Rapid synthesis of biotin-, digoxigenin-, trinitrophenyl-, and fluorochrome-labeled tyramides and their application for in situ hybridization using CARD amplification

Journal of Histochemistry and Cytochemistry

Volumn 46, Issue 6, 1998, Pages 771-777

  Hopman, Anton H N ^a   Ramaekers, Frans C S ^a   Speel, Ernst J M ^a,b  

^a MAASTRICHT UNIVERSITY   (Netherlands)

^b UNIVERSITY HOSPITAL ZURICH   (Switzerland)

Author keywords

(F)ISH; DNA probes; Enzyme cytochemistry; Fluorescent labeling; Horseradish peroxidase; Interphase cytogenetics; Tyramide conjugates

Indexed keywords

BIOTIN; DIGOXIGENIN; FLUOROCHROME; HORSERADISH PEROXIDASE; ISOPROPAMIDE IODIDE; N,N DIMETHYLFORMAMIDE; TRINITROPHENYL;

ARTICLE; CYTOCHEMISTRY; CYTOGENETICS; DNA PROBE; GENE TARGETING; HUMAN; HUMAN CELL; IN SITU HYBRIDIZATION; INTERPHASE; PRIORITY JOURNAL;

ARMORACIA RUSTICANA;

EID: 0031775655     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215549804600611     Document Type: Article

References (24)

1. Adams J (1992) Biotin amplification of biotin and horseradish peroxidase signals in histochemical stains. J Histochem Cytochem 40:1457-1463
2. Berghorn KA, Bonnett JH, Hoffman GE (1994) cFos immunoreactivity is enhanced with biotin amplification. J Histochem Cytochem 42:1635-1642
3. Bobrow MN, Harris TD, Shaughnessy KJ, Litt GJ (1989) Catalyzed reporter deposition, a novel method of signal amplification. J Immunol Methods 125:279-285
4. Bobrow MN, Shaughnessy KJ, Litt GJ (1991) Catalyzed reporter deposition, a novel method of signal amplification. J Immunol Methods 137:103-112
5. Bubeník J, Baresová M, Viklicky V, Jakoubková J, Sainerová H, Donner J (1973) Established cell line of urinary bladder carcinoma (T24) containing tumour-specific antigen. Int J Cancer 11:765-773
6. Buroker NR, Bestwick R, Haight G, Magenis RE, Litt M (1987) A hypervariable repeated sequence on human chromosome 1p36. Hum Genet 77:175-181
7. Chao J, DeBiasio R, Zhu Z, Giuliano KA, Schmidt BF (1996) Immunofluorescence signal amplification by the enzyme-catalyzed deposition of a fluorescent reporter substrate (CARD). Cytometry 23:48-53
8. Cooke JH, Handle J (1979) Cloning of human satellite III DNA: different components are on different chromosomes. Nucleic Acids Res 6:3177-3197
9. Graham RC, Karnovsky MJ (1966) The early stages of absorption of injected horseradish peroxidase in the proximal tubules of mouse kidney: ultrastructural cytochemistry by a new technique. J Histochem Cytochem 14:291-302
10. Hopman AHN, Ramaekers FCS, Raap AK, Beck JLM, Devilee P, Van der Ploeg M, Vooijs GP (1988) In situ hybridization as a tool to study numerical chromosome aberrations in solid bladder tumors. Histochemistry 89:307-316
11. Hopman AHN, Wiegant J, Raap AK, Landegent JE, Van der Ploeg M, Van Duijn P (1986a) Bi-color detection of two target DNAs by non-radioactive in situ hybridization. Histochemistry 85:1-4
12. Hopman AHN, Wiegant J, Tesser GI, Van Duijn P (1986b) A non-radioactive in situ hybridization method based on mercurated nucleic acid probes and sulfhydryl-hapten ligands. Nucleic Acids Res 14:6471-6488
13. Kerstens HJM, Poddighe PJ, Hanselaar AGJM (1995) A novel in situ hybridization signal amplification method, based on the deposition of biotinylated tyramine. J Histochem Cytochem 43:347-352
14. Kerstens HLM, Poddighe PJ, Hanselaar AGJM (1994) Double-target in situ hybridization in brightfield microscopy. J Histochem Cytochem 42:1071-1077
15. Landegent JE, Jansen in de Wal N, Fisser-Groen YM, Bakker E, Ploeg van der M, Pearson PL (1986) Fine mapping of the Huntington disease linked D4S10 locus by non-radioactive in situ hybridization. Hum Genet 73:354-357
16. Little RD, Braaten DC (1989) Genomic organization of human 5S rDNA and sequence of one tandem repeat. Genomics 4:376-383
17. 35S]dithiobis(succinimidyl propionate). J Mol Biol 104:243-261
18. Raap AK, Van de Corput MPC, Vervenne RAW, Van Gijlswijk RPM, Tanke JH, Wiegant J (1995) Ultra-sensitive FISH using peroxidase-mediated deposition of biotin-or fluorochrome tyramide. Hum Mol Genet 4:529-534
19. Speel EJM, Jansen MPHM, Ramaekers FCS, Hopman AHN (1994) A novel triple-color detection procedure for brightfield microscopy, combining in situ hybridization with immunocytochemistry. J Histochem Cytochem 42:1299-1307
20. Speel EJM, Kamps M, Bonnet J, Ramaekers FCS, Hopman AHN (1993) Multicolour preparations for in situ hybridization using precipitating enzyme cytochemistry in combination with reflection contrast microscopy. Histochemistry 100:357-366
21. Speel EJM, Ramaekers FCS, Hopman AHN (1995) Cytochemical detection systems for in situ hybridization, and the combination with immunocytochemistry. "Who is still afraid of red, green and blue?" Histochem J 27:833-858
22. Speel EJM, Ramaekers FCS, Hopman AHN (1997) Sensitive multicolor fluorescence in situ hybridization using catalyzed reporter deposition (CARD) amplification. J Histochem Cytochem 45: 1439-1446
23. Van Gijlswijk RPM, Wiegant J, Vervenne R, Lasan R, Tanke JH, Raap AK (1996) Horseradish peroxidase-labeled oligonucleotides and fluorescent tyramides for rapid detection of chromosome-specific repeat sequences. Cytogenet Cell Genet 75:258-262
24. Van Gijlswijk RPM, Zijlmans HJMAA, Wiegant J, Bobrow MN, Erickson TJ, Adler KE, Tanke JH, Raap AK (1997) Fluorochrome labeled tyramides: use in immunocytochemistry and fluorescence in situ hybridization. J Histochem Cytochem 45:375-382