Enhanced periplasmic expression of high affinity humanized scFv against Hepatitis B surface antigen by codon optimization

Protein Expression and Purification

Volumn 74, Issue 2, 2010, Pages 272-279

  Tiwari, Ashutosh ^a   Sankhyan, Anurag ^a   Khanna, Navin ^b   Sinha, Subrata ^a  

^a ALL INDIA INSTITUTE OF MEDICAL SCIENCES   (India)

^b INTERNATIONAL CENTRE FOR GENETIC ENGINEERING AND BIOTECHNOLOGY ICGEB   (India)

Author keywords

Antibody; Codon optimization; Hepatitis B; Humanization; Periplasmic expression; scFv

Indexed keywords

HEPATITIS B SURFACE ANTIGEN; IMMUNOGLOBULIN FRAGMENT; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

AMINO ACID SEQUENCE; ANIMAL; ARTICLE; CODON; CYTOPLASM; ESCHERICHIA COLI; GENETICS; IMMUNOLOGY; METABOLISM; MOLECULAR GENETICS; MOUSE; NUCLEOTIDE SEQUENCE; PROTEIN ENGINEERING;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CODON; ESCHERICHIA COLI; HEPATITIS B SURFACE ANTIGENS; IMMUNOGLOBULIN FRAGMENTS; MICE; MOLECULAR SEQUENCE DATA; PERIPLASM; PROTEIN ENGINEERING; SINGLE-CHAIN ANTIBODIES;

ESCHERICHIA COLI; PROKARYOTA;

EID: 77957749181     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2010.06.006     Document Type: Article

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