SMC, a simple method to rapidly assemble multiple fragments into one construct

Frontiers in Bioscience - Elite

Volumn 2 E, Issue 3, 2010, Pages 1105-1114

  Tan, Guihong ^a   Tan, Change ^a  

^a UNIVERSITY OF MISSOURI   (United States)

Author keywords

Construct; Multiple piece cloning; Split marker; Yeast homologous recombination

Indexed keywords

FUNGAL DNA; PRIMER DNA;

ARTICLE; CHEMISTRY; NUCLEOTIDE SEQUENCE; PLASMID; POLYMERASE CHAIN REACTION; YEAST;

BASE SEQUENCE; DNA PRIMERS; DNA, FUNGAL; PLASMIDS; POLYMERASE CHAIN REACTION; YEASTS;

EID: 77957345421     PISSN: 19450494     EISSN: 19450508     Source Type: Journal    
DOI: 10.2741/e169     Document Type: Article

References (20)

1. Cohen, S. N., A. C. Chang, H. W. Boyer & R. B. Helling: Construction of biologically functional bacterial plasmids in vitro. Proc Natl Acad Sci U S A, 70, 3240-4 (1973)
2. Murphy, K. C.: Use of bacteriophage lambda recombination functions to promote gene replacement in Escherichia coli. J Bacteriol, 180, 2063-71 (1998) (Pubitemid 28212691)
3. Zhang, Y., F. Buchholz, J. P. Muyrers & A. F. Stewart: A new logic for DNA engineering using recombination in Escherichia coli. Nat Genet, 20, 123-8 (1998) (Pubitemid 28455443)
4. Gibson, D. G., G. A. Benders, K. C. Axelrod, J. Zaveri, M. A. Algire, M. Moodie, M. G. Montague, J. C. Venter, H. O. Smith & C. A. Hutchison, 3rd: One-step assembly in yeast of 25 overlapping DNA fragments to form a complete synthetic Mycoplasma genitalium genome. Proc Natl Acad Sci U S A, 105, 20404-9 (2008)
5. Li, M. Z. & S. J. Elledge: Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat Methods, 4, 251-6 (2007) (Pubitemid 46358876)
6. Kotera, I. & T. Nagai: A high-throughput and single-tube recombination of crude PCR products using a DNA polymerase inhibitor and type IIS restriction enzyme. J Biotechnol, 137, 1-7 (2008)
7. Bitinaite, J., M. Rubino, K. H. Varma, I. Schildkraut, R. Vaisvila & R. Vaiskunaite: USER friendly DNA engineering and cloning method by uracil excision. Nucleic Acids Res, 35, 1992-2002 (2007) (Pubitemid 47061665)
8. Gibson, D. G., G. A. Benders, C. Andrews-Pfannkoch, E. A. Denisova, H. Baden-Tillson, J. Zaveri, T. B. Stockwell, A. Brownley, D. W. Thomas, M. A. Algire, C. Merryman, L. Young, V. N. Noskov, J. I. Glass, J. C. Venter, C. A. Hutchison, 3rd & H. O. Smith: Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Science, 319, 1215-20 (2008) (Pubitemid 351323011)
9. Gibson, D. G., L. Young, R. Y. Chuang, J. C. Venter, C. A. Hutchison, 3rd & H. O. Smith: Enzymatic assembly of DNA molecules up to several hundred kilobases. Nat Methods, 6, 343-5 (2009)
10. Ma, H., S. Kunes, P. J. Schatz & D. Botstein: Plasmid construction by homologous recombination in yeast. Gene, 58, 201-16 (1987) (Pubitemid 17164063)
11. Gibson, D. G.: Synthesis of DNA fragments in yeast by one-step assembly of overlapping oligonucleotides. Nucleic Acids Res, 37, 6984-90 (2009)
12. Hua, S. B., M. Qiu, E. Chan, L. Zhu & Y. Luo: Minimum length of sequence homology required for in vivo cloning by homologous recombination in yeast. Plasmid, 38, 91-6 (1997) (Pubitemid 27435623)
13. Shao, Z. & H. Zhao: DNA assembler, an in vivo genetic method for rapid construction of biochemical pathways. Nucleic Acids Res, 37, e16 (2009)
14. Tan, G., M. Chen, C. Foote & C. Tan: Temperaturesensitive mutations made easy: generating conditional mutations by using temperature-sensitive inteins that function within different temperature ranges. Genetics, 183, 13-22 (2009)
15. Gietz, R. D. & R. A. Woods: Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol, 350, 87-96 (2002) (Pubitemid 34619485)
16. Hoffman, C. S. & F. Winston: A ten-minute DNA preparation from yeast efficiently releases autonomous plasmids for transformation of Escherichia coli. Gene, 57, 267-72 (1987) (Pubitemid 18007116)
17. Zeidler, M. P., C. Tan, Y. Bellaiche, S. Cherry, S. Hader, U. Gayko & N. Perrimon: Temperature-sensitive control of protein activity by conditionally splicing inteins. Nat Biotechnol, 22, 871-6 (2004) (Pubitemid 38886607)
18. Rorth, P.: Gal4 in the Drosophila female germline. Mech Dev, 78, 113-8 (1998) (Pubitemid 28566217)
19. Wach, A., A. Brachat, C. Alberti-Segui, C. Rebischung & P. Philippsen: Heterologous HIS3 marker and GFP reporter modules for PCR-targeting in Saccharomyces cerevisiae. Yeast, 13, 1065-75 (1997) (Pubitemid 27368517)
20. Fairhead, C., B. Llorente, F. Denis, M. Soler & B. Dujon: New vectors for combinatorial deletions in yeast chromosomes and for gap-repair cloning using 'splitmarker' recombination. Yeast, 12, 1439-57 (1996) (Pubitemid 26393225)