Pilot scale production and isolation of recombinant NAD+- and NADP+- specific formate dehydrogenases

Biotechnology and Bioengineering

Volumn 64, Issue 2, 1999, Pages 187-193

  Tishkov, Vladimir I ^a,b   Galkin, Andrey G ^a   Fedorchuk, Vladimir V ^a   Savitsky, Pavel A ^a   Rojkova, Alexandra M ^a   Gieren, Holger ^b   Kula, Maria Regina ^b  

^a MOSCOW STATE UNIVERSITY   (Russian Federation)

^b HEINRICH HEINE UNIVERSITY   (Germany)

Author keywords

Aqueous two phase extraction; Expression in E. coli; Formate dehydrogenase; Large scale cultivation; Optimization of expression; Pseudomonas sp. 101

Indexed keywords

CELL CULTURE; ESCHERICHIA COLI; EXTRACTION; PURIFICATION; SOLUBILITY;

FORMATE DEHYDROGENASE;

ENZYMES;

FORMATE DEHYDROGENASE; NICOTINAMIDE ADENINE DINUCLEOTIDE; NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

AQUEOUS SOLUTION; ARTICLE; BIOENGINEERING; BIOMASS; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SYNTHESIS; ESCHERICHIA COLI; EXTRACTION; GENETIC RECOMBINATION; HOMOGENATE; PROTEIN EXPRESSION; PSEUDOMONAS;

BIOMASS; ESCHERICHIA COLI; FERMENTATION; FORMATE DEHYDROGENASES; MUTAGENESIS, SITE-DIRECTED; PSEUDOMONAS; RECOMBINANT PROTEINS; TIME FACTORS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS SP.; PSEUDOMONAS SP. 101;

EID: 0033587795     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19990720)64:2<187::AID-BIT7>3.0.CO;2-0     Document Type: Article

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