High-level periplasmic expression in Escherichia coli using a eukaryotic signal peptide: Importance of codon usage at the 5’ end of the coding sequence

Protein Expression and Purification

Volumn 20, Issue 2, 2000, Pages 252-264

  Humphreys, David P ^a   Sehdev, Mukesh ^a   Chapman, Andrew P ^a   Ganesh, Ravindra ^a   Smith, Bryan J ^a   King, Lloyd M ^a   Glover, David J ^a   Reeks, Dominic G ^a   Stephens, Paul E ^a  

^a UCB PHARMA   (Belgium)

Author keywords

Codon usage; E. coli; Eukaryotic signal peptide; Fab ; MRNA structure

Indexed keywords

BACTERIAL GENE; CODON; CYTOPLASM; EUKARYOTIC CELL; GENE EXPRESSION; MUTAGENESIS; PROKARYOSIS; SIGNAL PEPTIDE;

ESCHERICHIA COLI;

EID: 0033727599     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1006/prep.2000.1286     Document Type: Article

References (45)

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