High-level expression of an antimicrobial peptide histonin as a natural form by multimerization and furin-mediated cleavage

Applied Microbiology and Biotechnology

Volumn 78, Issue 1, 2008, Pages 123-130

  Kim, Jung Min ^a   Jang, Su A ^a   Yu, Byung Jo ^a   Sung, Bong Hyun ^a   Cho, Ju Hyun ^b   Kim, Sun Chang ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Gyeongsang National University   (South Korea)

Author keywords

Antimicrobial peptide; Furin; Fusion; Histonin; Multimerization

Indexed keywords

ANTIMICROBIAL PEPTIDE; MULTIMERIZATION; PROTEOLYTIC DEGRADATION;

AMINO ACIDS; CELL CULTURE; ESCHERICHIA COLI; TOXICITY;

PEPTIDES;

FURIN; HISTONIN; HYBRID PROTEIN; POLYPEPTIDE ANTIBIOTIC AGENT; UNCLASSIFIED DRUG;

COLIFORM BACTERIUM; GENE EXPRESSION; PEPTIDE; PROTEIN; TOXICITY;

ANTIMICROBIAL ACTIVITY; ARTICLE; BACTERIUM CULTURE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; ESCHERICHIA COLI; HOST RESISTANCE; HUMAN; HUMAN CELL; NONHUMAN; PROTEIN EXPRESSION;

ANTI-BACTERIAL AGENTS; ANTIFUNGAL AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FUNGI; FURIN; GENE EXPRESSION; GRAM-NEGATIVE BACTERIA; GRAM-POSITIVE BACTERIA; MICROBIAL SENSITIVITY TESTS; RECOMBINANT FUSION PROTEINS;

ESCHERICHIA COLI;

EID: 38349084056     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-007-1273-5     Document Type: Article

References (33)

1. Cipakova I, Hostinova E, Gasperik J, Velebny V (2004) High-level expression and purification of a recombinant hBD-1 fused to LMM protein in Escherichia coli. Protein Expr Purif 37:207-212
2. Creemers JW, Usac EF, Bright NA, Van de Loo JW, Jansen E, Van de Ven WJ, Hutton JC (1996) Identification of a transferable sorting domain for the regulated pathway in the prohormone convertase PC2. J Biol Chem 271:25284-25291
3. Dhople V, Krukemeyer A, Ramamoorthy A (2006) The human beta-defensin-3, an antibacterial peptide with multiple biological functions. Biochim Biophys Acta 1758:1499-1512
4. Dürr UH, Sudheendra US, Ramamoorthy A (2006) LL-37, the only human member of the cathelicidin family of antimicrobial peptides. Biochim Biophys Acta 1758:1408-1425
5. Hancock RE, Sahl HG (2006) Antimicrobial and host-defense peptides as new anti-infective therapeutic strategies. Nat Biotechnol 24:1551-1557
6. Ingham AB, Moore RJ (2007) Recombinant production of antimicrobial peptides in heterologous microbial systems. Biotechnol Appl Biochem 47:1-9
7. Iost I, Dreyfus M (1995) The stability of Escherichia coli lacZ mRNA depends upon the simultaneity of its synthesis and translation. EMBO J 14:3252-3261
8. Jenssen H, Hamill P, Hancock RE (2006) Peptide antimicrobial agents. Clin Microbiol Rev 19:491-511
9. Kim HK, Chun DS, Kim JS, Yun CH, Lee JH, Hong SK, Kang DK (2006) Expression of the cationic antimicrobial peptide lactoferricin fused with the anionic peptide in Escherichia coli. Appl Microbiol Biotechnol 72:330-338
10. Lee JH, Minn I, Park CB, Kim SC (1998) Acidic peptide-mediated expression of the antimicrobial peptide buforin II as tandem repeats in Escherichia coli. Protein Expr Purif 12:53-60
11. Lee JH, Kim JH, Hwang SW, Lee WJ, Yoon HK, Lee HS, Hong SS (2000) High-level expression of antimicrobial peptide mediated by a fusion partner reinforcing formation of inclusion bodies. Biochem Biophys Res Commun 277:575-580
12. Lee JH, Kim MS, Cho JH, Kim SC (2002) Enhanced expression of tandem multimers of the antimicrobial peptide buforin II in Escherichia coli by the DEAD-box protein and trxB mutant. Appl Microbiol Biotechnol 58:790-796
13. Li L, Wang JX, Zhao XF, Kang CJ, Liu N, Xiang JH, Li FH, Sueda S, Kondo H (2005) High level expression, purification, and characterization of the shrimp antimicrobial peptide, Ch-penaeidin, in Pichia pastoris. Protein Expr Purif 39:144-151
14. Makovitzki A, Avrahami D, Shai Y (2006) Ultrashort antibacterial and antifungal lipopeptides. Proc Natl Acad Sci U S A 103:15997-16002
15. Mecke A, Lee DK, Ramamoorthy A, Orr BG, Banaszak Holl MM (2005) Membrane thinning due to antimicrobial peptide binding: an atomic force microscopy study of MSI-78 in lipid bilayers. Biophys J 89:4043-4050
16. Merrifield RB (1963) Solid phase peptide synthesis, I, the synthesis of a tetrapeptide. J Am Chem Soc 85:2149-2154
17. Molloy SS, Bresnahan PA, Leppla SH, Klimpel KR, Thomas G (1992) Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen. J Biol Chem 267:16396-16402
18. Mookherjee N, Hancock RE (2007) Cationic host defence peptides: innate immune regulatory peptides as a novel approach for treating infections. Cell Mol Life Sci 64:922-933
19. Moon JY, Henzler-Wildman KA, Ramamoorthy A (2006) Expression and purification of a recombinant LL-37 from Escherichia coli. Biochim Biophys Acta 1758:1351-1358
20. Morassutti C, Amicis FD, Bandiera A, Marchetti S (2005) Expression of SMAP-29 cathelicidin-like peptide in bacterial cells by intein-mediated system. Protein Expr Purif 39:160-168
21. Morin KM, Arcidiacono S, Beckwitt R, Mello CM (2006) Recombinant expression of indolicidin concatamers in Escherichia coli. Appl Microbiol Biotechnol 70:698-704
22. Park CB, Yi KS, Matsuzaki K, Kim MS, Kim SC (2000) Structure-activity analysis of buforin II, a histone H2A-derived antimicrobial peptide: the proline hinge is responsible for the cell-penetrating ability of buforin II. Proc Natl Acad Sci U S A 97:8245-8250
23. Park IY, Cho JH, Kim KS, Kim YB, Kim MS, Kim SC (2004) Helix stability confers salt resistance upon helical antimicrobial peptides. J Biol Chem 279:13896-13901
24. Porcelli F, Buck-Koehntop BA, Thennarasu S, Ramamoorthy A, Veglia G (2006) Structures of the dimeric and monomeric variants of magainin antimicrobial peptides (MSI-78 and MSI-594) in micelles and bilayers, determined by NMR spectroscopy. Biochemistry 45:5793-5799
25. Ramamoorthy A, Thennarasu S, Lee DK, Tan A, Maloy L (2006) Solid-state NMR investigation of the membrane-disrupting mechanism of antimicrobial peptides MSI-78 and MSI-594 derived from magainin 2 and melittin. Biophys J 91:206-216
26. Rao XC, Li S, Hu JC, Jin XL, Hu XM, Huang JJ, Chen JG, Zhu JM, Hu FQ (2004) A novel carrier molecule for high-level expression of peptide antibiotics in Escherichia coli. Protein Expr Purif 36:11-18
27. Reddy KV, Yedery RD, Aranda C (2004) Antimicrobial peptides: premises and promises. Int J Antimicrob Agents 24:536-547
28. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
29. Szynol A, de Haard JJ, Veerman EC, de Soet JJ, van Nieuw Amerongen AV (2006) Design of a peptibody consisting of the antimicrobial peptide dhvar5 and a llama variable heavy-chain antibody fragment. Chem Biol Drug Des 67:425-431
30. Tanaka T, Yada RY (1996) Expression of soluble cloned porcine pepsinogen A in Escherichia coli. Biochem J 315:443-446
31. Wei Q, Kim YS, Seo JH, Jang WS, Lee IH, Cha HJ (2005) Facilitation of expression and purification of an antimicrobial peptide by fusion with baculoviral polyhedrin in Escherichia coli. Appl Environ Microbiol 71:5038-5043
32. Xu X, Jin F, Yu X, Ji S, Wang J, Cheng H, Wang C, Zhang W (2007) Expression and purification of a recombinant antibacterial peptide, cecropin, from Escherichia coli. Protein Expr Purif 53:293-301
33. Zasloff M (2002) Antimicrobial peptides of multicellular organisms. Nature 415:389-395