NMR structure and ion channel activity of the p7 protein from hepatitis C virus

Journal of Biological Chemistry

Volumn 285, Issue 41, 2010, Pages 31446-31461

  Montserret, Roland ^a   Saint, Nathalie ^b   Vanbelle, Christophe ^a,g   Salvay, Andrés Gerardo ^c,h   Simorre, Jean Pierre ^c   Ebel, Christine ^c   Sapay, Nicolas ^a,i   Renisio, Jean Guillaume ^a   Böckmann, Anja ^a   Steinmann, Eike ^d   Pietschmann, Thomas ^d   Dubuisson, Jean ^e   Chipot, Christophe ^f   Penin, François ^a  

^a INSTITUT DE BIOLOGIE ET CHIMIE DES PROTÉINES   (France)

^b CENTRE DE BIOCHIMIE STRUCTURALE   (France)

^c CEA GRENOBLE   (France)

^d INSTITUTE OF INFECTION IMMUNOLOGY   (Germany)

^e UNIV LILLE   (France)

^f CNRS   (France)

^g INSERM   (France)

^h UNIVERSIDAD NACIONAL DE LA PLATA   (Argentina)

ⁱ CENTRE DE RECHERCHES SUR LES MACROMOLÉCULES VÉGÉTALES CERMAV CNRS   (France)

Author keywords

[No Author keywords available]

Indexed keywords

BIOLOGICAL MEMBRANES; DICHROISM; LIPID BILAYERS; MOLECULAR DYNAMICS; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; OLIGOMERIZATION; OLIGOMERS; PHOSPHOLIPIDS; SOAPS (DETERGENTS); THREE DIMENSIONAL; VIRUSES;

AMANTADINE; AMILORIDE; ANTI-VIRAL DRUGS; CIRCULAR DICHROISM; CYTOSOLIC; HEPATITIS C VIRUS; ION CHANNEL; ION-CHANNEL GATING; LYSOLIPIDS; MEMBRANE PROTEINS; MODEL MEMBRANES; MOLECULAR DYNAMICS SIMULATIONS; MONOMERIC FORMS; N-TERMINALS; NMR STRUCTURES; PATCH CLAMP TECHNIQUE; PHOSPHOLIPID BILAYER; SECONDARY STRUCTURE ELEMENTS; SINGLE-CHANNEL; STRUCTURAL DATA; STRUCTURAL FRAMEWORKS; STRUCTURE ANALYSIS; THREE-DIMENSIONAL MODEL; TRANSMEMBRANE HELICES; VIRUS INFECTIVITY;

IONS;

AMANTADINE; AMILORIDE; ION CHANNEL; MEMBRANE PROTEIN; PROTEIN P7; UNCLASSIFIED DRUG; 5-(N,N-HEXAMETHYLENE)AMILORIDE; LIPID BILAYER; P7 PROTEIN, HEPATITIS C VIRUS; VIRUS PROTEIN;

ALPHA HELIX; AMINO TERMINAL SEQUENCE; ARTICLE; CARBON NUCLEAR MAGNETIC RESONANCE; CIRCULAR DICHROISM; HEPATITIS C VIRUS; LIPID BILAYER; MEMBRANE TRANSPORT; MOLECULAR DYNAMICS; MOLECULAR MODEL; NONHUMAN; NUCLEOTIDE SEQUENCE; PATCH CLAMP; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; PROTEIN STRUCTURE; PROTON NUCLEAR MAGNETIC RESONANCE; STRUCTURE ANALYSIS; ANALOGS AND DERIVATIVES; ANTAGONISTS AND INHIBITORS; CHEMICAL STRUCTURE; CHEMISTRY; HEPACIVIRUS; METABOLISM; NUCLEAR MAGNETIC RESONANCE; PHYSIOLOGY; PROTEIN MULTIMERIZATION; PROTEIN QUATERNARY STRUCTURE; SYNTHESIS;

HEPATITIS C VIRUS;

AMILORIDE; AMINO ACID MOTIFS; CIRCULAR DICHROISM; HEPACIVIRUS; ION CHANNELS; LIPID BILAYERS; MODELS, MOLECULAR; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PROTEIN MULTIMERIZATION; PROTEIN STRUCTURE, QUATERNARY; VIRAL PROTEINS;

EID: 77957789317     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M110.122895     Document Type: Article

References (77)

1. Poynard, T., Yuen, M. F., Ratziu, V., and Lai, C. L. (2003) Lancet 362, 2095-2100
2. Simmonds, P., Bukh, J., Combet, C., Deléage, G., Enomoto, N., Feinstone, S., Halfon, P., Inchauspé, G., Kuiken, C., Maertens, G., Mizokami, M., Murphy, D. G., Okamoto, H., Pawlotsky, J. M., Penin, F., Sablon, E., Shin-I, T., Stuyver, L. J., Thiel, H. J., Viazov, S., Weiner, A. J., and Widell, A. (2005) Hepatology 42, 962-973
3. Combet, C., Garnier, N., Charavay, C., Grando, D., Crisan, D., Lopez, J., Dehne-Garcia, A., Geourjon, C., Bettler, E., Hulo, C., Le Mercier, P., Bartenschlager, R., Diepolder, H., Moradpour, D., Pawlotsky, J. M., Rice, C. M., Trépo, C., Penin, F., and Deléage, G. (2007) Nucleic Acids Res. 35, D363-D366
4. Lindenbach, B. D., Thiel, H. J., and Rice, C. M. (2007) in Fields Virology, 5th Ed., Vol. 1 (Knipe, D. M., and Howley, P. M., eds) pp. 1101-1152, Lippincott- Raven Publishers, Philadelphia, PA
5. Moradpour, D., Penin, F., and Rice, C. M. (2007) Nat. Rev. Microbiol. 5, 453-463
6. Carrère-Kremer, S., Montpellier, C., Lorenzo, L., Brulin, B., Cocquerel, L., Belouzard, S., Penin, F., and Dubuisson, J. (2004) J. Biol. Chem. 279, 41384-41392
7. Steinmann, E., Penin, F., Kallis, S., Patel, A. H., Bartenschlager, R., and Pietschmann, T. (2007) PLoS Pathog. 3, e103
8. Carrère-Kremer, S., Montpellier-Pala, C., Cocquerel, L., Wychowski, C., Penin, F., and Dubuisson, J. (2002) J. Virol. 76, 3720-3730
9. Isherwood, B. J., and Patel, A. H. (2005) J. Gen. Virol. 86, 667-676
10. Jones, C. T., Murray, C. L., Eastman, D. K., Tassello, J., and Rice, C. M. (2007) J. Virol. 81, 8374-8383
11. Sakai, A., Claire, M. S., Faulk, K., Govindarajan, S., Emerson, S. U., Purcell, R. H., and Bukh, J. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 11646-11651
12. Zhong, J., Gastaminza, P., Cheng, G., Kapadia, S., Kato, T., Burton, D. R., Wieland, S. F., Uprichard, S. L., Wakita, T., and Chisari, F. V. (2005) Proc. Natl. Acad. Sci. U.S.A. 102, 9294-9299
13. Murray, C. L., Jones, C. T., Tassello, J., and Rice, C. M. (2007) J. Virol. 81, 10220-10231
14. Brohm, C., Steinmann, E., Friesland, M., Lorenz, I. C., Patel, A., Penin, F., Bartenschlager, R., and Pietschmann, T. (2009) J. Virol. 83, 11682-11693
15. Griffin, S. D., Harvey, R., Clarke, D. S., Barclay, W. S., Harris, M., and Rowlands, D. J. (2004) J. Gen. Virol. 85, 451-461
16. Cook, G. A., and Opella, S. J. (2010) Eur. Biophys. J. 39, 1097-1104
17. Gonzalez, M. E., and Carrasco, L. (2003) FEBS Lett. 552, 28-34
18. Pavlović, D., Neville, D. C., Argaud, O., Blumberg, B., Dwek, R. A., Fischer, W. B., and Zitzmann, N. (2003) Proc. Natl. Acad. Sci. U.S.A. 100, 6104-6108
19. Premkumar, A., Wilson, L., Ewart, G. D., and Gage, P. W. (2004) FEBS Lett. 557, 99-103
20. Griffin, S. D., Beales, L. P., Clarke, D. S., Worsfold, O., Evans, S. D., Jaeger, J., Harris, M. P., and Rowlands, D. J. (2003) FEBS Lett. 535, 34-38
21. Steinmann, E., Whitfield, T., Kallis, S., Dwek, R. A., Zitzmann, N., Pietschmann, T., and Bartenschlager, R. (2007) Hepatology 46, 330-338
22. Griffin, S., Stgelais, C., Owsianka, A. M., Patel, A. H., Rowlands, D., and Harris, M. (2008) Hepatology 48, 1779-1790
23. Luik, P., Chew, C., Aittoniemi, J., Chang, J., Wentworth, P., Jr., Dwek, R. A., Biggin, P. C., Vénien-Bryan, C., and Zitzmann, N. (2009) Proc. Natl. Acad. Sci. U.S.A. 106, 12712-12716
24. Clarke, D., Griffin, S., Beales, L., Gelais, C. S., Burgess, S., Harris, M., and Rowlands, D. (2006) J. Biol. Chem. 281, 37057-37068
25. Patargias, G., Zitzmann, N., Dwek, R., and Fischer, W. B. (2006) J. Med. Chem. 49, 648-655
26. StGelais, C., Foster, T. L., Verow, M., Atkins, E., Fishwick, C. W., Rowlands, D., Harris, M., and Griffin, S. (2009) J. Virol. 83, 7970-7981
27. Haqshenas, G., Dong, X., Ewart, G., Bowden, S., and Gowans, E. J. (2007) Virology 360, 17-26
28. Chew, C. F., Vijayan, R., Chang, J., Zitzmann, N., and Biggin, P. C. (2009) Biophys. J. 96, L10-L12
29. Combet, C., Blanchet, C., Geourjon, C., and Deléage, G. (2000) Trends Biochem. Sci. 25, 147-150
30. Thompson, J. D., Higgins, D. G., and Gibson, T. J. (1994) Nucleic Acids Res. 22, 4673-4680
31. Saint, N., Montserret, R., Chipot, C., and Penin, F. (2009) Methods Mol. Biol. 510, 125-143
32. Saint, N., Lou, K. L., Widmer, C., Luckey, M., Schirmer, T., and Rosenbusch, J. P. (1996) J. Biol. Chem. 271, 20676-20680
33. Hodgkin, A. L., and Huxley, A. F. (1947) J. Physiol. 106, 341-367
34. Le Dain, A. C., Saint, N., Kloda, A., Ghazi, A., and Martinac, B. (1998) J. Biol. Chem. 273, 12116-12119
35. Hamill, O. P., Marty, A., Neher, E., Sakmann, B., and Sigworth, F. J. (1981) Pflugers Arch. 391, 85-100
36. Salvay, A. G., and Ebel, C. (2006) in Analytical Ultracentrifugation VIII (Wondrey, C., and Cölfen, H., eds) Progr. Colloid Polymer Sci. Vol. 131, pp. 399-419, Springer, New York
37. Whitmore, L., and Wallace, B. A. (2004) Nucleic Acids Res. 32, W668-W673
38. Penin, F., Geourjon, C., Montserret, R., Böckmann, A., Lesage, A., Yang, Y. S., Bonod-Bidaud, C., Cortay, J. C., Nègre, D., Cozzone, A. J., and Delé- age, G. (1997) J. Mol. Biol. 270, 496-510
39. Favier, A., Brutscher, B., Blackledge, M., Galinier, A., Deutscher, J., Penin, F., and Marion, D. (2002) J. Mol. Biol. 317, 131-144
40. Melckebeke, H. V., Vreuls, C., Gans, P., Filée, P., Llabres, G., Joris, B., and Simorre, J. P. (2003) J. Mol. Biol. 333, 711-720
41. Wuthrich, K. (1986) NMR of Proteins and Nucleic Acids, John Wiley & Sons, Inc., New York
42. Medvedeva, S., Simorre, J. P., Brutscher, B., Guerlesquin, F., and Marion, D. (1993) FEBS Lett. 333, 251-256
43. Merutka, G., Dyson, H. J., and Wright, P. E. (1995) J. Biomol. NMR 5, 14-24
44. Thanabal, V., Omecinsky, D. O., Reily, M. D., and Cody, W. L. (1994) J. Biomol. NMR 4, 47-59
45. Schwieters, C. D., Kuszewski, J. J., Tjandra, N., and Clore, G. M. (2003) J. Magn. Reson. 160, 65-73
46. Koradi, R., Billeter, M., and Wüthrich, K. (1996) J. Mol. Graph. 14, 51-55
47. Laskowski, R. A., Rullmannn, J. A., MacArthur, M. W., Kaptein, R., and Thornton, J. M. (1996) J. Biomol. NMR 8, 477-486
48. Vakser, I. A., Matar, O. G., and Lam, C. F. (1999) Proc. Natl. Acad. Sci. U.S.A. 96, 8477-8482
49. Humphrey, W., Dalke, A., and Schulten, K. (1996) J. Mol. Graph. 14, 33-38
50. Phillips, J. C., Braun, R., Wang, W., Gumbart, J., Tajkhorshid, E., Villa, E., Chipot, C., Skeel, R. D., Kalé, L., and Schulten, K. (2005) J Comput. Chem. 26, 1781-1802
51. MacKerell, A. D., Bashford, D., Bellott, M., Jr., Dunbrack, R. L., Evanseck, J. D., Field, M. J., Fischer, S., Gao, J., Guo, H., Ha, S., Joseph-McCarthy, D., Kuchnir, L., Kuczera, K., Lau, F. T. K., Mattos, C., Michnick, S., Ngo, T., Nguyen, D. T., Prodhom, B., Reiher, W. E., Roux, B., Schlenkrich, M., Smith, J. C., Stote, R., Straub, J., Watanabe, M., Wiorkiewicz-Kuczera, J., Yin, D., and Karplus, M. (1998) J. Phys. Chem. B 102, 3586-3616
52. Feller, S. E., Zhang, Y. H., Pastor, R. W., and Brooks, B. R. (1995) J. Chem. Phys. 103, 4613-4621
53. Darden, T. A., York, D. M., and Pedersen, L. G. (1993) J. Chem. Phys. 98, 10089-10092
54. Tuckerman, M. E., Berne, B. J., and Martyna, G. J. (1992) J. Phys. Chem. 97, 1990-2001
55. Møller, J. V., and le Maire, M. (1993) J. Biol. Chem. 268, 18659-18672
56. Buck, M. (1998) Q. Rev. Biophys. 31, 297-355
57. Montserret, R., Aubert-Foucher, E., McLeish, M. J., Hill, J. M., Ficheux, D., Jaquinod, M., van der Rest, M., Deléage, G., and Penin, F. (1999) Biochemistry 38, 6479-6488
58. Wishart, D. S., Sykes, B. D., and Richards, F. M. (1992) Biochemistry 31, 1647-1651
59. Frishman, D., and Argos, P. (1995) Proteins 23, 566-579
60. Chipot, C., Jaffe, R., Maigret, B., Pearlman, D. A., and Kollman, P. A. (1996) J. Am. Chem. Soc. 118, 11217-11224
61. Castelain, S., Bonte, D., Penin, F., François, C., Capron, D., Dedeurwaerder, S., Zawadzki, P., Morel, V., Wychowski, C., and Duverlie, G. (2007) J. Med. Virol. 79, 144-154
62. Cordes, F. S., Tustian, A. D., Sansom, M. S., Watts, A., and Fischer, W. B. (2002) Biochemistry 41, 7359-7365
63. Helluin, O., Dugast, J. Y., Molle, G., Mackie, A. R., Ladha, S., and Duclohier, H. (1997) Biochim. Biophys. Acta 1330, 284-292
64. Saint, N., Marri, L., Marchini, D., and Molle, G. (2003) Peptides 24, 1779-1784
65. Grice, A. L., Kerr, I. D., and Sansom, M. S. (1997) FEBS Lett. 405, 299-304
66. Fischer, W. B., Forrest, L. R., Smith, G. R., and Sansom, M. S. (2000) Biopolymers 53, 529-538
67. Chew, C. F., Guy, A., and Biggin, P. C. (2008) Biophys. J. 95, 5627-5636
68. Park, S. H., Mrse, A. A., Nevzorov, A. A., Mesleh, M. F., Oblatt-Montal, M., Montal, M., and Opella, S. J. (2003) J. Mol. Biol. 333, 409-424
69. Park, S. H., De Angelis, A. A., Nevzorov, A. A., Wu, C. H., and Opella, S. J. (2006) Biophys. J. 91, 3032-3042
70. Montal, M. (2003) FEBS Lett. 552, 47-53
71. StGelais, C., Tuthill, T. J., Clarke, D. S., Rowlands, D. J., Harris, M., and Griffin, S. (2007) Antiviral Res. 76, 48-58
72. Meshkat, Z., Audsley, M., Beyer, C., Gowans, E. J., and Haqshenas, G. (2009) J. Viral Hepat. 16, 187-194
73. Tang, Y., Zaitseva, F., Lamb, R. A., and Pinto, L. H. (2002) J. Biol. Chem. 277, 39880-39886
74. Unwin, N. (1995) Nature 373, 37-43
75. Perozo, E., Cortes, D. M., and Cuello, L. G. (1999) Science 285, 73-78
76. Biggin, P. C., and Sansom, M. S. (2001) Curr. Biol. 11, R364-R366
77. Kuiken, C., Mizokami, M., Deleage, G., Yusim, K., Penin, F., Shin-I, T., Charavay, C., Tao, N., Crisan, D., Grando, D., Dalwani, A., Geourjon, C., Agrawal, A., and Combet, C. (2006) Hepatology 43, 1157-1165