A new tagged-TEV protease: Construction, optimisation of production, purification and test activity

Protein Expression and Purification

Volumn 75, Issue 1, 2011, Pages 75-82

  Miladi, Baligh ^a   Bouallagui, Hassib ^b   Dridi, Cyrine ^a   El Marjou, Ahmed ^c   Boeuf, Guilhem ^a   Di Martino, Patrick ^d   Dufour, Florence ^a   Elm'Selmi, Abdellatif ^a  

^a Ecole de Biologie Industrielle   (France)

^b UNIVERSITY OF CARTHAGE   (Tunisia)

^c INSTITUT CURIE   (France)

^d UNIVERSITÉ DE CERGY PONTOISE   (France)

Author keywords

Affinity chromatography; High level of production; Solubility; Streptag II TEV

Indexed keywords

HIS HIS HIS HIS HIS HIS; HIS-HIS-HIS-HIS-HIS-HIS; HISTIDINE; HYBRID PROTEIN; OLIGOPEPTIDE; PROTEINASE; TEV PROTEASE; VIRUS PROTEIN;

ARTICLE; ENZYMOLOGY; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PLANT VIRUS; PLASMID; TOBACCO; VIROLOGY;

BASE SEQUENCE; CLONING, MOLECULAR; ENDOPEPTIDASES; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HISTIDINE; MOLECULAR SEQUENCE DATA; OLIGOPEPTIDES; PLANT VIRUSES; PLASMIDS; RECOMBINANT FUSION PROTEINS; TOBACCO; VIRAL PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 78049317940     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.08.012     Document Type: Article

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