Codon optimization of Candida rugosa lip1 gene for improving expression in Pichia pastoris and biochemical characterization of the purified recombinant LIP1 lipase

Journal of Agricultural and Food Chemistry

Volumn 54, Issue 3, 2006, Pages 815-822

  Chang, Shu Wei ^a,b   Lee, Guan Chiun ^c   Shaw, Jei Fu ^a,b,d  

^a NATIONAL TAIWAN OCEAN UNIVERSITY   (Taiwan)

^b INSTITUTE OF PLANT AND MICROBIAL BIOLOGY   (Taiwan)

^c MING CHUAN UNIVERSITY   (Taiwan)

^d NATIONAL CHUNG HSING UNIVERSITY   (Taiwan)

Author keywords

Candida rugosa lipase; Codon optimization; Isoforms; Pichia pastoris

Indexed keywords

FUNGAL DNA; ISOENZYME; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ARTICLE; CANDIDA; CHEMISTRY; CODON; ENZYME SPECIFICITY; ENZYMOLOGY; GENE EXPRESSION; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PICHIA; POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS; TEMPERATURE;

BASE SEQUENCE; CANDIDA; CODON; DNA, FUNGAL; GENE EXPRESSION; HYDROGEN-ION CONCENTRATION; ISOENZYMES; LIPASE; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; PICHIA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SUBSTRATE SPECIFICITY; TEMPERATURE;

CANDIDA RUGOSA; HEPATITIS A VIRUS; PICHIA PASTORIS;

EID: 33244490497     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1175/JAS3661.1     Document Type: Article

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