Structural and biochemical studies of a fluoroacetyl-CoA-specific thioesterase reveal a molecular basis for fluorine selectivity

Biochemistry

Volumn 49, Issue 43, 2010, Pages 9269-9279

  Weeks, Amy M ^a   Coyle, Scott M ^a   Jinek, Martin ^a   Doudna, Jennifer A ^a   Chang, Michelle C Y ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

[CARBONYL; ACETYL-COA; ACTIVE SITE; BIOCHEMICAL STUDIES; CATALYTIC RESIDUE; COENZYME A; DRIVING FORCES; ENZYMATIC SYSTEM; FLUORINE SUBSTITUTION; FLUOROACETATE; HYDROPHOBIC BINDING; IN-VIVO; MOLECULAR BASIS; MOLECULAR INSIGHTS; ORGANOFLUORINE; STRUCTURAL STUDIES; SUBSTRATE BINDING; SUBSTRATE SELECTIVITY; THIOESTERASES; THIOESTERS;

SULFUR COMPOUNDS;

FLUORINE;

ACETYL COENZYME A; FLUORINE; FLUOROACETIC ACID; FLUOROACETYL COENZYME A SPECIFIC THIOESTERASE; UNCLASSIFIED DRUG;

ARTICLE; CRYSTAL STRUCTURE; ESCHERICHIA COLI; GENE SYNTHESIS; HYDROLYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROTEIN STRUCTURE; SITE DIRECTED MUTAGENESIS; STREPTOMYCES; STREPTOMYCES CATTLEYA; X RAY CRYSTALLOGRAPHY;

ACETYL COENZYME A; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; FLUORINE; KINETICS; PROTEIN BINDING; PROTEIN CONFORMATION; STREPTOMYCES; SUBSTRATE SPECIFICITY; THERMODYNAMICS; THIOLESTER HYDROLASES; WATER;

STREPTOMYCES CATTLEYA;

EID: 78049301731     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi101102u     Document Type: Article

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