Tyramide signal amplification for analysis of kinase activity by intracellular flow cytometry

Cytometry Part A

Volumn 77, Issue 11, 2010, Pages 1020-1031

  Clutter, Matthew R ^a,b   Heffner, Garrett C ^b   Krutzik, Peter O ^a   Sachen, Kacey L ^b   Nolan, Garry P ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b STANFORD UNIVERSITY   (United States)

Author keywords

Background; Catalyzed reporter deposition; Cell signaling; Cell based assay; Enzymatic amplification staining; Flow cytometry; Intracellular flow cytometry; Phospho flow; Signal transduction; Tyramide signal amplification

Indexed keywords

GAMMA INTERFERON; INTERLEUKIN 6; MITOGEN ACTIVATED PROTEIN KINASE; PHOSPHOPROTEIN; TYRAMINE;

ANIMAL; ARTICLE; BAGG ALBINO MOUSE; CELL SEPARATION; CELL STRAIN U937; CHEMISTRY; CYTOLOGY; DRUG EFFECT; FEMALE; FLOW CYTOMETRY; HUMAN; LEUKEMIA; LEUKEMIA CELL LINE; LIMIT OF DETECTION; LYMPHOMA; METABOLISM; METHODOLOGY; MOUSE; NUCLEIC ACID AMPLIFICATION; PHOSPHORYLATION; PROTEIN BINDING; SPLEEN; STAINING; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; CELL SEPARATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; FEMALE; FLOW CYTOMETRY; HUMANS; INTERFERON-GAMMA; INTERLEUKIN-6; JURKAT CELLS; LEUKEMIA; LIMIT OF DETECTION; LYMPHOMA; MICE; MICE, INBRED BALB C; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PHOSPHOPROTEINS; PHOSPHORYLATION; PROTEIN BINDING; SPLEEN; STAINING AND LABELING; TYRAMINE; U937 CELLS;

EID: 77958569391     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.20970     Document Type: Article

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