Self-cleavage of fusion protein in vivo using TEV protease to yield native protein

Protein Science

Volumn 14, Issue 4, 2005, Pages 936-941

  Shih, Yan Ping ^a   Wu, Hui Chung ^a   Hu, Su Ming ^a   Wang, Ting Fang ^a   Wang, Andrew H J ^a  

^a INSTITUTE OF BIOLOGICAL CHEMISTRY   (Taiwan)

Author keywords

Fusion protein approach; Sticky end PCR; Tobacco etch virus protease

Indexed keywords

HYBRID PROTEIN; RECOMBINANT PROTEIN; VIRUS ENZYME;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ARTICLE; ENZYME ACTIVITY; ESCHERICHIA COLI; EUKARYOTIC CELL; EXPRESSION VECTOR; GENE CONSTRUCT; GENE IDENTIFICATION; IN VIVO STUDY; MOLECULAR CLONING; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PROCESSING; PROTEIN SYNTHESIS; TECHNIQUE; TOBACCO VIRUS; VIRUS;

AMINO ACID SEQUENCE; CARRIER PROTEINS; CLONING, MOLECULAR; ENDOPEPTIDASES; HISTIDINE; OLIGOPEPTIDES; RECOMBINANT FUSION PROTEINS;

EID: 15244363693     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.041129605     Document Type: Article

References (12)

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