y. Determination of rat urinary metabolites of icariin in vivo and estrogenic activities of its metabolites on MCF-7 cells. Pharmazie 2005 60 120-125
19. Tang S Y., Xie H, Yuan L Q., Luo X H., Huang J, Cui R R., Zhou H D., Wu X P., Liao E Y. Apelin stimulates proliferation and suppresses apoptosis of mouse osteoblastic cell line MC3T3-E1 via JNK and PI3-K/Akt signaling pathways. Peptides 2007 28 708-718
20. Wang Q P., Xie H, Yuan L Q., Luo X H., Li H, Wang D, Meng P, Liao E Y. Effect of progesterone on apoptosis of murine MC3T3-E1 osteoblastic cells. Amino Acids 2009 36 57-63
21. Xie H, Tang S Y., Li H, Luo X H., Yuan L Q., Wang D, Liao E Y. L-carnitine protects against apoptosis of murine MC3T3-E1 osteoblastic cells. Amino Acids 2008 35 419-423
22. Xie H, Yuan L Q., Luo X H., Huang J, Cui R R., Guo L J., Zhou H D., Wu X P., Liao E Y. Apelin suppresses apoptosis of human osteoblasts. Apoptosis 2007 12 247-254
23. Xie H, Tang L L., Luo X H., Wu X Y., Wu X P., Zhou H D., Yuan L Q., Liao E Y. Suppressive effect of dexamethasone on TIMP-1 production involves murine osteoblastic MC3T3-E1 cell apoptosis. Amino Acids 2010 38 1145-1153
24. Kuo Y C., Kuo P L., Hsu Y L., Cho C Y., Lin C C. Ellipticine induces apoptosis through p53-dependent pathway in human hepatocellular carcinoma HepG2 cells. Life Sci 2006 78 2550-2557
25. Xie H, Tang S Y., Luo X H., Huang J, Cui R R., Yuan L Q., Zhou H D., Wu X P., Liao E Y. Insulin-like effects of visfatin on human osteoblasts. Calcif Tissue Int 2007 80 201-210
26. Xie H, Tang S Y., Cui R R., Huang J, Ren X H., Yuan L Q., Lu Y, Yang M, Zhou H D., Wu X P., Luo X H., Liao E Y. Apelin and its receptor are expressed in human osteoblasts. Regul Pept 2006 134 118-125
27. Xie H, Yang B, Zhou X M., Song F L., Li J M., Zhou K, Hu W, Peng Y Q., Tang S Y., Yuan L Q., Xiong S Y., Liao X B. L-carnitine and taurine synergistically inhibit the proliferation and osteoblastic differentiation of vascular smooth muscle cells. Acta Pharmacol Sin 2010 31 289-296
28. Liao X B., Peng Y Q., Zhou X M., Yang B, Zheng Z, Liu L M., Song F L., Li J M., Zhou K, Meng J C., Yuan L Q., Xie H. Taurine restores Axl/Gas6 expression in vascular smooth muscle cell calcification model. Amino Acids in press
29. Li H, Xie H, Liu W, Hu R, Huang B, Tan Y F., Xu K, Sheng Z F., Zhou H D., Wu X P., Luo X H. A novel microRNA targeting HDAC5 regulates osteoblast differentiation in mice and contributes to primary osteoporosis in humans. J Clin Invest 2009 119 3666-3677
30. Kim B R., Hu R, Keum Y S., Hebbar V, Shen G, Nair S S., Kong A N. Effects of glutathione on antioxidant response element-mediated gene expression and apoptosis elicited by sulforaphane. Cancer Res 2003 63 7520-7525
31. Ip Y T., Davis R J. Signal transduction by the c-Jun N-terminal kinase (JNK)from inflammation to development. Curr Opin Cell Biol 1998 10 205-219
32. Kumagae Y, Zhang Y, Kim O J., Miller C A. Human c-Jun N-terminal kinase expression and activation in the nervous system. Brain Res Mol Brain Res 1999 67 10-17
33. Ricchi M, Bertolotti M, Anzivino C, Carulli L, Canedi I, Bormioli M L., Tiozzo R, Croce M A., Lonardo A, Carulli N, Loria P. 17 Beta-estradiol prevents cytotoxicity from hydrophobic bile acids in HepG2 and WRL-68 cell cultures. J Gastroenterol Hepatol 2006 21 894-901
34. Adams J M., Cory S. The Bcl-2 protein family: arbiters of cell survival. Science 1998 281 1322-1326
35. Li X, Darzynkiewicz Z. Cleavage of poly (ADP-ribose) polymerase measured in situ in individual cells: relationship to DNA fragmentation and cell cycle position during apoptosis. Exp Cell Res 1998 25 125-132
36. Orth K, Chinnaiyan A M., Garth M, Froelich C J., Dixit V M. The CED-3/ICE-like protease Mch2 is activated during apoptosis and cleaves the death substrate lamin A. J Biol Chem 1996 271 16443-16446
37. Gustafsson J A. Estrogen receptor betaa new dimension in estrogen mechanism of action. J Endocrinol 1999 163 379-383
38. Solakidi S, Psarra A M., Sekeris C E. Differential subcellular distribution of estrogen receptor isoforms: localization of ERalpha in the nucleoli and ERbeta in the mitochondria of human osteosarcoma SaOS-2 and hepatocarcinoma HepG2 cell lines. Biochim Biophys Acta 2005 1745 382-392
39. Wakeling A E., Dukes M, Bowler J. A potent specific pure antiestrogen with clinical potential. Cancer Res 1991 51 3867-3873
40. Blüthgen N, Legewie S. Systems analysis of MAPK signal transduction. Essays Biochem 2008 45 95-107
41. Chang L, Karin M. Mammalian MAP kinase signalling cascades. Nature 2001 410 37-40
42. Ma F Y., Flanc R S., Tesch G H., Han Y, Atkins R C., Bennett B L., Friedman G C., Fan J H., Nikolic-Paterson D J. A pathogenic role for c-Jun amino-terminal kinase signaling in renal fibrosis and tubular cell apoptosis. J Am Soc Nephrol 2007 18 472-484
43. Liu J, Minemoto Y, Lin A. c-Jun N-terminal protein kinase 1 (JNK1), but not JNK2, is essential for tumor necrosis factor alpha-induced c-Jun kinase activation and apoptosis. Mol Cell Biol 2004 24 10844-10856
44. Wong S P., Shen P, Lee L, Li J, Yong E L. Pharmacokinetics of prenylflavonoids and correlations with the dynamics of estrogen action in sera following ingestion of a standardized Epimedium extract. J Pharm Biomed Anal 2009 50 216-223