Simple purification of the human antimicrobial peptide dermcidin (MDCD-1L) by intein-mediated expression in E. coli

Journal of Microbiology and Biotechnology

Volumn 20, Issue 2, 2010, Pages 350-355

  Hong, Inpyo ^a   Kim, Yong Seok ^b   Choi, Shin Geon ^a  

^a Kangwon National University   (South Korea)

^b Hanyang University   (South Korea)

Author keywords

Antimicrobial peptides; DCD 1L; Dermcidin; Intein; Protein expression

Indexed keywords

CHITIN; COMPLEMENTARY DNA; DERMCIDIN; HYBRID PROTEIN; INTEIN; METHIONINE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIMICROBIAL ACTIVITY; ARTICLE; DNA LIBRARY; DNA TEMPLATE; ELECTROSPRAY MASS SPECTROMETRY; ESCHERICHIA COLI; GEL ELECTROPHORESIS; HUMAN; LIQUID CHROMATOGRAPHY; MICROCOCCUS LUTEUS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; PROTEIN ANALYSIS; PROTEIN PURIFICATION; TANDEM MASS SPECTROMETRY;

ANTI-BACTERIAL AGENTS; ESCHERICHIA COLI; GENE EXPRESSION; HUMANS; INTEINS; MICROCOCCUS; PEPTIDES; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS;

MICROCOCCUS LUTEUS;

EID: 77649214723     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0907.07029     Document Type: Article

References (20)

1. Baechle, D., T. Flad, A. Cansier, H. Steffen, B. Schittek, J. Tolson, et al. 2006. Cathepsin D is present in human eccrine sweat and involved in the postsecretory processing of the antimicrobial peptide DCD-1L. J. Biol. Chem. 281: 5406-5415.
2. Bisht, G. S., D. S. Rawat, A. Kumar, R. Kumar, and S. Pasha. 2007. Antimicrobial activity of rationally designed amino terminal modified peptides. Bioorg. Med. Chem. Lett. 17: 4343-4346.
3. Chen, Y. Q., S. Q. Zhang, B. C. Li, W. Qiu, B. Jiao, J. Zhang, and Z. Y. Diao. 2008. Expression of a cytotoxic cationic antibacterial peptide in Escherichia coli using two fusion partners. Protein Expr. Purif. 57: 303-311.
4. Cipáková, I., J. Gasperík, and E. Hostinová. 2006. Expression and purification of human antimicrobial peptide, dermcidin, in Escherichia coli. Protein Expr. Purif. 45: 269-274.
5. Diao, H., C. Guo, D. Lin, and Y. Zhang. 2007. Intein-mediated expression is an effective approach in the study of betadefensins. Biochem. Biophys. Res. Commun. 357: 840-846.
6. Flad, T., R. Bogumil, J. Tolson, B. Schittek, C. Garbe, M. Deeg, C. A. Mueller, and H. Kalbacher. 2002. Detection of dermcidin-derived peptides in sweat by ProteinChip technology. J. Immunol. Methods 270: 53-62.
7. Hong, I. P., Y. S. Kim, and S. G. Choi. 2007. Recombinant expression of human cathelicidin (hCAP18/Ll37) in Pichia pastoris. Biotechnol. Lett. 29: 73-78.
8. Huang, L., J. Wang, Z. Zhong, L. Peng, H. Chen, Z. Xu, and P. Chen. 2006. Production of bioactive human beta-defensin-3 in Escherichia coli by soluble fusion expression. Biotechnol. Lett. 28: 627-632.
9. Ingham, A. B. and R. J. Moore. 2007. Recombinant production of antimicrobial peptides in heterologous microbial systems. Biotechnol. Appl. Biochem. 47: 1-9.
10. Lai, Y. P., Y. F. Peng, Y. Zuo, J. Li, J. Huang, L. F. Wang, and Z. R. Wu. 2005. Functional and structural characterization of recombinant dermcidin-1L, a human antimicrobial peptide. Biochem. Biophys. Res. Commun. 328: 243-250.
11. Morassutti, C., F. De Amicis, A. Bandiera, and S. Marchetti. 2005. Expression of SMAP-29 cathelicidin-like peptide in bacterial cells by intein-mediated system. Protein Expr. Purif. 39: 160-168.
12. Reddy, K. V., R. D. Yedery, and C. Aranha. 2004. Antimicrobial peptides: Premises and promises. Int. J. Antimicrob. Agents 24: 536-547.
13. Rieg, S., C. Garbe, B. Sauer, H. Kalbacher, and B. Schittek. 2004. Dermcidin is constitutively produced by eccrine sweat glands and is not induced in epidermal cells under inflammatory skin conditions. Br. J. Dermatol. 151: 534-539.
14. Sambrook, J. and D. W. Russell. 2000. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, New York.
15. Schittek, B., R. Hipfel, B. Sauer, J. Bauer, H. Kalbacher, S. Stevanovic, et al. 2001. Dermcidin: A novel human antibiotic peptide secreted by sweat glands. Nat. Immunol. 2: 1133-1137.
16. Schröder, J. M. and J. Harder. 2006. Antimicrobial skin peptides and proteins. Cell Mol. Life Sci. 63: 469-486.
17. Senyürek, I., M. Paulmann, T. Sinnberg, H. Kalbacher, M. Deeg, T. Gutsmann, et al. 2009. Dermcidin-derived peptides show a different mode of action than the cathelicidin LL-37 against Staphylococcus aureus. Antimicrob. Agents Chemother. 53: 2499-2509.
18. Sitaram, N. and R. Nagaraj. 1999. Interaction of antimicrobial peptides with biological and model membranes: Structural and charge requirements for activity. Biochim. Biophys. Acta 1462: 29-54.
19. Steffen, H., S. Rieg, I. Wiedemann, H. Kalbacher, M. Deeg, H. G. Sahl, et al. 2006. Naturally processed dermcidin-derived peptides do not permeabilize bacterial membranes and kill microorganisms irrespective of their charge. Antimicrob. Agents Chemother. 50: 2608-2620.
20. Xu, M.-Q. and T. C. Evans Jr. 2005. Recent advances in protein splicing: Manipulating proteins in vitro and in vivo. Curr. Opin. Biotech. 16: 440-446.