Site-specific saturation mutagenesis on residues 132 and 450 of candida rugosa LIP2 enhances catalytic efficiency and alters substrate specificity in various chain lengths of triglycerides and esters

Journal of Agricultural and Food Chemistry

Volumn 58, Issue 20, 2010, Pages 10899-10905

  Yen, Chih Chung ^a,b   Malmis, Conmar C ^a,c,e   Lee, Guan Chiun ^d   Lee, Li Chiun ^a   Shaw, Jei Fu ^a,c  

^a INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

^b NATIONAL TAIWAN UNIVERSITY   (Taiwan)

^c NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

^d NATIONAL TAIWAN NORMAL UNIVERSITY   (Taiwan)

^e ACADEMIA SINICA   (Taiwan)

Author keywords

Candida rugosa LIP2; Mutants; substrate specificity; Pichia pastoris; Site specific saturation mutagenesis

Indexed keywords

CANDIDA RUGOSA; CATALYTIC EFFICIENCIES; CATALYTIC VERSATILITY; DEGENERATE PRIMERS; ENHANCED CATALYTIC ACTIVITY; LIPASE ACTIVITY; MUTANTS SUBSTRATE SPECIFICITY; OPTIMAL TEMPERATURE; PH STABILITY; PICHIA PASTORIS; POINT MUTATIONS; POTENTIAL APPLICATIONS; SATURATION MUTAGENESIS; SITE-SPECIFIC; SITE-SPECIFIC SATURATION MUTAGENESIS; SUBSTRATE SPECIFICITY; TRIBUTYRIN; WILD TYPES;

AMINO ACIDS; CANDIDA; CATALYST ACTIVITY; ESTERIFICATION; MUTAGENESIS; PH EFFECTS; SUBSTRATES; YEAST;

ESTERS;

CANDIDA RUGOSA; PICHIA PASTORIS;

ESTER; FUNGAL PROTEIN; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE;

AMINO ACID SUBSTITUTION; BIOCATALYSIS; CANDIDA; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; BIOCATALYSIS; CANDIDA; ESTERS; FUNGAL PROTEINS; LIPASE; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY; TRIGLYCERIDES;

EID: 78049287825     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf1004034     Document Type: Article

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