Multiple mutagenesis of non-universal serine codons of the Candida rugosa LIP2 gene and biochemical characterization of purified recombinant LIP2 lipase overexpressed in Pichia pastoris

Biochemical Journal

Volumn 366, Issue 2, 2002, Pages 603-611

  Lee, Guan Chiun ^a   Lee, Li Chiun ^a   Sava, Vasyl ^a   Shaw, Jei Fu ^a  

^a INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

Author keywords

Isoforms; Recombinant lipase; Site directed mutagenesis; Substrate specificity

Indexed keywords

ALCOHOLS; AMINO ACIDS; ENZYMES; ESTERIFICATION; ESTERS; HYDROPHOBICITY; MUTAGENESIS; PH EFFECTS;

LIPOLYSIS;

GENES;

4 NITROPHENYLESTER; ALCOHOL DERIVATIVE; AMINO ACID; CHOLESTEROL ESTER; ESTER DERIVATIVE; ESTERASE; FATTY ACID; FUNGAL ENZYME; LAURIC ACID DERIVATIVE; MYRISTIC ACID; OCTADECANOL; PROPANOL; RECOMBINANT ENZYME; SERINE; TRIACYLGLYCEROL; TRIACYLGLYCEROL LIPASE; TRIBUTYRIN; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; BINDING SITE; BIOCHEMISTRY; CANDIDA RUGOSA; CATALYSIS; CODON; COMMERCIAL PHENOMENA; CONTROLLED STUDY; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME RELEASE; ENZYME SPECIFICITY; ESTERIFICATION; FUNGAL GENE; GENE; HEATING; HYDROPHOBICITY; LIP2 GENE; LIPOLYSIS; MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; SITE DIRECTED MUTAGENESIS; TEMPERATURE; TIME;

BASE SEQUENCE; BINDING SITES; CANDIDA; CARBOXYLIC ESTER HYDROLASES; CLONING, MOLECULAR; CODON; DNA PRIMERS; FUNGAL PROTEINS; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PICHIA; POLYMERASE CHAIN REACTION; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SERINE; SUBSTRATE SPECIFICITY;

CANDIDA; CANDIDA RUGOSA; PICHIA; PICHIA PASTORIS;

EID: 0036714663     PISSN: 02646021     EISSN: None     Source Type: Journal    
DOI: 10.1042/BJ20020404     Document Type: Article

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