A sensitive and robust method for quantification of intracellular short-chain coenzyme A esters

Analytical Biochemistry

Volumn 328, Issue 1, 2004, Pages 51-59

  Shimazu, Mark ^a   Vetcher, Leandro ^a   Galazzo, Jorge L ^a   Licari, Peter ^a   Santi, Daniel V ^a  

^a KOSAN BIOSCIENCES INC   (United States)

Author keywords

Bacterial cell extracts; CoA esters; Metabolic quenching; Polyketides

Indexed keywords

COENZYMES; CYTOLOGY; ESTERS; EXTRACTION; FLUORESCENCE; METABOLISM; METABOLITES; NUCLEOTIDES; QUENCHING; SILICONES; TRICHLOROACETIC ACID;

ADENINE NUCLEOTIDE; BACTERIAL CELLS; CELL METABOLITES; FLUORESCENCE DETECTOR; POLYKETIDES; POSTCOLUMN REACTION; SALT-FREE SOLUTION; TRI-N-OCTYLAMINE;

CELLS;

2 BROMOACETALDEHYDE; ACETALDEHYDE; ACYL COENZYME A; COENZYME A; FREON; OCTYLAMINE; SILICONE OIL; TRICHLOROACETIC ACID; UNCLASSIFIED DRUG;

ANALYTIC METHOD; AQUEOUS SOLUTION; ARTICLE; BACTERIAL CELL; BACTERIUM ISOLATION; CENTRIFUGATION; CONTROLLED STUDY; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; METABOLISM; NONHUMAN; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; SERODIAGNOSIS;

BACTERIA (MICROORGANISMS);

EID: 1842736537     PISSN: 00032697     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ab.2004.01.025     Document Type: Article

References (31)

1. Murli S., Kennedy J., Dayem L.C., Carney J.R., Kealey J.T. Metabolic engineering of Escherichia coli for improved 6-deoxyerythronolide B production. J. Ind. Microbiol. Biotechnol. 30:2003;500-509.
2. Rodriguez E., McDaniel R. Combinatorial biosynthesis of antimicrobials and other natural products. Curr. Opin. Microbiol. 4:2001;526-534.
3. Carreras C.W., Santi D.V. Engineering of polyketide synthases to produce novel polyketides. Curr. Opin. Biotechnol. 9:1998;403-411.
4. Jackowski S., Rock C.O. Regulation of coenzyme A biosynthesis. J. Bacteriol. 148:1981;926-932.
5. Dayem L.C., Carney J.R., Santi D.V., Pfeifer B.A., Khosla C., Kealey J.T. Metabolic engineering of a methylmalonyl-CoA mutase-epimerase pathway for complex polyketide biosynthesis in Escherichia coli. Biochemistry. 41:2002;5193-5201.
6. Pogolotti J.A.L., Santi D.V. High-pressure liquid chromatography- ultraviolet analysis of intracellular nucleotides. Anal. Biochem. 126:1982;335-345.
7. Buchholz A., Takors R., Wandrey C. Quantification of intracellular metabolites in Escherichia coli K12 using liquid chromatographic-electrospray ionization tandem mass spectrometric techniques. Anal. Biochem. 295:2001;129-137.
8. Tamyakopoulos C.S., Anderson V.E. Detection of acyl-coenzyme A thioester intermediates of fatty acid beta-oxidation as the N-acylglycines by negative-ion chemical ionization gas chromatography-mass spectrometry. Anal. Biochem. 200:1992;381-387.
9. Kochetkov N.K., Shibaev V.N., Kost A.A. New reaction of adenine and cytosine derivatives, potentially useful for nucleic acid modifications. Tetrahedron Lett. 22:1971;1993-1996.
10. Barrio J.R., Secrist J.A., Leonard N.J. Fluorescent adenosine and cytidine derivatives. Biochem. Biophys. Res. Commun. 46:1972;597-604.
11. Larson T.R., Graham I.A. Technical advance: a novel technique for the sensitive quantification of acyl CoA esters from plant tissues. Plant J. 25:2001;115-125.
12. Fujimori H., Sasaki T., Hibi K., Senda M., Yoshioka M. Measurement of adenine nucleotide levels with an adenine analyser as an index of freshness of porgy. J. Chromatogr. 528:1990;305-314.
13. Chapman A.G., Atkinson D.E. Adenine nucleotide concentrations and turnover rates. Their correlation with biological activity in bacteria and yeast. Adv. Microb. Physiol. 15:1977;253-306.
14. Stanley P.E. Extraction of adenosine triphosphate from microbial and somatic cells. Methods Enzymol. 133:1986;14-22.
15. Karl D.M. Cellular nucleotide measurements and applications in microbial ecology. Microbiol. Rev. 44:1980;739-796.
16. Emmerling M., Bailey J.E. Glucose catabolism of Escherichia coli strains with increased activity and altered regulation of key glycolytic enzymes. Metab. Eng. 1:1999;117-127.
17. C. Hwang, The analysis of intracellular and extracelluar redox states in cell culture, Ph.D. Thesis, Biochemical Engineering, Massachusetts Institute of Technology (1992)
18. Hwang C., Sinskey A.J., Lodish H.F. Oxidized redox state of glutathione in the endoplasmic reticulum. Science. 257:1992;1496-1502.
19. Gerth K., Bedorf N., Hofle G., Irschik H., Reichenbach H. Epothilones A and B: antifungal and cytotoxic compounds from Sorangium cellulosum (Myxobacteria). Production, physico-chemical and biological properties. J. Antibiot. (Tokyo). 49:1996;560-563.
20. Zusman D.R., Krotoski D.M., Cumsky M. Chromosome replication in Myxococcus xanthus. J. Bacteriol. 133:1978;122-129.
21. Khym J.X. An analytical system for rapid separation of tissue nucleotides at low pressures on conventional anion exchangers. Clin. Chem. 21:1975;1245-1252.
22. Bischofberger N., Waldmann H., Saito T., Simon E.S., Lees W., Bednarski M.D., Whitesides G.M. Synthesis of analogues of 1,3-dihdroxyacetone phosphate and glyceraldehyde 3-phosphate for use in studies of fructose-1,6-diphosphate aldolase. J. Org. Chem. 53:1988;3457-3465.
23. Biernal J., Ciesiolka J., Gornicki P., Adamiak R.W., Kryzosiak W.J., Wiewiorowski M. New observations concerning the chloroacetaldehyde reaction with some tRNA constituents. Stable intermediates, kinetics and selectivity of the reaction. Nucleic Acids Res. 5:1978;789-804.
24. Kayasuga-Mikado K., Hashimoto T., Negishi T., Negishi K., Hayatsu H. Modification of adenine and cytosine derivatives with bromoacetaldehyde. Chem. Pharm. Bull. 28:1980;932-938.
25. Zhang Y., Geiger J.D., Lautt W.W. Improved high-pressure liquid chromatographic fluorometric assay for measurement of adenosine in plasma. Am. J. Physiol. 260:1991;G658-G664.
26. Stadtman E.R. Preparation and assay of acyl Coenzyme A and other thiol esters; use of hydroxylamine. Methods Enzymol. 3:1957;931-941.
27. Chapman A.G., Fall L., Atkinson D.E. Adenylate energy charge in Escherichia coli during growth and starvation. J. Bacteriol. 108:1971;1072-1086.
28. Vallari D.S., Jackowski S., Rock C.O. Regulation of pantothenate kinase by Coenzyme A and its thioesters. J. Biol. Chem. 262:1987;2468-2471.
29. Miksic J.R., Brown P.R. Reactions of reduced nicotinamide adenine dinucleotide in acid: studies by reversed-phase high-pressure liquid chromatography. Biochemistry. 17:1978;2234-2238.
30. Cole H.A., Wimpenny J.W.T., Hughes D.E. The ATP pools in Escherichia coli I. Measurement of the pools using modified luciferase assay. Biochim. Biophys. Acta. 143:1967;445-453.
31. Lundin A., Thore A. Comparison of methods for extraction of bacterial adenine nucleotides determined by firefly assay. Appl. Microbiol. 30:1975;713-721.